Download Specification sheet

Rev: A
Release Date: 09/15/2015
IVD
INI-1 (25)
Intended Use:
Clone
25
Source
Mouse Monoclonal
Cat #
PM202-6ml RTU
PM202-3ml RTU
CM202-0.1ml Conc
CM202-0.5ml Conc
HAM202-6ml RTU
HAM202-3ml RTU
This antibody is intended for use to qualitatively
Regulatory
IVD
identify INI-1 antigen by light microscopy in formalin Status
fixed, paraffin embedded tissue sections using
immunohistochemical detection methodology. Interpretation of any positive or negative staining must be
complemented with the evaluation of proper controls and must be made within the context of the patient’s
clinical history and other diagnostic tests. A qualified pathologist must perform evaluation of the test.
Summary and Explanation:
The INI-1 gene, which encodes a functionally uncharacterized protein component of the hSWI/SNF chromatin
remodeling complex, is often mutated or deleted in malignant rhabdoid tumor (MRT). Two isoforms of INI-1,
that differ by the variable inclusion of amino acids, potentially are produced by differential RNA splicing. The
morphology of MRTs can present challenges in differential diagnosis. The overall survival of MRTs relative to
its potential mimics (medulloblastoma, supratenorial primitive neuroectodermal tumors (sPNETs)) is quite low,
and thus differentiation from these other tumors is desirable. Lack of nuclear labeling by anti-INI-1 is
characteristic of MRT. The majority of medulloblastomas and sPNETs are labeled by anti-INI-1. MRTs also
originate from the kidney and soft tissues.
Isotype:
IgG2α
Immunogen:Mouse BAF47 aa.257-359
Reagent Provided:
Concentrated format: Antibody to INI-1 is diluted in antibody diluent, with 1% bovine
serum albumin (BSA) and 0.05% sodium azide (NaN3). Recommended dilutions:
1:50 – 1:100.The antibody dilution and protocol may vary depending on
the specimen preparation and specific application. Optimal conditions should be
determined by individual laboratory.
Pre-diluted format: PathnSitu ready to use antibodies are pre tittered to optimal staining
conditions. Further dilution may loose the activity and may yield to sub
optimal staining.
US Office: 538, Selby Lane, Livermore, CA- 94551 USA, Ph: +1 925-218-6939
Corporate Office:CDC Towers, 3rd Floor, B-Block, Plot 10/8, Nacharam IDA, Road #5, Nacharam, Hyderabad-76, India.
Phone: 040-27015544/33,Fax:040-2701 5544 ,
E-Mail:[email protected]: www.pathnsitu.com
Storage Recommendations: Store at 2°-8°C. Do not use after expiration date provided on the vial.
Staining Recommendations:
Antigen Retrieval Solution: Use Tris-EDTABuffer(PathnSitu Cat # PS009) as antigen retrieval
solution Heat Retrieval Method: Retrieve sections under steam pressure
for 15 min using PathnSitu’s MERS (Multi Epitope Retrieval System) then
allow solution to cool for 10 minutes then transfer tissue sections/slides to
distilled water.
Primary Antibody:
Cover the tissue sections with primary antibody and incubate for 30
min at room temperature when used PathnSitu PolyExcel Detection
System.
Detection System:
Refer to PathnSitu PolyExcel detection system protocol or manufacturer’s
detection kit staining protocol when used other vendor detection system.
Cellular Localization:
Nucleus
Positive Control:
Rhabdoid Tumor, Wilms Tumor
Troubleshooting:
Follow the antibody specific protocol recommendations according to data sheet
provided. If unusual results occur, contact PathnSitu Technical Support at 0402701 5544 or [email protected].
Limitations and Warranty: There are no warranties, expressed or implied, which extend beyond this
description. PathnSitu is not liable for property damage, personal injury, or
economic loss caused by this product.
Bibliography:
1. Bruder CE, Dumanski JP, Kedra D. The mouse ortholog of the human
SMARCB1 gene encodes two splice forms. BiochemBiophys Res Commun.
1999; 257(3):886-890.(Biology)
2. Guidi CJ, Sands AT, Zambrowicz BP, et al. Disruption of Ini1 leads to periimplantation lethality and tumorigenesis in mice. Mol Cell Biol. 2001;
21(10):3598-3603. (Biology)
3.Kalpana GV, Marmon S, Wang W, Crabtree GR, Goff SP. Binding and
stimulation of HIV-1 integrase by a human homolog of yeast transcription factor
SNF5. Science. 1994; 266(5193):2002-2006.(Biology)
US Office: 538, Selby Lane, Livermore, CA- 94551 USA, Ph: +1 925-218-6939
Corporate Office:CDC Towers, 3rd Floor, B-Block, Plot 10/8, Nacharam IDA, Road #5, Nacharam, Hyderabad-76, India.
Phone: 040-27015544/33,Fax:040-2701 5544 ,
E-Mail:[email protected]: www.pathnsitu.com