Download Evaluation of Reversine as an anticancer drug in oral cancer

Evaluation of Reversine as an anticancer
drug in oral cancer
Speaker: Wei-Ching Wu
Advisor: Dr. Hau-Ren Chen
Date: 6/04/2010
Betal quid-chewing induced oral cancer is a common disease in South Asia. The
incidence of oral cancer is about 11% of male population in Taiwan, and the mortality
of oral cancer is about 8%. Surgery and radiation are the effective therapies in oral
cancer treatment. In addition, the chemotherapy agents such as 5-Fu and Cisplatin are
used in oral cancer commonly. However, the five-year survival rate is less than 30%
even though the treatments. Therefore, it is imperative to investigate the novel and the
effective drugs in oral cancer treatment.
Reversine is a synthetic purine-like small molecular which is first identified as a
de-differentiation agent in murine myoblast cell lines. Moreover, it has been
demonstrated as an inhibitor of Aurora kinase and which can prohibit the survival of
leukemia cells. Because Aurora kinase has also been reported to overexpress and
contribute to the oral cancer tumorigenesis. In this study, we propose that Reversine
may be a novel anticancer agent in oral cancer. To verify this hypothesis, two oral
squamous cell carcinoma (OSCC) cell lines, OC2 and OCSL, were used to test the
influence of Reversine in cellular variability. Cell counting after Reversine treatment
showed that the IC50 is about 5uM in 24 hours. Moreover, cell cycle analysis and
western blot showed that Reversine might induce cell cycle arrest in G2/M phase. In
addition, apoptosis was also be detected by flow cytometry during Reversine
treatment. Western blot analysis was further used to elucidate that Reversine could
induced caspase-dependent cell death in oral cancer cell lines. Taken together, our
results suggested that Reversine can be a novel anticancer agent in oral cancer
therapy.
Evaluation of Reversine as an anticancer drug in oral cancer
Date: 6/4/2010
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Speaker: Wei-Ching Wu
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Adviser: Dr. Hau-Ren Chan