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OIE Collaborating Centre Reports
Activities in 2011
Title of Collaborating Centre:
Address of Collaborating Centre
Food Safety, Diagnosis and Control of
Animal Diseases for Eastern Europe,
Central Asia and Transcaucasia
FSBI The All-Russian State Center for Quality and
standardisation of veterinary drugs and feed (VGNKI)
5, Zvenigorodskoe shosse,
123022, Moscow,
RUSSIA
Tel.:
+7 (499) 253 14 91
Fax:
+ 7(499) 253 14 91
e-mail address:
website:
[email protected]
www.vgnki.ru
Name of Head of Centre
(Responsible Official):
Dr Alexander N. Panin
Name (including Title and
Position) of Head of the
Collaborating Centre (formally
OIE Contact Point):
Dr Alexander N. Panin
Name (including Title and
Position) of writer of this report
(if different from above)
Irina V. Soltynskaya, research assistant
Annual reports of OIE Reference Centres, 2011
1
Food safety, diagnosis and control of animal diseases in Eastern Europe, Central Asia and Transcaucasia
Summary of activities specifically related to the mandate of
OIE Collaborating Centres
1.
Activities as a centre of research, expertise, standardisation and dissemination of techniques
within the remit of the mandate given by the OIE
1a)
Diagnostic researches of biological material from animals and birds
In 2011 year our Center had carried out analyses for identification of causative agents of different bacterial,
fungus, parasitic diseases of laboratory and domestic animals. Pathological material and corpses were
investigated by different laboratory methods. In 2011 year was investigated 4868 different pathological
materials (organs, smears, excrements, urine, blood, milk, sperm) from agricultural, domestic, laboratory
animals and birds, including parenchymal organs – 803, smears and scrapes – 1863, excrements – 1989,
biological liquids (blood, urine, milk, sperm) – 213. Center had carried out 168 patologo-anatomic researches.
In total Center had carried out 7009 different researches.
1b)
106
2608
1326
126
34
External parasites
401
7009
Blood parasites
660
Dysbacteriosis
Antibiotic
resistance
Bacteriological
researches
Patology-anatomic
researches
168
92
Helminths
50
Protozoa
57
4868
Milk/ sperm
Excrements
1989
Blood
Smears
1863
Urine
Organs
803
Antimicotic
resistance
Total:
Micological
researches
Analysis method
Pathological material
754
840
Diagnosis and surveillance of infectious diseases of the animals and birds
In last year our Center had carried out analyses for identification of causative agents of different infectious
diseases of animals and birds (from poultry farms, cattle-ranchs, fur-farms, animal nurseries).
Causative agent / Disease
Test
No. investigated
samples
Results of research
Positive
Negative
Chlamydia species
Chlamydophila psittaci
Mycobacterium tuberculosis/bovis
Mycobacterium avium
Brucella species
Y. enterocolitica
Canine herpesvirus
PBFD
APV
Feline calicivirus
Herpesvirus
FIP
Mycoplasma species
Salmonella species
Canine distemper morbille virus
C. jejuni
Toxoplasma gondii
Infections canine gepatitis virus (adenovirus)
Canine parainfluenza virus
PCR
PCR
PCR
PCR
PCR
PCR
PCR
PCR
PCR
PCR
PCR
PCR
PCR
PCR
PCR
PCR
PCR
PCR
PCR
3117
483
53
105
35
12
76
495
420
1923
3250
2146
4465
780
3710
123
2303
520
65
516
14
0
0
1
0
0
130
30
467
340
324
2563
25
56
6
1842
11
6
2601
469
53
105
34
12
76
365
390
1456
2910
1822
1902
755
3654
117
461
509
59
PPV
PCR
12
0
12
PEDV
PCR
12
0
12
2
Annual reports of OIE Reference Centres, 2011
Food safety, diagnosis and control of animal diseases in Eastern Europe, Central Asia and Transcaucasia
Causative agent / Disease
Test
