Download GEL ELECTROPHORESIS

GEL ELECTROPHORESIS
What is gel electrophoresis?
-It is a procedure used to separate and
analyze DNA fragments at one ends of a
porous gel and by applying an electrical
voltage to the gel. (See Bio book p. 404).
STEPS in GEL ELECTROPHORESIS
1.) DNA is extracted by opening the cells and
separating the DNA from other cell parts.
2.) DNA molecules are cut precisely into smaller
fragments using restriction enzymes. Each
restriction enzyme cuts DNA at a specific
sequence of nucleotide bases. The R.E. will
only cut a DNA sequence of if matches the
sequence precisely.
(Cont.) STEPS in GEL ELECTROPHORESIS
3.) DNA is separated. Fragments of DNA are
separated by use of a gel electrophoresis
apparatus, whereby a mixture of DNA
fragments are placed at one end of a porous
gel, and an electric voltage (current) is
applied. Different fragments of DNA move
different distances, from the neg. electrode to
the pos. electrode.
SAMPLE GEL ELECTROPHORESIS SLIDE