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Photosynthesis and Cellular Respiration Trapping the Sun’s Energy • The process by which plants capture energy from the sun to build carbohydrates is called photosynthesis. – Solar energy converts water and carbon dioxide into chemical energy stored in simple sugars. Trapping the Sun’s Energy • The simple sugar that photosynthesis produces is glucose which the plant uses to store energy. • The equation that represents photosynthesis is: 6CO2 + 6H2O C6H12O6 + 6O2 Solar energy Phases of Photosynthesis • Photosynthesis requires energy from the sun, but the sun is not available 24 hours a day. • Photosynthesis must occur in TWO phases – Light-dependent Reactions (light reactions) • Convert light energy into chemical energy (ATP and NADPH) Phases of Photosynthesis – Light-independent Reactions (dark reactions) • Uses the ATP and NADPH from the light-dependent reactions to build glucose 6CO2 + 6H2O C6H12O6 + 6O2 Solar energy The Role of Chloroplasts and Pigments • Photosynthesis takes place in the chloroplasts • Light-dependent reactions take place in the membranes of the thylakoid disks • Light-independent reactions take place in the stroma The Role of Chloroplasts and Pigments • The thylakoid membranes contain the pigments that can absorb certain wavelengths of sunlight. • The most common pigment in the chloroplasts is chlorophyll. The Role of Chloroplasts and Pigments • Chlorophyll a and b absorb most wavelengths of light except for green. Green is reflected making the plants appear green. • In the fall, plants reabsorb chlorophyll leaving other pigments that reflect other wavelengths of light – making the leaves appear red, yellow, or orange. Light-dependent Reactions • Sunlight strikes the chlorophyll molecules in the thylakoid membrane. • Light energy is transferred to electrons • The electrons become highly energized and are passed down an Electron Transport Chain Light-dependent Reactions • The electrons become highly energized and are passed down an Electron Transport Chain Light-dependent Reactions • The Electron Transport Chain is a series of proteins in the thylakoid membrane • As the electrons are transferred from one protein to another, some energy is released which… Light-dependent Reactions – helps join ADP and Phosphate to form ATP – Pump hydrogen ions into the center of the thylakoid disk to join H+ and NADP+ forming NADPH – ATP and NADPH will be used during the light-independent reactions Light-dependent Reactions • The electrons excited by the light energy that passed down the electron transport chain and left with NADPH need to be replaced so the reaction can happen again. Photolysis Light-dependent Reactions • To replace those electrons, a water molecule is split (photolysis), sending electrons back to the chlorophyll and releasing Oxygen released to atmosphere and Hydrogen ions which remain in the thylakoid – this supplies the oxygen that we breathe. Photolysis Photosynthesis Step 1: Light Reaction – occurs in the thylakoids inside the chloroplast eChlorophyll in the thylakoids e- - h e 1d Water 1a. Light energy is absorbed by the chlorophyll e-+ Electron transport chain O2 + H+ 1b. Electrons raised to higher energy level in the chlorophyll atoms ATP NADP +H= NADP H 1c. Electrons move down the electron transport chain (series of proteins that pass the electrons along). ADP uses energy from electrons to make ATP.. 1d. Photolysis: Water is broken down into oxygen (given off by plant), electrons (replace lost electrons in chlorophyll), and H+ that join with NADP to form NADPH. These electrons return to chlorophyll so the light reaction can happen again. Light-independent Reactions • The second phase of photosynthesis does not require light and is called the Calvin Cycle. • The Calvin Cycle occurs in the stroma of the chloroplast. Light-independent Reactions • The Calvin Cycle uses the ATP and NADPH that was built during the lightdependent reactions Photosynthesis Step 2: Dark Reaction (Calvin Cycle) – Occurs in the STROMA ATP Electrons and ATP from light reaction get dumped into the Calvin Cycle to run it. ATP becomes ADP NADPH Carbon dioxide from the air Calvin Cycle NADPH becomes NADP Energy from ATP and Hydrogen from NADPH combine to form glucose. Calvin Cycle: Series of steps that build up compounds using carbon dioxide from the air. 1 glucose Photosynthesis Equation Light Energy 6CO2 + 6H2O Carbon Dioxide from the air – Used in the Calvin Cycle during the Dark Reactions chlorophyll C6H12O6 + 6O2 Glucose Made in the Calvin Cycle during the Dark Reactions Water Split during Photolysis in the Light Reactions Oxygen Released during Photolysis in the Light Reactions Plants can use this glucose molecule for energy during Cellular