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COVENANT UNIVERSITY COLLEGE OF SCIENCE AND TECHNOLOGY DEPARTMENT OF BIOLOGICAL SCIENCES MICROBIOLOGY PROGRAMME COURSE COMPACT COURSE CODE: MCB 311 COURSE TITLE: MICROBIAL PHYSIOLOGY AND METABOLISM UNITS: 3 SEMESTER/ SESSION: ALPHA SEMESTER 2014/2015 COURSE LECTURERS: Dr. A.A. Ajayi and Miss M.O. Adedeji COURSE CO-ORDINATOR: Dr. A.A. Ajayi TIME: THURSDAYS 8-10AM LOCATION: HALL 107 A. B. BRIEF OVERVIEW OF COURSE: Microbial nutrition and growth. Generation of energy for metabolism, transport system, thermodynamics and enzyme- catalyzed reactions. Fermentation and biosynthesis. COURSE OBJECTIVE/ GOAL: At the end of the course students are expected to: Classify microorganisms based on nutrient requirements. Formulate growth media from the knowledge of microbial growth Requirements Apply the principle of thermodynamics in microbial growth and metabolism Know the role of enzyme in biosynthesis Explain the energy cycle and why energy is essential in biosynthesis Explore the application of microbial metabolism in pharmaceuticals, food, environment and fermentation based industries C. METHOD OF LECTURE DELIVERY/ TEACHING AID: Guided instruction. Interactive classroom session. Multimedia Laboratory session. D. COURSE OUTLINE Module 1 Microbial Nutrition and growth Week 1 Week 2 Week 3 Module 2 Week 4 Week 5 Week 6 Nutritional requirements of microorganisms and growth factors. Transport of nutrients in microorganisms based on nutrient requirement Microbial growth and conditions necessary for growth Generation of Energy An overview of metabolisms, the break down of glucose to Pyruvate Tricarboxylic acid cycle, electron transport and oxidative phosphorylation. Fermentation and Biosynthesis Dr. A.A. Ajayi Week 7 Module 3 Mid semester Test Energy and enzymes Week 8 The energy cycle and the laws of thermodynamics. How free energy is used in reaction. The role of ATP in metabolism Week 9 Oxidation reduction reaction and electron carriers Week 10 Enzymes- Structure and classification mechanisms of enzymes reaction Factors affecting enzymes activities Miss. M.O. Adedeji Week 11 Week 12 Week 13 Revision Examination week E. STRUCTURE OF THE PROGRAMME/ METHOD OF READING 1. Continuous Assessment 30 Marks - Class assignments 10 marks - Mid semester Test 20 marks 2. Semester Examination 70 Marks F. GROUND RULES AND REGULATIONS Student must make at least 80% attendance in lecture and laboratory session There must be no eating or drinking in classrooms or laboratory Student must participate actively when placed to work in groups. All assignment must be submitted within the stipulated time. G. TOPICS FOR TERM PAPERS/ ASSIGNMENTS/ STUDENTS ACTIVITIES Factors affecting enzyme activity The growth of unicellular bacteria in a liquid medium H. ALIGNMENT WITH UNIVERSITY VISION AND GOALS Training in Microbial physiology will give students an overview of what is all about and it will equip them for good performance in other courses in microbiology as they move on in their pursuit of a degree in microbiology. I. INDUSTRIAL RELEVANCE - A well-grounded knowledge of the basics of Microbial physiology is a prerequisite for leadership position in pharmaceuticals, food companies, oil companies, hospitals, diagnostic laboratory and research institutes. J. RECOMMENDED READING - Microbiology: Michael j. Pelczar jr. Ecs Chan, Noel R, Krieg - Enzymes: Dixon and Webb - Principles of Biochemistry: Geoffrey L. Zubay, William W. Parson, Dennis E. Vance