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Steps of the Cell Cycle â Metaphase: ⢠Normally the shortest phase of mitosis. ⢠The chromosomes line up single file in the center of the cell. â Anaphase: ⢠The spindle fibers separate the centromeres that hold the two copies of each piece of DNA together and pull one half of each chromosome (one copy of the DNA) to each side of the cell. â Telophase: ⢠The DNA uncoils. ⢠The spindle fibers break down and the cetrioles move back to their original location. ⢠Two nuclei form.