No. investigated
samples
Results of research
Positive
Parvovirus
Coronavirus
Avian influenza virus A, H5N1 subtype
Rotavirus
FIV
FelV
Salmonella enteritidis
Salmonella typhimurium
Оrnithobacterium rhinotracheale
Pasterella multocida
Mycoplasma synovia
Mycoplasma gallisepticum
Avian infectious encephalomyelitis
Infectious anaemia
Paramyxovirux -II
Leucosis avium A and B
Leucosis avium J
Avian infectious bronchitis
Newcastle disease
Infectious bursal disease
Avian metapneumovirus (Turkey
rhinotracheitis)
Avian reovirus
Avian infectious laryngotracheitis
Avian influenza virus A
Avian adenoviruses serotypes 1 group I
Avian adenoviruses serotypes 4 group I
Avian adenoviruses group III
Negative
PCR
3670
861
2809
PCR
PCR
PCR
PCR
PCR
ELISA
ELISA
ELISA
ELISA
ELISA
ELISA
ELISA
ELISA
HI
ELISA
ELISA
ELISA
ELISA,
HI
ELISA
ELISA
3420
702
113
985
972
1015
1015
667
145
3673
3259
2331
2191
130
233
145
5662
6095
1903
0
9
96
105
50
21
220
0
1185
3075
588
863
0
0
0
4170
4970
1517
702
104
889
867
965
994
447
145
2488
184
1743
1328
130
233
145
1492
1125
6244
3678
5794
1256
450
2422
ELISA
ELISA
ELISA,
HI
AGP
ELISA,
HI
ELISA
3738
1277
170
3150
628
0
588
649
170
90
230
0
35
90
195
220
87
133
The Centre has carried out monitoring for SPF animals nurseries since 2003. In 2011 year 33 of 13 lines
laboratory SPF animals (mice, rats, hamsters) were tested. 165 samples of parenchymatous organs (lungs,
heart, liver, spleen, kidneys) were investigated, bacterial and fungal microflora wasn’t detected. In 2 rats’s lines
(Wistar and CD) Staphylococcus sciuri was isolated from nasal cavity. Staphylococcus auerus, Staphylococcus
epidermidis и Staphylococcus sciuri were isolated from intestinal tract in all investigated lines, Enterobacter
spp – in mice's line B6CBAF1, Ps.aeruginosa – in mice’s line B6Tg and in rats’ line SHR.
Therefore monitoring of microbiological animal status revealed the contamination laboratory animals by
pathogenic and opportunist pathogenic microorganisms on a large scale, consequently it is necessary to
improve conditions of SPF animals maintenance and feed safety control.
1c)
Determination of the intensity of antirabic immunity and the efficiency of domestic
carnivore's vaccination
In connection with absence of pronounced tendency to decreasing the incidence of rabies the prevention
methods must be improved. The control of efficiency of vaccination allows breaking links in the epizootic
chain and prevents the epizootic process evolution. Therefore European Parliament has issued the directive
№998/2003 according to which animals (dog, cat, polecat) imported in EU-countries must be tested for
antirabic antibodies presence.
The antirabic immunity was estimated on the bases of determination the level of virus neutralizing antirabic
antibodies in the serum of animal blood using the FAVN technique.
Annual reports of OIE Reference Centres, 2011
3
Food safety, diagnosis and control of animal diseases in Eastern Europe, Central Asia and Transcaucasia
The Center had been accredited by AFSSA NANCY in 2005 and was included to the list of rabies testing
authorized laboratories located on the net site:
http://ec.europa.eu/food/animal/liveanimals/pets/approval_en.htm#member_states.
In 2011 the Center had confirmed the international accreditation by AFSSA NANCY (France).
In the period since January 2011 till December 2011 the Centre had tested 389 animals (dogs and cats of
different breeds). Among the tested animals 57 animals had the average antibody titer below the 0,5 IU/ml
value that was 14, 65% of total animals quantity and these animals had been vaccinated repeatedly.
