Download 12/101 INVESTIGATOR Name Jeremy P. Brockes Address Ludwig

12/101
INVESTIGATOR
Name
Jeremy P. Brockes
Address Ludwig Institute for Cancer Research, Middlesex Hospital, University College Branch, Courtauld Building, 91 Ridding
House Street, London W1P 8BT, United Kingdom
IMMUNOGEN
Substance
Name
Origin
Chemical Composition
Developmental Stage
IMMUNIZATION PROTOCOL
Donor Animal
Species
Strain
Sex
Organ and tissue
Immunization
Dates immunized
Amount of antigen
Route of immunization
Adjuvant
FUSION
Date
Myeloma cell line
Species
Designation
MONOCLONAL ANTIBODY
Isotype
Specificity
Cell binding
Immunohistology
Antibody competition
Species Specificity
newt skeletal muscle homogenate
Notophthalmus viridescens
adult newt
mouse
BALB/cJ
female
spleen
October, 1982
50-100 micrograms of protein for priming and for several boosts before sacrificing
i.p.
Freund's complete on day 0; Freund's incomplete for boosts
mouse
NS1/SP2
IgG1
skeletal muscle, not smooth or cardiac
newt, Xenopus, rabbit, rat, chicken, mouse, zebrafish
ANTIGEN
in myofibrils, perhaps associated with contractile apparatus
Chemical properties
sensitive to acid alcohol but resistant to aldehydes
Molecular weight
102 kDa (note misprint in JEEM paper)
Characterization
Immunoprecipitation
Immunoblotting
Purification
Amino acid sequence analysis
Functional effects
Immunohistochemistry
probably membrane component of sarcoplasmic reticulum
PUBLICATIONS :
Kintner, C.R., and Brockes, J.P. (1984). Monoclonal antibodies identify blastemal cells derived from dedifferentiating muscle in
newt limb regeneration. Nature 308, 67-69.
Gurdon, J.B., Fairman, S., Mohun, T.J., and Brennan, S. (1985). Activation of muscle-specific actin genes in Xenopus development
by an induction between animal and vegetal cells of a blastula. Cell 41, 913-922.
Kintner, C.R., and Brockes, J.P. (1985). Monoclonal antibodies to the cells of a regenerating limb. J. Embryol. Exp. Morph. 89,
37-55.
Smith, J.C. (1987). A mesoderm-inducing factor is produced by a Xenopus cell line. Development 99, 3-14.
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Griffin, K.J.P., Fekete, D.M., and Carlson, B.M. (1987). A monoclonal antibody stains myogenic cells in regenerating newt muscle.
Development 101, 267-277.
Gurdon, J.B. (1988). A coummunity effect in animal development. Nature 336, 772-774.
Neff, A.W., Malacinski, G.M., and Chung, H.M. (1989). Amphibian (urodele) myotomes display transitory anterior/posterior and
medial/lateral differentiation patterns. Dev. Biol. 132, 529-543.
Young, H.E., Sippel, J., Putnam, L.S., Lucas, P.A., and Morrison, D.C. (1992). Enzyme-linked immuno-culture assay. J. Tiss. Cult.
Meth. 14, 31-36.
Devoto, S.H., Melançon, E., Eisen, J.S., and Westerfield, M. (1996). Identification of separate slow and fast muscle precursor cells in
vivo, prior to somite formation. Development 122, 3371-3380.
Hanken, J., Klymkowsky, M.W., Alley, K.E., and Jennings, D.H. (1997). Jaw muscle development as evidence for embryonic
repattering in direct-developing frogs. Proc. R. Soc. Lond. B 264, 1349-1354.
ACKNOWLEDGMENTS STATEMENT
We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of
the DSHB's products. We suggest that the following statement be used:
“The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was
obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University
of Iowa, Department of Biology, Iowa City, IA 52242.”
Please send copies of all publications resulting from the use of Bank products to:
Developmental Studies Hybridoma Bank
Department of Biology
The University of Iowa
028 Biology Building East
Iowa City, IA 52242