Download Specification sheet

Rev: A
Release Date: 05/25/2015
IVD
Cyclin E1(EP126)
Intended Use:
Clone
EP126
Source
Rabbit Monoclonal
Cat #
PR178-6ml RTU
PR178-3ml RTU
CR178-0.1ml Conc
CR178-0.5ml Conc
HAR178-6ml RTU
HAR178-3ml RTU
This antibody is intended for use to qualitatively
Regulatory
IVD
identify Cyclin E1antigen by light microscopy in
Status
formalin fixed, paraffin embedded tissue sections
using immunohistochemical detection methodology. Interpretation of any positive or negative staining must be
complemented with the evaluation of proper controls and must be made within the context of the patient’s
clinical history and other diagnostic tests. A qualified pathologist must perform evaluation of the test.
Summary and Explanation:
Cyclin E1 is a member of the cyclin E family that can associate with and activate cyclin-dependent kinase
Cdk2. Expression of cyclin E1 is essential for the control of the cell cycle at the late G1 and early S phase.
Ubiquination by the Cul-3 pathway and Fbw7 regulatescyclin E1 levels and is critically important in normal
cells.
In normal cells, cyclin E1 protein expression is tightly controlled through a combination of transcriptional and
proteolytic regulatory processes. However, in many types of human tumors, cyclin E1 expression is frequently
dysregulated, including overexpression, non-periodic expression relative to cell division, and generation of low
molecular weight (LMW) derivatives.
Several studies have consistently demonstrated that Cyclin E1 is associated with disease progression or
patient survival in various malignancies including carcinomas of the breast, bladder, colon, and ovary. A recent
study indicated that cyclin E amplification/overexpression is responsible for trastuzumab resistance in HER2
positive breast cancer patients.
Immunogen:A synthetic peptide corresponding to residues of human Cyclin E1 protein.
Isotype:
Rabbit IgG
Reagent Provided:
Concentrated format: Antibody to Cyclin E1 is diluted in antibody diluent, with 1% bovine serum
albumin (BSA) and 0.05% sodium azide (NaN3). Recommended dilutions: 1:50 –
1:100.The antibody dilution and protocol may vary depending on the specimen
preparation and specific application. Optimal conditions should be
US Office: 538, Selby Lane, Livermore, CA- 94551 USA, Ph: +1 925-218-6939
Corporate Office:CDC Towers, 3rd Floor, B-Block, Plot 10/8, Nacharam IDA, Road #5, Nacharam, Hyderabad-76, India.
Phone: 040-27015544/33,Fax:040-2701 5544 ,
E-Mail:[email protected]: www.pathnsitu.com
determined by individual laboratory.
Pre-diluted format: PathnSitu ready to use antibodies are pre tittered to optimal staining
conditions. Further dilution may loose the activity and may yield to sub
optimal staining.
Storage Recommendations: Store at 2°-8°C. Do not use after expiration date provided on the vial.
Staining Recommendations:
Antigen Retrieval Solution: Use CitrateBuffer(PathnSitu Cat # PS009) as antigen retrieval solution
Heat Retrieval Method: Retrieve sections under steam pressure for 15
min using PathnSitu’s MERS (Multi Epitope Retrieval System) then allow
solution to cool for 10 minutes then transfer tissue sections/slides to
distilled water.
Primary Antibody:
Cover the tissue sections with primary antibody and incubate for 30
min at room temperature when used PathnSitu PolyExcel Detection
System.
Detection System:
Refer to PathnSitu PolyExcel detection system protocol or manufacturer’s
detection kit staining protocol when used other vendor detection system.
Cellular Localization:
Nuclear, Cytoplasmic reactivity also observed.
Positive Control:
Placenta, Breast Cancer
Troubleshooting:
Follow the antibody specific protocol recommendations according to data sheet
provided. If unusual results occur, contact PathnSitu Technical Support at 0402701 5544 or [email protected].
Limitations and Warranty: There are no warranties, expressed or implied, which extend beyond this
description. PathnSitu is not liable for property damage, personal injury, or
economic loss caused by this product.
Bibliography:
1. Ohtsubo M, et al.: Mol Cell Biol1995, 15:2612-2624
2. Spruck C, et al.: Cancer Res 2006, 66:7355-7360
3. Keyomarsi K, et al.: N Engl J Med 2002, 347:1566-1575
4. Del Pizzo JJ, et al.: Am J Pathol1999, 155:1129-1136
5. Li JQ, et al.: Hum Pathol2001, 32:945-953
6. Nakayama N, et al.: Cancer 2010, 116:2621-2634
7. Shaye A, et al.: Breast Cancer Res Treat 2009, 115:651-659
8. Scaltriti M, et al.: ProcNatlAcadSci U S A 2011, 108:3761-3766
Cyclin E1, EP126 antibody has been created by Epitomics Inc., using Epitomics’ proprietary rabbit
monoclonal antibody technology covered under Patent No.’s 5,675,063 and 7,402,409.
US Office: 538, Selby Lane, Livermore, CA- 94551 USA, Ph: +1 925-218-6939
Corporate Office:CDC Towers, 3rd Floor, B-Block, Plot 10/8, Nacharam IDA, Road #5, Nacharam, Hyderabad-76, India.
Phone: 040-27015544/33,Fax:040-2701 5544 ,
E-Mail:[email protected]: www.pathnsitu.com