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Energy Relationships 1. Photosynthesis: Chloroplast: energy from the sun convert inorganic compounds to organic compounds. 2. Respiration: mitochondria: convert energy from organic compounds to form compounds more easily used by the cell (ATP) Biochemical pathways: a series of reactions in which the product of one reaction is consumed in the next reaction. ex. photosynthesis Autotrophs vs. • Make own food from organic compounds • Examples: green plants and algae Heterotrophs • Cannot make their own organic compounds • Examples: bacteria and humans ***all life depends on the sun*** autotrophs use CO2 (carbon dioxide) and H2O (water) to make glucose giving off O2 heterotrophs use C6H12O6 (glucose) and O2 (oxygen) giving off CO2 and H2O materials are constantly recycling energy not recycled; must be supplied constantly Photosynthesis Worksheet (read and answer) Chloroplasts Photosynthesis is a process in which sunlight energy is used to make glucose. The site of photosynthesis is in the chloroplast – a organelle found in the leaves of green plants. The main functions of chloroplasts are to produce food (glucose) during photosynthesis, and to store food energy. Chloroplasts contain the pigment, chlorophyll. Chlorophyll absorbs most of the colors in the color spectrum, and reflects only green and yellow wavelengths of light. This is why we see leaves as green or yellow – because these colors are reflected into our eyes. 1. What is photosynthesis? 2. Where does photosynthesis occur? 3. What are chloroplasts and where are they found? 4. What are the two main functions of chloroplasts? 5. Why doe most leaves appear green? 6. What is the primary pigment found in the chloroplast? Photosynthesis Glucose is another name for sugar. The molecular formula for glucose in C6H12O6. Plants make sugar by using the energy from sunlight to transform CO2 from the air with water from the ground into glucose. This process, called photosynthesis, occurs in the chloroplast of the plant cell. During this process, oxygen (O2) is created as a waste product and is released into the air for us to breath. The formula for photosynthesis is: CO2 + H2O + sunlight ---- C6H12O6 + O2 This formula says that carbon dioxide and water molecules are combined with the energy from sunlight to produce sugar and oxygen. The reactants in photosynthesis (what is used) are CO2, water and sun. The plant gets water from the ground through its roots. The plant collects carbon dioxide from the air. Much of the carbon dioxide comes from living organisms that exhale it, but some also comes from factory smokestacks and car fumes. 7. What is the formula for photosynthesis? 8. What three things are used to make glucose in photosynthesis? 9. Where does the water come from? 10. Where does the water enter the plant? 11. What are some sources of CO2? 12. What type of energy does the plant use to convert CO2 and H2O into sugar? The products (what is made) are glucose and oxygen. The glucose produced is used by the plant for energy and growth. We also use this glucose by eating plants. The oxygen produced is released into the air for us to breath. Photosynthesis is essential for all life on earth, because it provides food and oxygen. 13. What is produced in photosynthesis? 14. What is the glucose used for? 15. What is the oxygen used for? Photosynthesis I. Capturing the Energy in Light Photosynthesis summary equation: 6CO2 carbon dioxide + 6H2O water + light C6H12O6 glucose + 6O2 oxygen • • • • • • II. Phases (controlled by enzymes) 1. Light Reactions also called photolysis Light absorption in grana of chloroplasts Light and Pigments chlorophyll trap energy from light (sun or artificial) water molecules split (photolysis) and oxygen is released from water; proved with oxygen-18 leftover energy produced stored in ATP grana Light and Pigments light from sun appears clear/ opaque but is composed of many colors least energy red orange yellow visible spectrum green blue blue most energy violet blue/violet is most absorbed, and used most by the plant green/yellow is most reflected, leased used • chlorophyll a: directly involved in light reactions • Accessory Pigments • chlorophyll b: accessory pigment carotenoids: orange The Absorption of Chlorophyll A pigment is a substance that absorbs and reflects light of particular wavelengths. For example, the yellow-green color of a leaf is due to a pigment in the leaf called chlorophyll. When white light (which contains all of the colors of the spectrum) shines on chlorophyll, the chlorophyll absorbs most of the red, orange, blue, and violet and reflects most of the green and yellow. That is why you see a yellow-green color. Think of a pigment as a sponge that soaks up all of the other colors of the spectrum except the one you see. A spectrophotometer is an instrument that is used to measure the amount of light absorbed by a pigment. Below is a graph showing the percentage of light energy reflected for the absorption spectrum for chlorophyll. The highest peaks represent colors that chlorophyll absorbs the most. Therefore, they are the least visible. % of light absorbed 100 ABSORPTION SPECTRUM FOR CHLOROPHYLL Chlorophyll a 50 .