Respiration. Plants can also convert this glucose molecule into other organic compounds such as proteins and fats/lipids or other carbohydrates like starch and cellulose What goes in and out 3 Factors that Affect Photosynthesis • Carbon Dioxide (CO2) – Without CO2, the plant would not have one of the raw materials needed in the photosynthesis equation – CO2 is used in the first step of the Calvin Cycle • Temperature – The temperature must be in the appropriate range for the plant in order for photosynthesis to properly occur 3 Factors that Affect Photosynthesis • Intensity of Light – If the intensity of light is lower, the available energy for photosynthesis is lower. • In a greenhouse, if the light source is further away, intensity is lower and less photosynthesis can occur • If light is not available at all, the light-dependent reactions cannot occur (nor can they provide the materials used in the light-independent reactions) Electron transport chain eChlorophyll in the thylakoids e- 1d Water + O2 + H+ e- NADP + H = NADPH ATP ATP ATP becomes ADP Carbon dioxide from the air NADPH Calvin Cycle glucose NADPH becomes NADP Cellular Respiration • Cellular Respiration: Process by which mitochondria break down food molecules to produce ATP in plants and animals Nutrients + oxygen water + ATP + CO2 • Changes the chemical energy in glucose into the chemical energy in ATP Cellular Respiration • There are three stages of Cellular Respiration – Glycolysis • Anaerobic – does not require oxygen – Citric Acid Cycle • Aerobic – does require oxygen – Electron Transport Chain • Aerobic – does require oxygen Glycolysis • Glycolysis: Breaks down glucose into two molecules of pyruvic acid • This reaction uses enzymes and takes place in the cytoplasm of the cell ATP ATP ATP Enzymes + 2 ATP Glucose Pyruvate Pyruvate ATP Glucose breaks down into 2 pyruvate and 4 ATPs (Net gain of 2 ATP) Glycolysis • Produces – 2 pyruvic acid molecules (used in the next step of Cellular Respiration) – 2 ATP molecules (energy the cell can use) ATP ATP ATP Enzymes + 2 ATP Glucose Pyruvate Pyruvate ATP Glucose breaks down into 2 pyruvate and 4 ATPs (Net gain of 2 ATP) Into the Mitochondria… • Before the next step of Cellular Respiration can occur, the pyruvic acid molecules must go into the mitochondria. • The two aerobic reactions are the Citric Acid Cycle (or Krebs Cycle) and the electron transport chain. Pyruvic acid CO2 + water + ATP THE KREB’S CYCLE ATP Citric Acid Cycle (Kreb Cycle) Pyruvate Pyruvate e e- e - NADH + FADH CO2 Electron Transport Chain • Electron Transport Chain uses the electron carriers (NADH and FADH2) to pass electrons down the protein chain and slowly release energy that is used to form ATP and water molecules Electron Transport Cellular Respiration 2 ATP Glucose Pyruvic Acid NADH and FADH Oxygen Glycolysis Citric Acid Cycle Pyruvic Acid CO2 NADH and FADH Water Electron Transport Chain ATPs ATPs Cellular Respiration Equation C6H12O6 + 6O2 Glucose made in photosynthesis by plants or consumed by animals Used in Glycolysis Oxygen from the atmosphere Used in Electron Transport Chain 6CO2 + 6H2O + energy Carbon Dioxide – waste product of the Citric Acid Cycle Water – released from Electron Transport Chain ATP released from Glycolysis, Citric Acid Cycle, and Electron Transport Chain Between 34-36 ATP can be made with this process. This ATP can be used by the cells for cellular metabolism. Fermentation • When oxygen is not available anaerobic respiration, fermentation, can follow glycolysis in order to continue to produce energy. • This is not as efficient as aerobic respiration and produces far fewer ATPs Fermentation • Two types of fermentation: – Lactic acid Fermentation – Alcoholic Fermentation Lactic acid Fermentation • Lactic acid fermentation occurs in muscle cells during strenuous exercise when a lot of energy is required and oxygen is scarce (oxygen debt). Glucose pyruvic acid lactic acid + ATP Lactic acid Fermentation • The lactic acid is transferred from the muscle cells to the liver where it will be converted back into pyruvic acid • The build up of lactic acid in the muscles is what causes them to be fatigued and sore. Alcoholic Fermentation • Yeast and some bacteria cells are capable of alcoholic fermentation during which glucose is broken down to release CO2 and ethyl alcohol Glucose pyruvic acid alcohol + CO2 + ATP Alcoholic Fermentation • The bubbles formed by the CO2 make bread rise • The alcohol released turns grape juice into wine Photosynthesis vs. Cellular Respiration Photosynthesis Stores Energy as glucose Cellular Respiration Releases Energy in glucose Occurs in Living Cells Uses an Electron Transport Chain Occurs in Plant Cells Occurs in Animal Cells Releases Oxygen Releases Carbon Dioxide Creates Energy Neither!