Download Specification sheet

Rev: A
Release Date: 30/06/2014
IVD
CK5 & CK14
Clone
EP24 & EP61
Source
Rabbit Monoclonal
Cat #
PR031-6ml RTU
PR031-3ml RTU
Regulatory
Status
IVD
Intended Use:
This antibody is intended for use to qualitatively identify Cytokeratin 5 & 14 antigen by light microscopy in
formalin fixed, paraffin embedded tissue sections using immunohistochemical detection methodology.
Interpretation of any positive or negative staining must be complemented with the evaluation of proper controls
and must be made within the context of the patient’s clinical history and other diagnostic tests. A qualified
pathologist must perform evaluation of the test.
Summary and Explanation:
Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. CK5 is a type II
cytokeratin. Loss-of-function mutations in the keratin 5 gene (KRT5) affected family members and six unrelated
patients with Dowling-Degos disease (DDD), an autosomal dominant genodermatosis. This suggests a crucial
role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear
anchorage.
CK5 labels myoepithelial cells of breast and prostate basal cells. CK5 and calretinin have been useful in
different studies as immunohistochemical markers suggesting mesothelioma, and their expression is analyzed
for the histological differentiation of adenocarcinomas, especially when facing metastatic tumors of unknown
origin.
Cytokeratin 14 (CK14) is a 50-kDa keratin expressed in abundance in stratified epithelial, epidermal, basal,
mesothelial
, and myoepithelial cells in various tissues including breast and prostate. The CK14 antibody is
helpful in the identification of breast cancer with a basal phenotype. It has been reported that Cytokeratin 5/14positive breast cancers are true basal phenotype confined to BRCA1 tumors. Along with p63 and CK5, the
CK14 antibody has been a useful marker for cells with basal, squamous and myoepithelial differentiation.
Isotype:
Rabbit IgG
Immunogen: CK5: A synthetic peptide corresponding to residues near the C-terminus of human CK-5
protein
CK14: A synthetic peptide corresponding to residues near the C-terminus of human
Cytokeratin 14 protein
US Office: 538, Selby Lane, Livermore, CA- 94551 USA, Ph: +1 925-218-6939
Corporate Office: CDC Towers, 3rd Floor, B-Block, Plot 10/8, Nacharam IDA, Road #5, Nacharam, Hyderabad-76, India.
Phone: 040-27015544/33,Fax:040-2701 5544 ,
E-Mail: [email protected] Web: www.pathnsitu.com
Reagent Provided:
Pre-diluted format: PathnSitu ready to use antibodies are pre tittered to optimal staining
conditions. Further dilution may loose the activity and may yield to sub
optimal staining.
Storage Recommendations: Store at 2°-8°C. Do not use after expiration date provided on the vial.
Staining Recommendations:
Antigen Retrieval Solution: Use Citrate (PathnSitu Cat # PS007) as antigen retrieval solution
Heat Retrieval Method: Retrieve sections under steam pressure for 15
min using PathnSitu’s MERS (Multi Epitope Retrieval System) then allow
solution to cool for 10 minutes then transfer tissue sections/slides to
distilled water.
Primary Antibody:
Cover the tissue sections with primary antibody and incubate for 30
min at room temperature when used PathnSitu PolyExcel Detection
System.
Detection System:
Refer to PathnSitu PolyExcel detection system protocol or manufacturer’s
detection kit staining protocol when used other vendor detection system.
Cellular Localization:
Cytoplasm
Positive Control:
Prostate
Troubleshooting:
Follow the antibody specific protocol recommendations according to data sheet
provided. If unusual results occur, contact PathnSitu Technical Support at 0402701 5544 or [email protected].
Limitations and Warranty: There are no warranties, expressed or implied, which extend beyond this
description. PathnSitu is not liable for property damage, personal injury, or
economic loss caused by this product.
Bibliography:
1. Lersch R, et al.: Mol Cell Biol 1989, 9:3685-3697
2. Cid Mouteira P, et al.: Actas Urol Esp 2006, 30:367-371
3. Betz RC, et al.: Am J Hum Genet 2006, 78:510-519
4. Zhou M, et al.: Am J Surg Pathol 2003, 27:365-371
5. Weinstein MH, et al.: Mod Pathol 2002, 15:1302-1308
6. Ali TZ, et al.: Am J Surg Pathol 2008, 32:1890-1895
7. Dabbs DJ. Diagnostic Immunohistochemistry 2010; Churchill Livingstone
CK5&14, EP24 & EP61 antibody has been created by Epitomics Inc., using Epitomics’ proprietary
rabbit monoclonal antibody technology covered under Patent No.’s 5,675,063 and 7,402,409.
US Office: 538, Selby Lane, Livermore, CA- 94551 USA, Ph: +1 925-218-6939
Corporate Office: CDC Towers, 3rd Floor, B-Block, Plot 10/8, Nacharam IDA, Road #5, Nacharam, Hyderabad-76, India.
Phone: 040-27015544/33,Fax:040-2701 5544 ,
E-Mail: [email protected] Web: www.pathnsitu.com