- ChtorophyH b I I I I. \ \ . 0 3000 ' 5000 4000 violet blue green 6000 yellow orange 7000 red Wavelength (in angstroms) Use the above ·graph to answer the following questions. I . Which of the colors absorbed by chlorophyll is least visible? ________________________________ 2. What is its approximate wavelength? _________________________________ 3. What percentage of light energy absorbed does this peak represent? _____________________ 4. How much of this color is being reflected? ___________________________ 5. What percentage of light energy absorbed by chlorophyll does the orange spectrum peak represent? _____________ 6. Why would you say there are no peaks in the range between 5000 angstroms and 6100 angstroms? _________________________ 7. Are you able to see the light in the yellow-green part of the spectrum? Explain why. ____________, _______________________________________________________________________________________________ 8. Arrange the colors in the absorption spectrum of chlorophyll in order of their visibility. Place the most visible color first. ________________________________________________________________________________ PERCENT OF LIGHT ENERGY REFLECTED BY CHLOROPHYLL Percent of light energy reflected 100 90 _ _, .) 80 r---- - 7060 50 40- ,.---- r--- 30 20 10- .11 violet blue green yellow orange red wavelength ( in angstroms) Use the bar graph, which shows the percentage of light energy reflected by chlorophyll, to answer the following questions. The graph was derived from the chlorophyll absorption spectrum. 9. Which color in this spectrum is most visible? ------------- -----------------------10. What is the approximate percentage of light energy reflected for this color?------- ------11. What percentage.9f light energy absorbed does this represent? --------------------------- 12. If everything above 50 percent of light energy reflected is visible to the human eye, is red light, part of the mixture of colors seen in light reflected by chlorophyll? ----- - ---- -- .. pigments easily separated by chromatography The diagram below represents the results of a laboratory procedure. This procedure is used to (1) separate molecules in a liquid mixture (2) detect glucose in a solution (3) determine the rate of photosynthesis in plants (4) examine the gene sequences of organisms 2. Dark Reactions stroma * also called carbon fixation •take place in stroma of chloroplasts; carbon dioxide is fixed in carbohydrate •PGAL (phosphoglyceraldehyde; 3 carbon) is formed * glucose is synthesized from two 3-C PGAL •molecules; called C-3 pathway Alternative Pathways **** C-4 pathway; fix CO2 in 4 carbon compounds ex. corn, millet, crabgrass ****CAM; take in carbon dioxide at night and fix it into organic compounds ex. cactus, pineapple in hot dry climates Rate of Photosynthesis Affected by: *. amount of carbon dioxide *. amount of water *. amount of light Cellular Respiration making ATP by breaking down organic compounds summary equation: C6H12O6 + 6O2 glucose oxygen energy 6CO2 + carbon dioxide 6H2O water + Steps: 1.Glycolysis 2. ANAEROBIC Respiration (no oxygen) OR AEROBIC Respiration (oxygen) 36 ATP 1. Glycolysis occurs outside the MITOCHONDRIA(in the cytoplasm) glucose + 2 ATP 2 PGAL 2 pyruvic acid + 4 ATP Net gain: 2 ATP 2. Aerobic Respiration: if OXYGEN is available occurs in the MITOCHONDRIA 2 pyruvic acid + oxygen carbon dioxide + water + 34 ATP Net gain of ATP during aerobic respiration = 34 Total ATP from both stages = 36 Base your answers to questions 1 and 2 on the word equations below and on your knowledge of biology. The equations represent two biochemical processes that occur in living organisms. The letter X represents a molecule produced from process 1. Process 1: oxygen + glucose → carbon dioxide + water + X Process 2: carbon dioxide + water → oxygen + glucose 1. Identify the molecule represented by letter X in process 1. __________________________________ 