1d)
State monitoring the presence of unintended and undesirable substances in imported feeds
and validation of the declared content of individual raw-materials used in the formulation.
The Center is carried out state monitoring the presence of unintended and undesirable substances in imported
feeds and feed additives. Imported feeds are analyzed to exclude adulteration with contamination of ruminant
(cattle and small cattle) tissue; chicken tissue, pork tissue, soybean or maize ingredients.
For prevention of animal prione diseases spreading EU country have interdicted feeds for cattle which
contained bone meal from mammal’s origin. Developed PCR test systems "BIG” and "PZ-KOM" allow to
reveal ruminant (cattle and small cattle) and mink tissues in feeds and prevent spreading of animal prione
diseases in Russia.
In accordance with directive N13-5-1/1239 16.11.2000 “About quality control of fish flour” had issued by
Russian Ministry of Agriculture, the state control of fish flour import had been started in the Centre. PCR test
system “CHIS” for chicken and swine DNA identification has been used to disclose falsification of fish flour
with chicken and pork tissue.
Without furry animal DNA
With furry animal DNA
Without chicken and pork
DNA
43
39
1
2
1
48
2
1
64
0
0
0
28
136
146
46
50
42
46
2
0
2
3
0
1
46
46
2
1
1
0
71
75
0
0
0
0
0
0
34
37
April
May
158
148
49
55
43
51
1
2
3
2
2
0
48
49
1
0
1
0
78
74
0
0
0
0
0
0
39
37
June
151
53
51
1
1
0
50
2
2
77
0
0
0
31
July
153
52
47
1
3
1
47
0
0
72
0
0
0
29
With chicken DNA
With bovine DNA
124
Without GMO
January
registered
With ruminant DNA
Without ruminant DNA
Furry animal
DNA
not registered
GM maize
DNA was
detected
Chicken/por
k DNA
registered
GM soy
DNA was
detected
Ruminant DNA
not registered
with GMO (35S-promoter,
NOS terminator)
GMO DNA
February
March
period
4
Number of investigated samples
In accordance with the directive N 26 1801.2002 issued in Russia all imported feeds must tested on GMO
presence. Feeds which contain registered in Russia transgenic line are subjected to obligatory state registration.
The Center are authorized for carrying out state control of feeds (GMO detection; identification of the specific
transgenic events; quantitative determination of the relative content of GM soybean/maize DNA to total
soybean/maize DNA) and pre-registration trial of feeds contained GMO. In 2005 FSI VGNKI had been
assessed and certified by “UKAS” as meeting the requirements of ISO 9001:2000 for the activities “Qualitative
and quantitative determination and identification of genetically modified organisms (GMO) in agricultural
products, feedstuff, feed additives and food; exclusion of products adulteration”. In 2011 the Center confirmed
the certificate of accordance with international standard ISO 9001:2000. In 2011 the Center took part in
FEPAS GMO detection researches for the purpose of accreditation according the requirements of ISO 17025.
Annual reports of OIE Reference Centres, 2011
Food safety, diagnosis and control of animal diseases in Eastern Europe, Central Asia and Transcaucasia
August
150
47
42
2
3
0
47
1
1
68
0
0
0
35
September
October
157
147
48
48
44
41
1
1
2
4
1
2
55
48
2
0
1
0
69
73
0
0
0
0
0
0
38
36
November
142
46
42
0
3
1
50
1
1
75
0
0
0
34
December
131
44
42
0
2
0
46
0
0
70
0
0
0
35
TOTAL
1743
581
560
12
30
9
580
12
8
866
0
0
0
413
1e)
Monitoring the presence of unintended and undesirable substances in foods. Food safety
control.
The Center has carried out monitoring the presence of unintended and undesirable substances in food.
In 2011 the Center had tested 2191 samples of foodstuff (foods and farm products, including meat, poultry,
milk, eggs, honey, fish products and other food products) from 47 countries.
