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What is science and how is it done? • What is science? - Science is the systematic investigation of phenomena • How is it carried out? - The SCIENTIFIC METHOD is the systematic process by which such investigations are conducted Scientific Method • • • • Make an observation Ask questions about causes Develop testable hypotheses Make predictions based on hypothesis • Perform experiments to test hypothesis We need repeated observations…replicates! Select 1 marble = blue Assumption: all marbles are blue Sampling error can result from too few observations! Select 10 marbles - 3 marbles blue Assumption: 30% of marbles are blue A Case Study in Scientific Inquiry: Investigating Mimicry in Snake Populations Observation: 1. Dangerous creatures have warning coloration 2. Benign creatures mimic the dangerous Question: Why? Hypothesis: Mimicry evolved in harmless species as an evolutionary adaptation that reduces their chances of being eaten (Henry Bates) Fig. 1-25 Scarlet kingsnake (nonpoisonous) Key Range of scarlet kingsnake only Overlapping ranges of scarlet kingsnake and eastern coral snake North Carolina South Carolina Eastern coral snake (poisonous) Scarlet kingsnake (nonpoisonous) Field Experiments with Artificial Snakes • Experimental Design – fake snakes – An experimental group resembling kingsnakes – A control group resembling plain brown snakes • Equal numbers of both types were placed at field sites, including areas without poisonous coral snakes • After four weeks, the scientists retrieved the artificial snakes and counted bite or claw marks Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings A Case Study in Scientific Inquiry: Investigating Mimicry in Snake Populations Hypothesis: Mimicry evolved in harmless species as an evolutionary adaptation that reduces their chances of being eaten (Henry Bates) Hypotheses for this experiment: H1: The ringed snakes will be attacked less frequently in the geographic region where coral snakes were found Null Hypothesis H0: There will be no difference Fig. 1-26 (a) Artificial kingsnake (b) Brown artificial snake that has been attacked Fig. 1-27 RESULTS 100 84% Percent of total attacks on artificial snakes 83% 80 60 40 20 17% 16% 0 Coral snakes absent Coral snakes present Artificial kingsnakes Brown artificial snakes Fig. 1-25 Scarlet kingsnake (nonpoisonous) Key Range of scarlet kingsnake only Overlapping ranges of scarlet kingsnake and eastern coral snake North Carolina South Carolina Eastern coral snake (poisonous) Scarlet kingsnake (nonpoisonous) A Case Study in Scientific Inquiry: Investigating Mimicry in Snake Populations Hypotheses for this experiment: H1: The ringed snakes will be attacked less frequently in the geographic region where coral snakes were found Null Hypothesis H0: There will be no difference Result: The prediction is upheld. The null hypothesis is not supported, the hypothesis is not rejected. Limitations of Science • repeatable • peer review • Old science is replaced by new science when there is a huge mountain of evidence, not before. • Science cannot support or falsify supernatural explanations, which are outside the bounds of science Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Theories in Science • In the context of science, a theory is: – Broader in scope than a hypothesis – General, and can lead to new testable hypotheses – Supported by a large body of evidence in comparison to a hypothesis – Vetted – EXAMPLE: Evolution – the central unifying theory of Biology and one of the central theories of Geology Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Fig. 22-UN1 Observations Individuals in a population vary in their heritable characteristics. Organisms produce more offspring than the environment can support. Inferences Individuals that are well suited to their environment tend to leave more offspring than other individuals and Over time, favorable traits accumulate in the population. Fig. 22-2 Big new ideas occur from many smaller breakthroughs Linnaeus (classification) Hutton (gradual geologic change) Lamarck (species can change) Malthus (population limits) Cuvier (fossils, extinction) Lyell (modern geology) Darwin (evolution, natural selection) American Revolution 1750 Wallace (evolution, natural selection) French Revolution U.S. Civil War 1800 1900 1850 1795 Hutton proposes his theory of gradualism. 