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Topic B2 Building Blocks of Cells Light microscope Instrument that magnifies specimens using light & lenses Cells Basic units of life in which many chemical reactions needed to sustain life (eg respiration) Cell membrane Thin layer that forms a semi permeable barrier around the outside surface of the cytoplasm of the cell. Controls movement of substances in & out of the cell Cytoplasm Liquid gel that make sup most of the body of a cell. Is where many chemical reactions take place Nucleus Contains DNA for making proteins, new cells and organisms. Also controls the reactions of a cell. Mitochondria Site of cellular respiration where glucose is broken down using oxygen to release energy. Needed for reactions in the cell Cell wall Relatively rigid structure that surrounds plant and bacterial cells. Supports the cell & helps it to keep its shape Cellulose Cell walls are made up of tough cellulose which supports the cell & helps it to keep its shape Vacuole Membrane bound space in the cytoplasm filled with cell sap, a store of water & nutrients. Helps support the plant by keeping cells rigid Chloroplasts Organelle in plant cells that contains chlorophyll. Is where photosynthesis takes place Chlorophyll Green substance in chloroplasts that absorbs energy from sunlight Electron microscope Chromosomal DNA Instrument that magnifies specimens using a ben of electrons Plasmid DNA A circle of extra DNA found only in bacterial cells Flagella Whip like protein found on the outside of a bacterial (and other) cells. Beats back & forth for movement Chromosomes Thread like structure found in the nucleus of a cell. Carries the genetic information DNA that makes up the chromosomes of a cell DNA DNA Molecule that makes up genes and chromosomes. Contains the instructions for a cells growth & activity Gene A section of DNA that codes for a specific protein Double helix The spiral structure of a DNA molecule. Produced by two strands joined by complementary base pairs Bases Adenine Pair up in the same way because the molecules have a complementary shape that match. Adenine + Thymine / Guanine + Cytosine A base (chemical found in DNA) that pairs with thymine thymine A base (chemical found in DNA) that pairs with adenine Cytosine A base (chemical found in DNA) that pairs with guanine Guanine A base (chemical found in DNA) that pairs with cytosine Complementary base pairs Bases that pair up in the same way because the molecules have a complementary shape that match. Adenine + Thymine / Guanine + Cytosine Hydrogen bonds Base pairs are joined together by these (weak) hydrogen bonds Genetic Engineering Human Genome Project (H) A project to sequence (order) all of the base pairs of the human genome. Involves Scientists from many different countries working together Genome (H) All the genetic information (DNA) as a list in order of every base Genetic engineering Process of removing a gene from one organism and inserting it into the DNA in a call of another organism Genetically modified Organisms (GMO) Golden rice An organism that has had a gene inserted from another species (eg human insulin into a bacterium) Beta-carotene Substance in the human diet from which the body makes vitamin A Herbicide Biodiversity Genetically engineered rice that produces beta-carotene in the rice grains – turning them a golden yellow colour Chemical that kills plants. Usually used on weeds The variety of species present within a given area Cell division Diploid A cells that has two sets of chromosomes. In humans, almost all celss are diploid – except sperm & egg cells Growth To increase in size, length, mass and cell number Mitosis Division of parent cell that produces genetically identical diploid cells DNA replication When the chromosomes are copied before cell division occurs Daughter cells Cell produced from the division of a parent cell Parent cell A cell that divides to produce daughter cells Asexual reproduction Formation of a new organism without fertilisation. Uses the process of mitosis to create offspring identical to the parent Sexual reproduction Formation of a new organism from the fertilisation of female gamete (egg cell) by a male gamete (sperm cell) This individual is genetically different from its parents Gametes Sex cells (sperm or eggs) produced in the reproductive organs my meiosis Haploid cells A cell that has one set of chromosomes (a gamete) Fertilisation When two gametes fuse Zygote A fertilised egg cell Meiosis Division of a parent cell that produces two genetically different haploid cells Alleles Different versions of the same gene. Eg alleles for eye colour blue or brown Technology & cell division Clones Individual created from a form of asexual reproduction. Produces offspring that are genetically identical to the parent Enucleated (H) To remove the nucleus from a cell Implanted (H) To place the embryo into the uterus of a female animal to develop Uterus (H) The womb of a female organism Surrogate mother (H) A female who is related to the embryo that is implanted in her womb to develop Embryonic stem cells A cell from an early stage of division of an embryo that can produce almost any type of differentiated cell Stem cell An unspecialised cell that can divide to produce more stem cells or different kinds of specialised cells Differentiated Specialise & develop into different types of cell Adult stem cells Stem cells found in differentiated tissue that can produce a few types of differentiated cells Making proteins, mutations & enzymes Genetic code The code produced by the sequence of bases in genetic material (eg DNA) Amino acids A small molecule that is the building block of a protein Protein synthesis The building up of a protein molecule by joining together amino acids Transcription (H) What an strand of mRNA is produced by complementary pairing of bases with one strand of DNA in the nucleus Messenger RNA Molecule formed during DNA transcription that carries the code from the chromosome to the ribosome Uracil (H) A base only found in RNA with replaces the base thymine in DNA Ribosome (H) Small structures in the cytoplasm of a cell. Where mRNA is translated into an amino acid Base triplets / codons (H) Transfer RNA (H) A group of three bases that code for a particular amino acid Polypeptide (H) A chain of amino acids that will form part of a protein Mutation A change in the base sequences of DNA (often as a result to exposure to radiation) Enzymes A protein molecule made by living cells that speeds up the rate of a reaction Catalysts A substance that speeds up the rate of a reaction without being used up in the reaction Digestion The breakdown of large insoluble food molecules into small soluble food molecules Specific Only one (eg one enzyme can catalyse the reaction of a particular substrate molecule) Active site Site on an enzyme molecule that has a specific shape and holds the substrate molecule during a reaction Lock and key model Idea / hypothesis that describes the relationship of substrate to the active site of an enzyme (helps explain how enzymes work) Denatures To break down / change shape (as protein denature with excess heat) A small RNA molecule that transfers the correct amino acid to the ribosome during translation (so the protein it codes for can be made (synthesised))