2. Which process occurs in the cells of a green plant leaf? (1) process 1, only (2) neither process 1 nor process 2 (3) process 2, only (4) both process 1 and process 2 2. ANAEROBIC RESPIRATION Fermentation: no oxygen available a. Lactic Acid Fermentation occurs in MUSCLE cells glucose pyruvic acid lactic acid + 2 ATP muscle sore, cramps b. Alcoholic Fermentation some plant cells, yeast glucose pyruvic acid ethyl alcohol + 2 ATP WINE AND BREAD making The laboratory setups represented below were used to investigate the effect of temperature on cellular respiration in yeast single-celled organism). Each of two flasks containing equal amounts of a yeast-glucose solution was submerged in a water bath, one kept at 20°C and one kept at 35°C. The number of gas bubbles released from the glass tube in each setup was observed and the results were recorded every 5 minutes for a period of 25 minutes. The data are summarized in the table below. State one relationship between temperature and the rate of gas production in yeast. [1] Identity the gas that would be produced by the process taking place in both laboratory setups. [1] _________________________________ Photosynthesis Multiple Choice Questions 1. A green plant is kept in a brightly lighted area for 48 hours. What will most likely occur if the light intensity is then reduced slightly during the next 48 hours? 1. Photosynthesis will stop completely. 2. The rate at which nitrogen is used by the plant will increase. 3. The rate at which oxygen is released from the plant will decrease. 4. Glucose production inside each plant cell will increase. 2. An inorganic molecule required by green plants for the process of photosynthesis is 1. oxygen 2. starch 3. carbon dioxide 4. Glucose . 3. If the leaves of a geranium plant receive an adequate supply of raw materials, which graph below shows how the rate of photosynthesis is related to increasing light intensity received by the plant? 1. 2. 3. 4. Which activity occurs in the process of photosynthesis? 1. Chemical energy from organic molecules is converted into light energy. 2. Organic molecules are obtained from the environment. 3. Organic molecules are converted into inorganic food molecules. 4. Light energy is converted into the chemical energy of organic molecules. 5. Photosynthesis is the process by which 1. the potential energy of simple sugars is transferred to ATP molecules 2. simple sugars are gradually broken down to form lactic acid or alcohol 3. two simple sugar molecules combine to form maltose and water 4. light energy is converted into the chemical energy of simple sugars 4. 4. decomposition 6. 8. Which biological process is the main source of atmospheric oxygen? 1. respiration 2. photosynthesis 3. hydrolysis 4. Synthesis 9. The graph represents the absorption spectrum of chlorophyll. The graph indicates that the energy used in photosynthesis is most likely obtained from which regions of the spectrum? 1. yellow and orange red 2. violet blue and green 3. orange red and violet blue 4. green and yellow 10. Eating a sweet potato provides energy for human metabolic processes. The original source of this energy is the energy 1. in protein molecules stored within the potato 2. from starch molecules absorbed by the potato plant 3. made available by photosynthesis 4. in vitamins and minerals found in the soil 11. The mass of some corn plants at the end of their growth period was 6 tons per acre. Most of this mass was produced from 1. water and organic compounds absorbed from the soil 2. minerals from the soil and oxygen from the air 3. minerals and organic materials absorbed from the soil 4. water from the soil and carbon dioxide from the air 12. The dense needles of Douglas fir trees can prevent most light from reaching the forest floor. This situation would have the most immediate effe 1. producers 2. carnivores 3. herbivores 4. decomposers 13. The diagram represents part of a life process in a leaf chloroplast. If the process illustrated in the diagram is interrupted by a chemical at point X, there would be an immediate effect on the release of 1.chlorophyll 2.nitrogen 3.carbon dioxide 4.oxygen 14. The equation below represents a summary of a biological process. carbon dioxide + water → glucose + water + oxygen This process is completed in 1. mitochondria 2. ribosomes 3. cell membranes 4. Chloroplasts 15. Which process is directly used by autotrophs to store energy in glucose? 