Download Anti-Infectious Salmon Anaemia Virus (ISAV) Mouse Monoclonal

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Anti-Infectious Salmon Anaemia Virus (ISAV) Mouse
Monoclonal Antibody – Supernatant
CLF015
LOT: F1501
DESCRIPTION: Cedarlane's CLF015 antibody recognizes the Infectious Salmon Anaemia Virus (ISAV).
Applications for this antibody include ELISA, Immunofluorescent microscopy, and Immunohistochemisty. In both
the indirect immunofluorescent antibody test and the Immunoperoxidase staining of ISAV-infected cells, CLF015
targeted a viral epitope expressed in the perinuclear region of the cells. This antibody does not neutralize virus in
cell culture, and does not react with ISAV in Western Blots.
PRESENTATION: 0.5 ml Integra Bioreactor concentrated mAb tissue culture supernatant in D-MEM (20% FBS),
0.1% Sodium Azide
STORAGE: Store at +4 to -20°C. Aliquot and freeze the unused portion in volumes appropriate for single use (to
avoid repeated freezing and thawing). If slight turbidity appears, clarify by centrifugation before use.
SPECIFICATIONS:
Clone: 4A11
Specificity: This Mouse Monoclonal antibody (mAb) recognizes ISAV isolates.
Ig class: Mouse IgG1
CONCENTRATION: Approximately 1 mg/ml specific antibody
WORKING DILUTIONS: 1/400 – 1/800 by ELISA
Continued Overleaf…
STAINING:
Immunoperoxidase staining with mAb 4A11 against ISAV-infected TO cells, 4 days post-infection with Norwegian
ISAV isolate 810/9/99. Note localization of the ISAV protein in what appears to be trans-Golgi network (Courtesy
of Dr. Fred Kibenge , Atlantic Veterinary Collage.)
REFERENCES:
Kibenge, F.S.B., Joseph, T., Beecroft, R., Qian, B., and Otto, T. 2003. Generation of monoclonal antibodies to
infectious salmon anaemia virus antigens. 20th Annual Meeting of the Aquaculture Association of Canada,
Victoria, B.C., October 29-November 1, 2003.
FOR RESEARCH USE ONLY
JCr 9/25/06