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Rev: A Release Date: 09/15/2015 IVD INI-1 (25) Intended Use: Clone 25 Source Mouse Monoclonal Cat # PM202-6ml RTU PM202-3ml RTU CM202-0.1ml Conc CM202-0.5ml Conc HAM202-6ml RTU HAM202-3ml RTU This antibody is intended for use to qualitatively Regulatory IVD identify INI-1 antigen by light microscopy in formalin Status fixed, paraffin embedded tissue sections using immunohistochemical detection methodology. Interpretation of any positive or negative staining must be complemented with the evaluation of proper controls and must be made within the context of the patient’s clinical history and other diagnostic tests. A qualified pathologist must perform evaluation of the test. Summary and Explanation: The INI-1 gene, which encodes a functionally uncharacterized protein component of the hSWI/SNF chromatin remodeling complex, is often mutated or deleted in malignant rhabdoid tumor (MRT). Two isoforms of INI-1, that differ by the variable inclusion of amino acids, potentially are produced by differential RNA splicing. The morphology of MRTs can present challenges in differential diagnosis. The overall survival of MRTs relative to its potential mimics (medulloblastoma, supratenorial primitive neuroectodermal tumors (sPNETs)) is quite low, and thus differentiation from these other tumors is desirable. Lack of nuclear labeling by anti-INI-1 is characteristic of MRT. The majority of medulloblastomas and sPNETs are labeled by anti-INI-1. MRTs also originate from the kidney and soft tissues. Isotype: IgG2α Immunogen:Mouse BAF47 aa.257-359 Reagent Provided: Concentrated format: Antibody to INI-1 is diluted in antibody diluent, with 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3). Recommended dilutions: 1:50 – 1:100.The antibody dilution and protocol may vary depending on the specimen preparation and specific application. Optimal conditions should be determined by individual laboratory. Pre-diluted format: PathnSitu ready to use antibodies are pre tittered to optimal staining conditions. Further dilution may loose the activity and may yield to sub optimal staining. US Office: 538, Selby Lane, Livermore, CA- 94551 USA, Ph: +1 925-218-6939 Corporate Office:CDC Towers, 3rd Floor, B-Block, Plot 10/8, Nacharam IDA, Road #5, Nacharam, Hyderabad-76, India. Phone: 040-27015544/33,Fax:040-2701 5544 , E-Mail:[email protected]: www.pathnsitu.com Storage Recommendations: Store at 2°-8°C. Do not use after expiration date provided on the vial. Staining Recommendations: Antigen Retrieval Solution: Use Tris-EDTABuffer(PathnSitu Cat # PS009) as antigen retrieval solution Heat Retrieval Method: Retrieve sections under steam pressure for 15 min using PathnSitu’s MERS (Multi Epitope Retrieval System) then allow solution to cool for 10 minutes then transfer tissue sections/slides to distilled water. Primary Antibody: Cover the tissue sections with primary antibody and incubate for 30 min at room temperature when used PathnSitu PolyExcel Detection System. Detection System: Refer to PathnSitu PolyExcel detection system protocol or manufacturer’s detection kit staining protocol when used other vendor detection system. Cellular Localization: Nucleus Positive Control: Rhabdoid Tumor, Wilms Tumor Troubleshooting: Follow the antibody specific protocol recommendations according to data sheet provided. If unusual results occur, contact PathnSitu Technical Support at 0402701 5544 or [email protected]. Limitations and Warranty: There are no warranties, expressed or implied, which extend beyond this description. PathnSitu is not liable for property damage, personal injury, or economic loss caused by this product. Bibliography: 1. Bruder CE, Dumanski JP, Kedra D. The mouse ortholog of the human SMARCB1 gene encodes two splice forms. BiochemBiophys Res Commun. 1999; 257(3):886-890.(Biology) 2. Guidi CJ, Sands AT, Zambrowicz BP, et al. Disruption of Ini1 leads to periimplantation lethality and tumorigenesis in mice. Mol Cell Biol. 2001; 21(10):3598-3603. (Biology) 3.Kalpana GV, Marmon S, Wang W, Crabtree GR, Goff SP. Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5. Science. 1994; 266(5193):2002-2006.(Biology) US Office: 538, Selby Lane, Livermore, CA- 94551 USA, Ph: +1 925-218-6939 Corporate Office:CDC Towers, 3rd Floor, B-Block, Plot 10/8, Nacharam IDA, Road #5, Nacharam, Hyderabad-76, India. Phone: 040-27015544/33,Fax:040-2701 5544 , E-Mail:[email protected]: www.pathnsitu.com