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Transcript
P06 Novel cardioprotective role of connective tissue growth factor in ischemia/reperfusion injury and heart failure Jørgen A. Gravning1, M. Shakil Ahmed1, Vladimir Martinov2, Thomas G. von Lueder1, 3, Gabor Czibik2, Thor Edvardsen1,3, Birthe V. Mikkelsen1, Otto A. Smiseth1, 3, Guro Valen2, Håvard Attramadal1, 3 1 Inst. for Surgical Research and Dept. of Physiology, University of Oslo 3 Dept. of Cardiology, Rikshospitalet-Radiumhospitalet Medical Center, Oslo, Norway 2 Background: The myocardial growth factor CCN2/CTGF - connective tissue growth factor has been shown to be robustly induced in experimental models of heart failure (HF) as well as in human HF. Yet, its role in the pathophysiologic mechanisms of HF is unknown. Methods and Results: To elucidate the role of myocardial CTGF in ischemia/reperfusion injury and HF, transgenic mice with cardiac-restricted overexpression of CTGF were employed. Transgenic CTGF (Tg-CTGF) hearts had slightly lower cardiac mass than that of non-transgenic littermate controls (NLC) (heart weight/tibia length of 4 months old male Tg-CTGF vs. NLC mice; 58.9±2.7 vs. 68.1±1.2 mg/cm, p<0.05). However, cardiac function assessed by LV pressure-volume analysis was essentially unaltered. Global analysis of gene expression by DNA microarray revealed activation of distinct gene programs in Tg-CTGF hearts, including induction of endoplasmatic reticulum stress response genes, antiapoptotic factors, and ECM proteins, suggesting involvement of CTGF in tissue protection. Also, several signalling mediators reported to confer cardioprotection, e.g. GDF-15, Toll-like receptor-4, and HIF-1, were elevated in Tg-CTGF vs. NLC hearts. Langendorff-perfused hearts from Tg-CTGF (n=9) and NLC mice (n=9) were subjected to 40 min of global (no-flow) ischemia and subsequently 60 min of reperfusion. End-point analysis demonstrated markedly diminished infarct size in Tg-CTGF vs. NLC hearts (21.0±3.9 vs. 49.1±6.3 %, p<0.01) and enhanced recovery of LV function (LV developed pressure 81.3±18.6 vs. 40.4±21.7 mmHg, p<0.01). Western blot analysis also revealed increased levels of phosphorylated (activated) Akt/PKB and GSK-3 in Tg-CTGF vs. NLC hearts, demonstrating activation of bona fide cardioprotective signalling pathways. Consistently, Tg-CTGF mice subjected to pressure-overload by abdominal aortic banding displayed less hypertrophy and preserved LV pressure-volume relations compared with banded NLC mice. Conclusion: These data provide novel evidence that CTGF confers cardioprotection by preemptive preconditioning due to reprogramming of gene expression and may act as endogenous survival factor in HF.