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Rev: A
Release Date: 01/22/2015
IVD
Mammaglobin Cocktail
(304-1A5&31-A5)
Clone
304-1A5&31-A5
Source
Mouse & Rabbit Monoclonal
Cat #
PMR119-6ml RTU
PMR119-3ml RTU
HMR119- 6 ml
HMR119- 3ml
Regulatory
Status
IVD
Intended Use:
This antibody is intended for use to qualitatively identify Mammaglobin Cocktail by light microscopy in formalin
fixed, paraffin embedded tissue sections using immunohistochemical detection methodology. Interpretation of
any positive or negative staining must be complemented with the evaluation of proper controls and must be
made within the context of the patient’s clinical history and other diagnostic tests. A qualified pathologist must
perform evaluation of the test.
Summary and Explanation:
Mammaglobin is a breast-associated glycoprotein distantly related to secretoglobin family that includes human
uteroglobin and lipophilin. Unlike other secrotoglobin family members, mammaglobin mRNA expression is
breast specific, which has been shown to be a very sensitive marker of occult breast cancer cells in sentinel
lymph nodes (Figure 1) and peripheral blood. By paraffin immunohistochemistry, the overall sensitivity of
mammagloblin for breast cancers (Figure 2) was reported about 80%. When combined with other breastrestricted markers such as GCDFP-15, an overall sensitivity of 84% could be achieved. Mammaglobin can play
a contributing role in the identification of primary sites of carcinomas presenting at metastatic sites.
In normal breast tissue, 304-1A5 and 31A5 labels breast ductal and lobular epithelial cells. In tumor cells, it is
reactive with all types of breast adenocarcinoma regardless tumor differentiation and types. Adenocarcinoms
from other organs rarely express mammaglobin.
Immunogen: Recombinant full-length protein
Isotype:
Mouse IgG1/ Rabbit IgG
US Office: 538, Selby Lane, Livermore, CA- 94551 USA, Ph: +1 925-218-6939
Corporate Office:CDC Towers, 3rd Floor, B-Block, Plot 10/8, Nacharam IDA, Road #5, Nacharam, Hyderabad-76, India.
Phone: 040-27015544/33,Fax:040-2701 5544 ,
E-Mail:[email protected]: www.pathnsitu.com
Reagent Provided:
Pre-diluted format: PathnSitu ready to use antibodies are pre tittered to optimal staining
conditions. Further dilution may loose the activity and may yield to sub
optimal staining.
Storage Recommendations: Store at 2°-8°C. Do not use after expiration date provided on the vial.
Staining Recommendations:
Antigen Retrieval Solution: Use Citrate Buffer (PathnSitu Cat # PS007) as antigen retrieval
solution Heat Retrieval Method: Retrieve sections under steam pressure
for 15 min using PathnSitu’s MERS (Multi Epitope Retrieval System) then
allow solution to cool for 10 minutes then transfer tissue sections/slides to
distilled water.
Primary Antibody:
Cover the tissue sections with primary antibody and incubate for 30
min at room temperature when used PathnSitu PolyExcel Detection
System.
Detection System:
Refer to PathnSitu PolyExcel detection system protocol or manufacturer’s
detection kit staining protocol when used other vendor detection system.
Cellular Localization:
Cytoplasm
Positive Control:
Breast
Troubleshooting:
Follow the antibody specific protocol recommendations according to data sheet
provided. If unusual results occur, contact PathnSitu Technical Support at 0402701 5544 or [email protected].
Limitations and Warranty: There are no warranties, expressed or implied, which extend beyond this
description. PathnSitu is not liable for property damage, personal injury, or
economic loss caused by this product.
Bibliography:
1. Fanger GR, Houghton RL, Retter MW, Hendrickson RC, Babcook J, Dillon DC,
Durham MD, Reynolds LD, Johnson JC, Carter D, Fleming TP, Roche PC, Persing
DH, Reed SG. Detection of mammaglobin in the sera of patients with breast cancer.
Tumour Biol 2002;23(4):212-21
2. Grünewald K, Haun M, Fiegl M, Urbanek M, Müller-Holzner E, Massoner A, Riha K,
Propst A, Marth C, Gastl G. Mammaglobin expression in gynecologic malignancies
and malignant effusions detected by nested reverse transcriptase-polymerase chain
reaction.Lab Invest 2002;82(9):1147-53
3. Watson MA, Dintzis S, Darrow CM, Voss LE, DiPersio J, Jensen R, Fleming TP.
Mammaglobin expression in primary, metastatic, and occult breast cancer. Cancer
Res 1999;59:3028-31.
US Office: 538, Selby Lane, Livermore, CA- 94551 USA, Ph: +1 925-218-6939
Corporate Office:CDC Towers, 3rd Floor, B-Block, Plot 10/8, Nacharam IDA, Road #5, Nacharam, Hyderabad-76, India.
Phone: 040-27015544/33,Fax:040-2701 5544 ,
E-Mail:[email protected]: www.pathnsitu.com