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Transcript
Genetics EQ: How are traits passed down through generations? Gregor Mendel = Father of Genetics 1857 Worked with garden pea plant Mendel’s Experiments • Peas are a “model system.” • • Small, easy to grow. • Makes them convenient to study. • Help explain how organisms inherit traits • Started with “true breeding” plants • Trait: specific characteristic (e.g., seed color, plant height) of an individual • Hybrid: created from cross of true-breeding individuals Pollination and Fertilization in Plants Plants have the ability to self-pollinate (self-fertilize) because they have both reproductive parts. Male part Female part Cross-Pollination Taking pollen from one plant and using it to fertilize the eggs of another plant Pollen Pea Characteristics of Mendel’s peas Mendel studied these characteristics to observe patterns in traits across generations. Mendel’s Crosses When Mendel crossed plants with contrasting traits, the hybrid offspring showed traits of only one parent. Dominant and Recessive Traits When a yellow pea plant is crossed with a green pea plant, the resulting offspring are yellow. Yellow seed color is to green seed color. Green seed color is to yellow seed color. dominant recessive Principle of Dominance • Some alleles are dominant, some recessive. • An organism with at least one dominant allele will exhibit that trait. • An organism with a recessive allele will exhibit the trait only in the absence of a dominant allele. dominant recessive dominant Genes and Alleles Traits (seed shape, seed color) are determined by genes. •Genes: passed from one generation to the next; genetic code that determines an individual’s characteristics Forms of traits (yellow/green, round/wrinkled) are called alleles. •Alleles: the different forms of a gene. Characteristics such as pod color are determined by genes . Yellow and green pod colors are determined by alleles . The allele for green pods is recessive to the allele for yellow. The P1 and F1 Generation P1 generation True-breeding F1 generation Hybrids Let’s figure out how we can predict the F1 gen from the P1 gen. Monohybrid Cross = One Trait “Height” Tall Pea x Short Pea T = Dominant t = Recessive TT x tt “Homozygous Dominant” “True Breeding” = Homozygous X “Homozygous Recessive” Heterozygous and Homozygous Homozygous: has two identical alleles for a gene Heterozygous: has two different alleles for a gene Which are: homozygous? 1, 4 heterozygous? 2, 3 1 2 3 4 1st LAW OF HEREDITY “Law of Segregation” = – The 2 alleles for each trait must separate when gametes (sperm/egg) are formed. -A parent passes on, at random, only one allele for each trait to each offspring A aa Segregation Random Fertilization The Formation of Gametes Eggs and sperm Fertilization Making a Punnett Square A cross of birds: Beak size (B, b) Combine gamete genotypes Possible gametes Gametes for a parent along one side Generations P1 = Parent Plants -True breed Tall x True breed Short TT x tt F1 = First Generation (children) What’s the probability that the offspring will be tall? ________ Short? _______ Genotype of offspring? ______________ Genotype=genetic makeup of organism Phenotype of offspring? _____________ Phenotype=physical characteristics Law of Probability “Rules of Chance” “Odds” Numerator = winner Denominator = # horses 1/12 20:1 5% 1:12 “odd” 10:1 10% 8% “chance” 2:1 50% Probability? “Chance” of getting a tail? “Chance” of getting snake eyes on two dice? “Chance” of getting a boy? Male: Female XY XX ½ 1:2 odd Boy 1:2 odd Girl 50% chance Survey Large Number 1:1 Alleles: Tall = Dominant (T) Short = Recessive (t) Dominant = Trait Observed Recessive = Trait that disappeared Genotype Phenotype TT = Tall Tt = Tall tt = Short Can’t always know an organisms genotype by looking at its phenotype? Homozygous Dominant Homozygous Recessive tt TT T t Tt Heterozygous Change of colors to follow the parental alleles: F1 Tt x Tt Combination of Letters/Alleles? F2 3 Tall and 1 Short TT Tt Tt tt The F2 Generation – created from a cross of two F1 generation offspring. What proportion of F2 offspring were short? 1/4 What proportion of F2 offspring were tall? 3/4 Dihybrid Crosses: Crossing for 2 characteristics/ traits EXAMPLE •2 TRAITS: Seed Shape and Seed Color •Shape =Round (R) was dominant to Wrinkled (r) •Color = Yellow (Y) was dominant to Green (y) Making a Punnett Square: Two Factors A cross of pea plants: size (T, t) and pod color (G, g) All possible gametes All gametes for a parent along one side Combine gamete genotypes Round Yellow Seeds x Wrinkled Green Seeds Homozygous dominant for both traits _RRYY_(Genotype) Use FOIL to help you figure out the combinations (RR)(YY) F(irst) RY O(uter) RY I(nner) RY L(ast) RY Homozygous recessive for both traits 1. We need to first find all the possible gamete combinations of each parent ry ry ry ry Use FOIL to help you figure out the combinations (rr)(yy) F(irst) ry O(uter) ry I(nner) ry L(ast) ry gametes 2. Place your gametes in a Punnett square. RY (Genotype) ___rryy____ RY RY RY gametes 3. Fill in the Punnett square. ry RY RrYy RY RrYy RY RrYy RY RrYy ry RrYy RrYy RrYy RrYy ry RrYy RrYy RrYy RrYy ry RrYy RrYy RrYy RrYy So the P1 cross of true breeding plants: Round Yellow Seeds P1 Wrinkled Green Seeds rryy RRYY produced F1 RrYy All plants Round Yellow Seeds Now let’s cross two F1 generation plants. 1. We need to first find all the possible gamete combinations of each parent Use FOIL! RrYy RY Ry rY ry RrYy RY Ry rY ry The presence of one specific allele for one trait has no impact on the presence of a specific allele for the second trait. The two pairs of alleles segregate independently of each other. Four classes of gametes (RY, Ry, rY, and ry) would be produced in equal amounts. RY Ry RrYy rY ry 2. Place your gametes in a Punnett square. RY RY Ry rY ry Ry rY RY Ry rY ry ry RY Ry rY ry RrYy Round Yellow Round Green RrYy Wrinkled Yellow Wrinkled Green ND 2 LAW OF HEREDITY • Law of Independent Assortment = genes for different traits are inherited “independently” of each other. •RrYy = the R and r will separate as well as the Y and y. •Alleles can recombine in 4 different ways.