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HRAS EGFR KRAS BRAF TP53 CDKN2A MYC ROS1 NOTCH1 PDGFRA ESR1 ALK FGFR1 RHOA KRAS PTEN GNAS JAK2 ABL1 CDK4 RB1 HNF1A TERT VHL ERBB4 GNAQ IDH1 FBXW7 FGFR2 MAP2K1 MDM2 NPM1 AKT1 CSF1R NRAS EZH2 SRC GNA11 FOXL2 MET IDH2 JAK2 FLT3 STK11 ERBB3 FGFR3 PIK3CA RET Solid Tumor Archer™ VariantPlex™ Solid Tumor Panel Comprehensive Targeting The VariantPlex Solid Tumor Panel is a targeted nextgeneration sequencing assay to detect somatic variants in genes frequently associated with solid tumors. The assay allows simultaneous detection of single nucleotide variants (SNVs), insertions and deletions (indels) and copy number variations (CNVs) in over 19,000 COSMIC variants across 67 oncogenes and tumor suppressors. VariantPlex assays use Anchored Multiplex PCR (AMP™) for target enrichment. AMP enables greater recovery of short fragments commonly found in FFPE samples, a more complex library and robust quantitative data. VariantPlex assays provide better informational yield than competing panels, ensuring that you get the most out of your FFPE sample. The panel is optimized for use with the Illumina® sequencing platform. • Purpose-built – designed for high-quality data • from low-input FFPE samples • Fast – easy, lyophilized workflow with • in-line DNA fragmentation • Integrated CNV calling – in addition to SNPs • and indels For Research Use Only. Not for use in diagnostic procedures. Learn more about the VariantPlex Solid Tumor Panel www.archerdx.com/variantplex/solid-tumor Gene SNV/Indel CNV Gene SNV/Indel ABL1 IDH2 AKT1 JAK2 ALK JAK3 APC KDR ATM KIT KRAS MAP2K1 AURKA BRAF CCND1 CNV MDM2 CCNE1 MET CDH1 MLH1 CDK4 MPL CDKN2A MYC CSF1R MYCN CTNNB1 NOTCH1 DDR2 NPM1 EGFR NRAS ERBB2 PDGFRA ERBB3 PIK3CA ERBB4 PIK3R1 ESR1 PTEN EZH2 PTPN11 FBXW7 RB1 FGFR1 RET FGFR2 RHOA FGFR3 ROS1 FLT3 SMAD4 FOXL2 SMARCB1 GNA11 SMO GNAQ SRC GNAS STK11 H3F3A TERT HNF1A TP53 HRAS VHL IDH1 HRAS EGFR KRAS BRAF TP53 CDKN2A MYC ROS1 NOTCH1 PDGFRA ESR1 ALK FGFR1 RHOA KRAS PTEN GNAS JAK2 ABL1 CDK4 RB1 HNF1A TERT VHL ERBB4 GNAQ IDH1 FBXW7 FGFR2 MAP2K1 MDM2 NPM1 AKT1 CSF1R NRAS EZH2 SRC GNA11 FOXL2 MET IDH2 JAK2 FLT3 STK11 ERBB3 FGFR3 PIK3CA RET Superior Data Yield With random start sites, error-correction, deduplication and expert primer design, the VariantPlex Solid Tumor Panel delivers higher complexity libraries than traditional opposing primer-based library preparation methods. VariantPlex libraries directly reflect input material, capturing the most information while correcting for PCR-induced bias. The primers in the panel are designed to avoid blocking known SNVs. Bidirectional priming and intronic primer placement covers unexpected SNVs while maximizing exon coverage. Sequencing data from VariantPlex library showing deduplicated reads spanning EGFR exon 21. Libraries were prepared from adipose, adrenal, colon and lung gDNA using and sequenced to a depth of 1.87M reads. Accurate CNV Detection AMP chemistry enables detection of copy number gains and losses. Molecular barcodes that label individual input molecules prior to amplification provide unbiased quantitative data. Archer Analysis bioinformatics software then reports relevant CNVs showing copy number gains and losses along with statistical significance. Greater Recovery of Short FFPE Fragments AMP chemistry produces information-rich libraries from short fragments that would otherwise drop out of amplicon-based libraries. In cases of low tumor cellularity and poor sample quality, the loss of shorter fragments could result in false-negative reporting. AMP captures and uniquely tags input molecules of all size, resulting in more complex libraries and more confident calls. archerdx.com Illumina® is a registered trademark of Illumina, Inc. PN-MKT-0031 REV A
