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Download Separation of Carbon Nanotube by Dielectrophoresis
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A Micro-flow Cytometer Based on Dielectrophoretic Particle Focusing Choongho Yu, Li Shi University of Texas at Austin Jody Vykouka, Peter Gascoyne UT MD Anderson Cancer Center Support: UT BME Program /Whitake Foundation Conventional Flow Cytometry Hydrodynamic Focusing Injector Tip Sheath fluid Fluorescence signals Picture Courtesy: Purdue University Focused laser beam Cytometry Laboratories 1. Focus cells in suspension by sheath fluid 2. Illuminate cells in the focused suspension stream 3. Analyze cells by detecting scattered and fluorescence signals Motivation for a Micro-cytometer Conventional User Complex Interface Operated by skilled personnel Size etc. Large, heavy Price Micro (Goals) Easier to operate Small, portable A reservoir is required for the sheath flow medium, and need to be kept free of dust and bacteria Large components unnecessary Eliminate the use of gallons of sheath liquid Expensive Inexpensive •Collaboration: P. Gascoyne, UT MD Anderson Cancer Center Dielectrophoretic (DEP) Particle Focusing E FDEP m1 flow Fringing fields at electrode edges provide DEP levitation forces in direction normal to the electrode plane A Micro-Flow Cytometer based on DEP Focusing Circular electrode Top glass wafer Circular channel Bottom Si wafer • Electrodes focus cells to the center of the channel by negative DEP force • Optical sensors can be fabricated on the bottom Si wafer for cell diagnostics PhD Student: Choongho Yu Electrode Circular channel Proof of Principle