As result, 1008 xenobiotics were identified in 742 samples. From them, antibiotics of the tetracycline group
were detected in 720 investigated samples (33,9%) coccidiostatics were detected in 138 investigated samples
(13,7%), nitrofuran metabolites – in 57 (5,7%) investigated samples. Toxic elements – 30 (3,0%), amphenicols
were detected in 17 (1,7%) investigated samples, quinolons were detected in 13 (1,3%) investigated samples.
Sulfamides, nitroimidasols, antgelmintiks, triphenylmethane dyes and penicillins were determined less than
one percent.
In 2011 580 samples of foodstuff have been analyzed for the presence of unintended microorganisms (Listeria
monocyt., Salmonella sp., Staph.gallinarum, Staph.aureus, Candida, E. сoli, Yersinia enteroc., Pseudomonas
aeruginosa, Serratia marcescens and etc.), from which 181 sample (31,2 %) was rejected. Salmonella sp. was
detected in 139 investigated samples (80,8%), Listeria monocytogenes - in 4 samples (2,3%), Coliforms were
detected in 12 investigated samples (6,9%), yeast and mould were detected in 10 samples (5,8%). In 7
investigated samples (4,1%) the Quantity of Mesophilic Aerobic and Facultative Anaerobic Microorganisms
(QMAFAnM) was increased. Also have been recognized by unsuitable through organoleptic characteristics - 3
investigated samples (1,8 %), through presence of lactic acid microorganisms - 1 sample (0,6%) and through
indicators of industrial sterility of group G - 1 sample (0,6 %).
Number of investigated samples included chickens (464 carcasses) of the domestic production, which have
been analyzed for detection the presence of Salmonella in accordance with “The project to assessing the
presence of salmonella in raw poultry meat”, developed by the Society of Consumer Protection of Russian
Federation. Samples were taken at different points of retail trade. Analysis for the presence of salmonella was
carried out in accordance with the methods recommended by WHO, with the use of swabs from whole
carcasses of birds. Pathogenic salmonella was isolated from 136 samples.
In the period since January 2011 till December 2011 the Centre had tested 16 samples of feed (feeding meal,
mill cakes, nutrient supplements, soybean shrots and etc.), from which 9 sample (56,3 %) was rejected. In 6
samples (66,7%) the Salmonella spp. was detected. Coliforms were detected in 2 investigated samples (22,2%).
In 1 investigated samples (11,1%) the Quantity of Mesophilic Aerobic and Facultative Anaerobic Microorganisms
(QMAFAnM) was increased.
1f)
Research
Our efforts are directed to development of test-systems based on using the polymerize chain reaction (PCR)
method. Some new PCR test systems had been developed in 2011:
 PCR test system “Gorbusha- keta- nerka” for detection and differentiation of DNA of mitochondrial
genome fishes Oncorhynchus gorbusha, Oncorhynchus keta, Oncorhynchus nerka using Real time PCR
 PCR test system “RRSS” for detection and genotyping of Porcine reproductive-respiratory syndrome virus
by PCR-method;
These test systems were passed the approbation checkout in 2011 and have been approved by Ministry of
Agriculture.
Annual reports of OIE Reference Centres, 2011
5
Food safety, diagnosis and control of animal diseases in Eastern Europe, Central Asia and Transcaucasia
2.
Proposal or development of any procedure that will facilitate harmonization of international
regulations applicable to the surveillance and control of animal diseases, food safety and animal
welfare.
Our efforts are directed to development of test-systems based on using the polymerize chain reaction (PCR) method.
We offer different kits for detection a wide range of the viral and bacterial infections and for determination of
ruminant tissues or genetically modified organisms in feeds. Our test-systems were affirmed by Ministry of
Agriculture of the Russian Federation. PCR-test systems are distributed within the veterinary laboratories of Russia
and in the countries of the Former USSR.