1798 Malthus publishes “Essay on the Principle of Population.” 1809 Lamarck publishes his hypothesis of evolution. 1830 Lyell publishes Principles of Geology. 1831–1836Darwin travels around the world on HMS Beagle. 1837 Darwin begins his notebooks. 1844 Darwin writes essay on descent with modification. 1858 Wallace sends his hypothesis to Darwin. 1859 The Origin of Species is published. Fig. 22-5 GREAT BRITAIN EUROPE NORTH AMERICA ATLANTIC OCEAN The Galápagos Islands AFRICA Pinta Marchena Santiago Fernandina Isabela Genovesa Daphne Islands Pinzón Santa Cruz Florenza Santa Fe Equator SOUTH AMERICA AUSTRALIA PACIFIC OCEAN San Cristobal Cape of Good Hope Tasmania Española Cape Horn Tierra del Fuego New Zealand Fig. 22-6 (a) Cactus-eater (c) Seed-eater (b) Insect-eater Evolution • Evolutionary change is based on the interactions between populations and their environment • A population is the smallest unit that can evolve – Individuals are selected – Populations evolve Fig. 22-12 (a) A flower mantid in Malaysia (b) A stick mantid in Africa Organisms are adapted to their environments – How does this happen? Evolution via Natural Selection Observations • Over production: Organisms produce more offspring than can survive • Limited Resources: There usually isn’t enough to go around. Fig. 22-11 Spore cloud Evolution via Natural Selection Observations • Variation: – Individuals vary extensively (phenotype) • Inheritance of Variation: – Much of this morphological variation is inherited (genotype) Fig. 22-10 Evolution via Natural Selection Inference #1 • Struggle for existence: production of more individuals than can be supported leads to competition for recourses Evolution via Natural Selection Inference #2 • Survival of the best adapted: Individuals with traits that give them an advantage in the struggle for limited resources will survive. These individuals are more likely to leave offspring than individuals that are less fit Evolution via Natural Selection Inference #3 • Accumulation of adaptations:The unequal ability of individuals to survive and reproduce will lead to a gradual change in populations To sum up… 1. Individuals within species vary (phenotypic variation) 2. Some of this variation is heritable (genetic variation) 3. Survival and/or reproduction are non-random (natural selection) The individuals that survive & reproduce the most are those with variations most suited to their environment Hawaiian honeycreepers Evolution is supported by an overwhelming amount of scientific evidence – argued for 250+years • New discoveries continue to fill the gaps identified by Darwin in The Origin of Species • Direct evidence – in the lab and in the field • Fossil Record • Molecular • Homology • Convergent Evolution • Ontogeny Fig. 22-9 DE Terminal bud Cabbage Lateral buds Flower clusters Brussels sprouts Leaves Kale Cauliflower Stem Wild mustard Flowers and stems Broccoli Kohlrabi Fig. 22-3 Layers of deposited sediment Younger stratum with more recent fossils Older stratum with older fossils Fig. 22-16 Fossils (a) Pakicetus (terrestrial) (b) Rhodocetus (predominantly aquatic) Pelvis and hind limb (c) Dorudon (fully aquatic) Pelvis and hind limb (d) Balaena (recent whale ancestor) Molecular evidence includes: Direct sequence similarity Regulatory genes Hox gene 6 Hox gene 7 Hox gene 8 Ubx About 400 mya Drosophila Artemia Fig. 22-17 Morphology: Homologous Structures in mammals Humerus Radius Ulna Carpals Metacarpals Phalanges Human Cat Whale Bat Fig. 22-18 Morphology: Ontogeny Pharyngeal pouches Post-anal tail Chick embryo (LM) Human embryo Fig. 25-19b Morphology: Ontogeny Chimpanzee fetus Chimpanzee adult Human fetus Human adult Fig. 22-20 Sugar glider NORTH AMERICA AUSTRALIA Convergent Evolution Flying squirrel Fig. 25-24 Pigmented cells Pigmented cells (photoreceptors) Epithelium Nerve fibers (a) Patch of pigmented cells Fluid-filled cavity Epithelium Optic nerve Nerve fibers (b) Eyecup Cellular mass (lens) Pigmented layer (retina) (c) Pinhole camera-type eye Optic nerve (d) Eye with primitive lens Cornea Lens Retina Optic nerve (e) Complex camera-type eye Cornea The pace of scientific change