Download Specification sheet

Rev: A
Release Date: 07/18/2014
IVD
Calcitonin(EP92)
Clone
EP92
Source
Rabbit Monoclonal
Cat#
PR007-6ml RTU
PR007-3ml RTU
CR007-0.5ml Conc
CR007-0.1ml Conc
HAR007-6ml RTU
HAR007-3ml RTU
Intended Use:
Regulatory
IVD
This antibody is intended for use to qualitatively
Status
identify Calcitonin by light microscopy in formalin
fixed, paraffin embedded tissue sections using
immunohistochemical detection methodology. Interpretation of any positive or negative staining must be
complemented with the evaluation of proper controls and must be made within the context of the patient’s
clinical history and other diagnostic tests. A qualified pathologist must perform evaluation of the test.
Summary and Explanation:
Calcitonin is a 32-amino acid polypeptide hormone that is commonly expressed in the parafollicular C cells in
the thyroid gland. Calcitonin is a potent plasma calcium-lowering peptide; it decreases the level of calcium and
phosphate in blood by promoting the incorporation of these ions in bones. Calcitonin antibody is useful for the
identification of C cell hyperplasia and medullary thyroid carcinomas.
Immunogen
A synthetic peptide corresponding to residues in human Calcitonin protein
Isotype:
Rabbit IgG
Reagent Provided:
Concentrated format: Antibody to Calcitonin is affinity purified and diluted in antibody
diluent, with 1% bovine serum albumin (BSA) and 0.05% sodium azide
(NaN3). Recommended dilutions: 1:50 – 1:100.
The antibody dilution and protocol may vary depending on the specimen
preparation and specific application. Optimal conditions should be
determined by individual laboratory.
Pre-diluted format: PathnSitu ready to use antibodies are pre tittered to optimal staining
conditions. Further dilution may loose the activity and may yield to sub
optimal staining.
US Office: 538, Selby Lane, Livermore, CA- 94551 USA, Ph: +1 925-218-6939
Corporate Office:CDC Towers, 3rd Floor, B-Block, Plot 10/8, Nacharam IDA, Road #5, Nacharam, Hyderabad-76, India.
Phone: 040-27015544/33,Fax:040-2701 5544 ,
E-Mail:[email protected]: www.pathnsitu.com
Storage Recommendations: Store at 2-8°C. Donot use after expiration date provided on the vial.
Staining Recommendations:
Antigen Retrieval Solution: Use TRIS-EDTA (PathnSitu cat # PS009) as antigen retrieval solution
Heat Retrieval Method: Retrieve sections under steam pressure for 15
min using PathnSitu’s MERS (Multi Epitope Retrieval System) then allow
solution to cool for 10 minutes then transfer tissue sections/slides to
distilled water.
Primary Antibody:
Cover the tissue sections with primary antibody and incubate for 30
min at room temperature when used PathnSitu PolyExcel Detection
System.
Detection System:
Refer to PathnSitu PolyExcel detection system protocol or manufacturer’s
detection kit staining protocol when used other vendor detection system.
Cellular Localization:
Cytoplasm
Positive Control:
Thyroid or Medullary Carcinoma
Troubleshooting:
Follow the antibody specific protocol recommendations according to data sheet
provided. If unusual results occur, contact PathnSitu Technical Support at 0402701 5544 or [email protected].
Limitations and Warranty: There are no warranties, expressed or implied, which extend beyond this
description. PathnSitu is not liable for property damage, personal injury, or
economic loss caused by this product.
Bibliography:
1. Copp DH, et al.: Endocrinology 1962, 70:638-649
2. Uribe M, et al.: Am J SurgPathol1985, 9:577-594
3. DeLellis RA, et al.: Am J ClinPathol1978, 70:587-594
Calcitonin, EP92 antibody has been created by Epitomics Inc., using Epitomics’ proprietary
rabbit monoclonal antibody technology covered under Patent No.’s 5,675,063 and 7,402,409.
US Office: 538, Selby Lane, Livermore, CA- 94551 USA, Ph: +1 925-218-6939
Corporate Office:CDC Towers, 3rd Floor, B-Block, Plot 10/8, Nacharam IDA, Road #5, Nacharam, Hyderabad-76, India.
Phone: 040-27015544/33,Fax:040-2701 5544 ,
E-Mail:[email protected]: www.pathnsitu.com