The list of the test systems:
Test-system “GRIPP” for identification and typing of avian influenza virus A by PCR-method (SТО 00494189-00312009)**
Test-system "CHLA-СOM" for diagnostic of animal and avian chlamydiosis by PCR-method (SТО 00494189-00282007)
Test-system "CHLA-PSIT" for identification of C.psittaci chlamydiosis agent by PCR-method (SТО 00494189-00292007)
Test system “CHLA-FEL” for diagnostics of feline chlamydiosis by PCR method (TC 9388-155-00494189-06)*
Test-system "MYC-KOM" for identification of mycoplasmosis agent by PCR-method (SТО 00494189-0024-2007)
Test-system "MYC-SYN" for identification of M.synoviae bird mycoplasmosis agent by PCR-method (SТО 004941890025-2007)
Test-system "MYC-GAL" for identification of M.gallisepticum bird mycoplasmosis agent by PCR-method (SТО
00494189-0023-2007)
Test-system "MTB-KOM" for identification of tuberculosis agent M.bovis and M.tuberculosis by PCR-method (SТО
00494189-0044-2010)
Test-system "MTB-DIFF" for identification and differentiation of tuberculosis agent M.bovis and M.tuberculosis by
PCR-method (SТО 00494189-0038-2009)
Test system “VD” for diagnostics of cattle viral diarrhea using Real time PCR (STO 00494189-0028-2009)
Test-system "SAL-KOM" for identification of salmonella agent by PCR-method (SТО 00494189-0027-2007)
Test-system "BRU-KOM" for diagnostic brucellosis by PCR-method (SТО 00494189-0021-2007)
Test-system "LISTER" for identification of Listeria monocytogenes by PCR-method (SТО 00494189-0045-2010)
Test-system "ROTAVIR" for identification of rotavirus infectious agent by PCR-method (SТО 00494189-0045-2010)
Test-system "CAM-BAK" for identification and indication of C.jejuni campylobacteriosis agent by PCR-method (SТО
00494189-0027-2008)
Test-system "TOX" for identification of Toxoplasma gondii by PCR-method (TC 9388-096-00494189-04)
Test-system "AVIUM" for identification of M.avium tuberculosis agent by PCR-method (TC 9388-125-00494189-04)
Test-system "SYB-DIF" for detection and differentiation of Bac.anthracis agents by PCR method (STO 00494189-00412009)
Test-system "POLI-CHUM" for diagnosis of carnivore plague with PCR method(SТО 00494189-0033-2009)
Test-system "CALICIVIR" for diagnosis of calicivirosis of cats by PCR method(TC 9388-128-00494189-04)
Test-system "RYNOVIR" for diagnosis of rhinotracheitis of cats by PCR method(STO 494189-0040-2009)
Test-system "ABN" for detection of Aleutian mink disease virus by PCR method (SТО 00494189-0030-2009)
Test system “PARATUB” for identification DNA Mycobacterium avium subsp. paratuberculosis by PCR-method (SТО
00494189-0025-2008)
Test system “PSEUDOTUB” for identification DNA Yersinia pseudotuberculosis by PCR (SТО 00494189-0026-2008)
Test-system "PARVOVIR" for detection and identification of canine parvovirus, feline panleukopenia virus, mink
enteritis virus by PCR method (STO 00494189-0035-2009)
Test-system "ENTERCOL" for detection of Y. enterocolitica by PCR method (ТC 9388-127-00494189-04)
Test-system “ADENOVIR” for detection and differentiation of carnivore’s adenovirus infectious by PCR method (TC
9388-141-00494189-05)
Test system “KCHS” for diagnostics of classical swine fever using Real time PCR (SТО 00494189-0029-2009)
Test-system "RHINO-KOR" for identification of cattle rhinotracheites agent by PCR method (STO 00494189-00522011)
Test-system "NECRO-BAC" for identification Fusobacterium necrophorum subsp. necrophorum by PCR method (TC
9388-147-00494189-05)
Test-system “LPS” for detection of pathogenic Leptospira sp. by PCR method (STO 00494189-0036-2009)
Test system “COLI-DIFF” for Е.coli О157:H7 identification and differentiation of Shiga like toxins (stx type I and II ),
entero-hemorrhagic Е.coli by PCR method (TC 9388-156-00494189-06)
6
Annual reports of OIE Reference Centres, 2011
Food safety, diagnosis and control of animal diseases in Eastern Europe, Central Asia and Transcaucasia
