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Name __________________________ Date _______ Period _______ Lab # _________ - Human Heart Lab Heart muscle tissue contracts and relaxes to pump blood throughout your body. The blood, carrying oxygen and other materials, moves through the circulatory system which is composed of arteries, capillaries and veins. In this investigation, you will: Follow the pathway of blood through the heart. Determine the amount of oxygen or carbon dioxide contained in blood in each side of the heart. Follow the sequence of events occurring as a heart beats. Measure blood pressure differences in arteries and veins using a heart-blood vessel model. Materials: Plastic bottle 2-hole stopper to fit bottle Metric ruler Glass tube, 3 cm long Glass tube, 18 cm long Glass tube, 16 cm long Procedure: Part A. Heart Anatomy and Blood Flow Study Figure 1 to determine the names and locations of all major blood vessels and heart structures. This diagram is a front view of the heart, which makes the labels indicating left and right sides appear to be reversed. All shaded areas are muscle. Unshaded areas are filled with blood. Complete Table 1 indicating the direction of blood flow. Part B. Condition of Blood as it Flows Through the Heart All vessels bringing blood to the heart’s right side and leaving from the right ventricle contain blood that is deoxygenated. Deoxygenated blood is low in oxygen and high in carbon dioxide. All vessels bringing blood to the heart’s left side and leaving from the left ventricle contain oxygenated blood. Oxygenated blood is high in oxygen and low in carbon dioxide. 1. Blood moves to two organs from the right side of the heart. What are these organs? Complete Table 2 indicating the oxygen content of blood. Use the terms “deoxygenated” and “oxygenated.” Refer to Figure 1 for help. 2. Blood is received from two organs on the left side of the heart. What are these organs? 3a. Describe the condition of blood in all parts of the right side of the heart. 3b. Describe the condition of blood in all parts of the left side of the heart. Aorta – Blood to Body Pulmonary Artery – Blood to Left Lung Superior Vena Cava – Blood from upper body Pulmonary Artery – Blood to Right Lung Pulmonary Vein – Blood from Right Lung Pulmonary Vein – Blood from Left Lung Left Atrium Right Atrium Bicuspid Valve Tricuspid Valve Semilunar Valves Right Ventricle Left Ventricle Inferior Vena Cava – Blood from lower body RIGHT Table 1 – Blood Flow Receives Blood From Left Side 1. 2. FIGURE 1 LEFT Table 2 – Oxygen Content of Blood Chamber or Vessel Oxygenated or Deoxygenated Left Ventricle Right Ventricle Left Atrium Right Side Left Side Right Side 1. 2. Right Atrium Pulmonary Artery Pulmonary Vein Pumps Blood To Superior Vena 1. Cava 2. 1. 2. Inferior Vena Cava Aorta Part C. Heart Pumping Action In order to move blood through the heart, a pumping action must occur. It is the ventricles that aid in the pumping action of the heart. Heart valves keep the blood flowing in one direction as the ventricles squeeze or pump blood through the heart. Examine Figure 2 showing the ventricles relaxed and not pumping blood. This relaxed condition is called diastole. Complete the left column of Table 3 while looking at Figure 2. Examine Figure 3 showing the ventricle sides pushing in and squeezing and pumping blood out of the heart. This pumping action is called systole. Complete the right column of Table 3 by looking at Figure 3. 4. (a) During diastole, are the ventricles filling or being emptied of blood? Table 3 Ventricles In Diastole Systole Relaxed or pumping Bi- and tricuspid valves open or closed? * Blood flowing past bi- and tricuspid valves? Blood flowing into ventricles from atria? Semilunar valves open or closed?* Blood flowing past semilunar valves? Blood flowing out of ventricles into aorta or pulmonary artery? *Valves are open if their tips are not touching. A continuous pattern of diastole and systole allows the heart to pump blood to all parts of the body. The heart relaxes a little and fills with blood, then pumps. It relaxes again while it refills, and then pumps again. You detect this pattern of relaxing and pumping when you feel your pulse. (b) During systole, are the ventricles filling or being emptied of blood? FIGURE 2 (DIASTOLE) FIGURE 3 (SYSTOLE) Part D. Blood Pressure Model Blood is under pressure as the heart pumps it through your body. The amount of pressure, however, varies throughout your body. Blood vessels called arteries have thicker walls and are less flexible. Arteries have blood under high pressure. Other blood vessels, veins, have blood under low pressure because of their thinner, more flexible walls and because of the loss of pressure that occurs as blood passes through the capillaries. Secure a plastic bottle from your teacher. Fill it with water and seal it with the provided rubber stopper and tube assembly. The finished apparatus should look like Figure 4. Note that one of the tubes leading from the stopper is glass while the other is plastic. 5. (a) Which tube represents the flexible blood vessel? (b) Which tube represents the inflexible blood vessel? Position the plastic bottle assembly on a counter. Place a metric ruler next to the bottle. Give the plastic bottle one firm squeeze. Measure the distance (in mm) that the water streams shoot out the ends of the tubes. Record the distances in Table 4 using the Trial 1 row. Repeat the squeezing and measuring four more times. Calculate an average distance for each tube. Table 4 – Experimental Results Glass Tube Plastic Tube Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Average Distance A tube having more flexible sides will have a lower pressure. A tube having less flexible sides will have a greater pressure. The higher the pressure, the farther a stream of water will shoot from a tube. The lower the pressure, the shorter a stream of water will shoot from a tube. 6. (a) Which tube has the longer average stream of water? (b) Which tube has the shorter average stream of water? 7. (a) Which tube has the higher pressure within it? (b) Which tube has the lower pressure within it? 8. (a) Which tube represents an artery? (b) Which tube represents a vein? Analysis (use complete sentences to answer the questions below) 1. Define the following terms: Oxygenated blood _________________________________________________________ Deoxygenated blood _______________________________________________________ Systole _________________________________________________________________ Diastole ________________________________________________________________ 2. Blood is changed from an oxygenated to a deoxygenated condition or vice versa in the circulatory system. Which change occurs in lung capillaries? __________________________ _______________________________________________________________________ Which change occurs in body capillaries? _________________________________________ _______________________________________________________________________ 3. Using Figure 1 as a guide, tell where blood goes when it leaves the: Aorta __________________________________________________________________ Pulmonary artery __________________________________________________________ Right and left atria ________________________________________________________ 4. Using Figure 1 as a guide, tell where blood comes from in each of the following structures: Superior vena cava _________________________________________________________ Inferior vena cava _________________________________________________________ 5. Describe the direction of blood flow through the right side of the heart. Include the names of all blood vessels leading into and out of the right side as well as valves involved in blood flow. Indicate which chambers are in systole and diastole. 6. Describe the direction of blood flow through the left side of the heart in the same way as above. 7. Your heart ejects or pumps about 70 ml of blood into the aorta each time it undergoes systole. (a) How many times in one minute does your heart pump (beat)? ________________________ (b) Calculate the amount of blood pumped by your heart in one minute (show work). 8. (a) Assume that the bi- and tricuspid valves were closed during diastole. What would happen to blood movement? ________________________________________________________ _______________________________________________________________________ (b) Assume that the semilunar valves were closed during systole. What would happen to blood movement? _______________________________________________________________ _______________________________________________________________________ 9. In Part D, what body part was represented by the: (a) Plastic bottle? __________________________________________________________ (b) Water in the bottle? _____________________________________________________ (c) Plastic tube? ___________________________________________________________ (d) Glass tube? ____________________________________________________________ 10. A student observes the following cross section slices of blood vessels under a microscope: A (a) Which vessel is probably an artery? Why? (b) Which vessel is probably a vein? Why?