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Biochemistry 455 Fall 2009
Techniques in Biochemistry &
Biotechnology
Instructor: Gail Grabner
([email protected])
Topics: Protein purification, Dipeptide sequence analysis,
Molecular cloning, ELISA, Lipid analysis
Student comments:
“I took a lot out of this class; almost nothing was repetitive of anything I
had learned in any other lab.”
“I feel that my lab skills improved after taking BIOC 455. I’ve gained
more experience and learned a lot of techniques and assays.”
“The techniques used here are genuinely applicable, particularly for
students already doing research & considering graduate school.”
“Things I learned in BIOC 455 will most definitely help me when I enter
the workforce.”
Techniques in Biochemistry &
Biotechnology : BIOC 455
CREDITS:
4 hours
WHEN: M, W Lecture: 1:00-1:50; Lab: 2:00-4:50
WHERE: Lab in 218 Noyes Lab
INSTRUCTOR: Gail K. Grabner
CONTACT: [email protected]
BIOC 455: Course Content
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Dipeptide analysis (2 lab periods)
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Photometry (1 lab period)
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Kinetic assays of β-galactosidase
Study of carbohydrates (1 lab period)
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Colorimetric quantitation of total protein concentration.
Study of the properties of β-galactosidase (4 lab periods)
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Determination of the amino acid sequence of a dipeptide
Enzymatic and chemical determination of carbohydrate unknowns
Purification of Glutamate Oxaloacetate Transaminase from
pig heart muscle (5 lab periods)
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Three step purification of an active enzyme from a natural source.
BIOC 455: Course Content

Study of the interaction of RNA polymerase subunits. (1 lab
period)
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Study of the properties of aspartate transcarbamylase (1
lab period)
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Determination of the titers of purified sera, unpurified sera, and
monoclonal ascites.
Characterization of Erythrocyte Membranes (2 lab periods)
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Kinetic assays of the allosteric enzyme ATCase
Use of the ELISA in immunology (3 lab periods)
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Affinity pull-down of separately expressed RNA polymerase subunits
from Methanococcus jannaschii.
Extraction of protein and lipid fractions of pig red blood cells.
In vitro transcription/translation (2 lab periods)

Coupled system to produce isotopically labeled protein product
BIOC 455: Student Comments
BIOC 455: Student Comments
BIOC 455: Student Comments