Test-system “CORONAVIR” for detection and identification Coronavirus infections of cats and dogs by PCR method
(STO 00494189-0034-2009)
Test system “LEUCOS” for identification of viral leucosis of cattle by PCR method (SТО 00494189-0032-2009)
Test system “LEUKIS” for diagnostics of feline leukemia by real-time PCR (TC 9388-153-00494189-06)
Test system “ACHS” for identification African swine fever virus using Real time PCR (STO 00494189-0037-2009)
Test - system "BIG" for species-specific identification of ruminant tissues by PCR(SТО 00494189-0020-2007)
Test-system "CHIS" for chicken meat and pork identification in feeds by PCR method (TC 9388-123-00494189-04)
Test system "PLANT-SCREEN" for detection of transgenic constructs control elements in soy and maize genome by
PCR method (TC 9388-122-00494189-04)
Test system "UNI-SCREEN" for detection of transgenic constructions control elements in maize genome by PCR
method (TC 9388-121-00494189-04)
Test system " PZ-KOM " for identification of mink DNA in feeds by PCR method (TC 9388-152-00494189-06)
Test system "GENO-KORM-SOY" for quantitative determination of the relative content of genetically modified
soybean DNA to total soybean DNA by real-time PCR (TC 9388-132-00494189-04 )
Test system " VIK " for diagnostics of feline immunodeficiency by real-time PCR (STO 00494189-0013-2007)
Test system " GMS " for identification of GM Soya by real-time PCR (STO 00494189-0012-2007)
Test system " GMK " for identification of GM Maize by real-time PCR (STO 00494189-0011-2007)
Test system " GMS-DIF " for determination of GM soya’s DNA and for identification events of GM soya: 40-3-2,
A5547-127, A2704-12 by real-time PCR (STO 00494189-0016-2007)
Test system " GENO-KORM-MAIZE " for quantitative determination of the relative content of genetically modified
maize DNA to total maize DNA by real-time PCR (TC 9388-133-00494189-04)
Test system “PVS” for identification porcine parvovirus infection using Real time PCR (STO 00494189-0054-2011)
Test system “EDS” for identification porcine epidemic diarrhoea by PCR-method (STO 00494189-0056-2011)
Test system “TGES” for identification RNA virus of swine transmissible gastroenteritis by PCR-method (STO
00494189-0055-2011)
*TC (Technical Conditions) – is a set of normative documents which prescribed to manufacture, sale and use of biopreparation.
**STO (Organisation Standard) – standard, which was affirmed and was accepted by organisation for the perfection production and for
the ensuring of product quality.
In 2011 The FSBI “VGNKI” supplied biopreparations of standard samples of cultures strains of Leptospira (23
strains) to Republic of Belarus, biopreparations of standard samples of cultures strains of Mycobacterium bovis to
Kazakhstan and Uzbekistan.
3.
Networking
a) Maintenance of a network with other OIE Collaborating Centres designated for the same
specialty
None.
b) Should the need arise, maintenance of a network with Collaborating Centres in other disciplines
None.
4.
Placement of expert consultants at the disposal of the OIE
None.
5.
Provision of scientific and technical training, within the remit of the mandate given by the OIE, to
personnel from Member Countries
None.
6.
Organisation of scientific meetings on behalf of the OIE
None.
Annual reports of OIE Reference Centres, 2011
7
Food safety, diagnosis and control of animal diseases in Eastern Europe, Central Asia and Transcaucasia
7.
Coordination of scientific and technical studies in collaboration with other laboratories,
organisations or collaborating centres
None.
8.
Publication and dissemination of any information within the remit of the mandate given by the OIE
that may be useful to Member Countries of the OIE
None.
_______________
8
Annual reports of OIE Reference Centres, 2011