1. diffusion 2. photosynthesis 3. respiration 4. active transport 16. Organisms that have the ability to use an atmospheric gas to produce an organic nutrient are known as 1. herbivores 2. decomposers 3. carnivores 4. autotrophs 17. Leaves of green plants contain openings known as stomates, which are opened and closed by specialized cells allowing for gas exchange between the leaf and the outside environment. Which phrase best represents the net flow of gases involved in photosynthesis into and out of the leaf through these openings on a sunny day? 1. carbon dioxide moves in; oxygen moves out 2. carbon dioxide and oxygen move in; ozone moves out 3. oxygen moves in; nitrogen moves out 4. water and ozone move in; carbon dioxide moves out 18. Which process usually uses carbon dioxide molecules? 1. cellular respiration 2. asexual reproduction 3. active transport 4. autotrophic nutrition 19. The graph below shows the results of an experiment in which a container of oxygen-using bacteria and strands of a green alga were exposed to light of different colors. Which statement best explains the results of this experiment? 1. 2. 3. 4. The rate of photosynthesis is affected by variations in the light. In all environments light is a vital resource. The activities of bacteria and algae are not related. Uneven numbers and types of species can upset ecosystem stability. 20. Which process provides the initial energy to support all the levels in the energy pyramid shown below? 1.circulation 2.photosynthesis 3.active transport 4.Digestion Constructed Response Questions The Control of Transpiration Plants normally lose water from openings (stomates) in their leaves. The water loss typically occurs during daylight hours when plants are exposed to the Sun. This water loss, known as transpiration, is both beneficial and harmful to plants. Scientists believe wind and high temperatures increase the rate of transpiration, but the size of each stomate opening can be regulated. Reducing the size of the openings during drought conditions may help reduce the dehydration and wilting that would otherwise occur. A leaf may lose more than its own weight in water each day. Transpiration also lowers the internal temperature of the leaf as water evaporates. On hot days, temperatures in the leaves may be from 3° to 15°C cooler than the outside air. With stomates open, vital gases may be exchanged between the leaf tissues and the outside environment. Researchers have also found many plants that use another response when leaf temperatures rise. Special molecules known as heat shock proteins are produced by plant cells and help to hold enzymes in their functional shapes. Identify the specific leaf structures that regulate the opening and closing of stomates. Identify two of the "vital gases" that are exchanged between leaf tissues and the outside environment. State one way transpiration is beneficial to plants. In some land plants, guard cells are found only on the lower surfaces of the leaves. In some water plants, guard cells are found only on the upper surfaces of the leaves. Explain how guard cells in both land and water plants help maintain homeostasis. In your answer be sure to: • identify one function regulated by the guard cells in leaves • explain how guard cells carry out this function • give one possible evolutionary advantage of the position of the guard cells on the leaves of land plants _______________________________________________________________________________________________________________ _______________________________________________________________________________________________________________ _______________________________________________________________________________________________________________ _______________________________________________________________________________________________________________ Base your answer to the following question on the investigation described below and on your knowledge of biology. As part of an investigation, 10 bean seedlings in one setup were grown in the dark, while 10 seedlings in another setup were grown in sunlight. All other growth conditions were kept the same in both setups. The seedlings grown in the dark were white with long, slender stems. These seedlings soon died. The seedlings grown in the sunlight were green and healthy. Identify the independent variable in this investigation ______________________________________________________ The diagram below shows the setup of an experiment. A)Using one or more complete sentences, state a problem that could be investigated using this experimental setup. _________________________________________________________________________________________________________________________