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SULTAN lORIS EDUCATION UNIVERSITY
UNIVERSITI PENDIOIKAN SULTAN lORIS
COURSE: CELL BIOLOGY
CODE: SBC3013
DATE:
2 B DEC 2015
DURATION: 2 HOURS 30 MINUTES
INSTRUCTIONS
question paper consists of THREE (3) sections: A, B, and C.
Answer ALL questions in Section A and Section B. Answer any TWO (2) questions
1.
This
2.
in Section C.
Section A consists of FOURTY
(40) multiple choice questions. Answer all questions
in Section A in OMR form provided.
Section B consists of THREE (3) structure questions. Answer all questions in
Section B in this question booklet.
Section C consists of THREE (3) essay questions. Answer any TWO (2) questions in
3.
4.
5.
Section C in this
6.
answer
booklet.
Question paper will be collected at the end of the examination.
This 'question paper contains of 19 pages
including
cover
page
"
PROGRA�ME:
YEAR:
___
CLASS GROUP:
_
REGISTRATION NO:
IDENTITY CARD NO
_
:
LECTURER: DR. REMMY KEONG BUN POH
DO NOT OPEN THIS QUESTION PAPER UNTIL YOU ARE TOLD TO DO SO
CONFIDENTIAL
2
SBC3013 Cell Biology
SECTION A
(40 marks)
Instruction: Answer all questions
1.
Which of the
following
ribosomes that
are
molecules is
produced in large numbers by specialized
present in cells?
A. Lipids
B. Starches
c. Proteins
D. Glucose
2.
Which
type of organelle is primarily
involved in the
synthesis of oils, phospholipids,
and steroids?
A. Ribosome
B.
Lysosome
c. Mitochondrion
D. Smooth endoplasmic reticulum
3.
Which structure is the site of the synthesis of
proteins
that may be exported from the
cell?
ER
A.
Rough
B.
Lysosomes
c.
Golgi apparatus
D. Plasmodesmata
[See
next page
3
SBC3013 Cell Biology
4.
The fact that the outer membrane of the nuclear envelope has bound ribosomes
allows
one
to most
reliably
conclude that
_
A. the nuclear envelope is not part of the endomembrane system
B. small vesicles from the Golgi fuse with the nuclear envelope
C. the nuclear envelope is physically continuous with the endoplasmic reticulum
D. at least
some
ribosomes
5.
on
In animal cells,
cellular
proteins that function in the
nuclear envelope
of the
the
hydrolytic
components.
enzymes
Which
of
nuclear
envelope
are made
by
the
packaged to prevent general destruction of
the following organelles functions in this
are
compartmentalization?
A.
Lysosome
B. Chloroplast
C. Peroxisome
D. Central vacuole
6.
cells accumulating
Tay-Sachs disease is a human genetic abnormality that results in
and becoming clogged with very large and complex lipids. Which cellular organelle
must be involved in this condition?
A. Mitochondria
B. The lysosome
7.
C. The
Golgi apparatus
D. The
endoplasmic
Which is
one
reticulum
of the main energy transformers of cells?
A. Vacuole
B.
Lysosome
C. Mitochondrion
D.
Golgi apparatus
[See
next page
4
SBC3013 Cell Biology
8.
Grana, thylakoids,
and stroma
are
all components found in
_
A. vacuoles
8. Iysosomes
C. chloroplasts
D. mitochondria
9.
Which of the
following
contain the 9
2 arrangement of microtubules?
Cilia
I.
Centrioles
II.
III.
A. I
+
Flagella
only
8. I and II
C. I and III
only
only
D. All of above
10. If
an
individual has abnormal microtubules, due to
organs
A.
or
tissues would you
Sperm, larynx,
D. All ducts, such
11. What
are
hereditary condition, in
which
expect dysfunction?
and trachea
8. Microvilli, alveoli, and
C. Limbs, hearts,
a
areas
as
glomeruli
with
a
good deal of contraction
those from
the products of the
salivary
light
or
sebaceous
reactions that
are
glands
subsequently used by the Calvin
cycle?
A. Water and carbon
8. ATP and NADPH
C. Carbon dioxide and RuBP
D. Oxygen and carbon dioxide
[See
next page
5
SBC3013 Cell Biology
12. Where does the Calvin cycle take place?
A.
Thylakoid
membrane
8. Chlorophyll molecule
C. Stroma of the chloroplast
D.
Cytoplasm surrounding the chloroplast
13. Where
are
the molecules of the electron transport chain found in plant cells?
A. Stroma of chloroplasts
8. Matrix of mitochondria
C. Inner membrane of mitochondria
D. Thylakoid membranes of chloroplasts
14.
During photosynthesis,
the release of oxygen is
a
by-product of
_
A. reducing NADP+
8.
splitting
the water molecules
C. the electron transfer system of photosystem I
D. the electron transfer system of photosystem II
15. The
reaction-center
A. there
are
chlorophyll of photosystem I is known
D. this
are
P700
because
700 chlorophyll molecules in the center
8. the plastoquinone reflects
C. there
as
700
light with
a
wavelength of 700
nm
photosystem I components to each chloroplast
pigment is
best at
absorbing light with
a
wavelength of 700
nm
[See
next page
SBC3013 Cell
6
Biology
16. Which statement describes the functioning of photosystem II?
A. The electron vacancies in P680
B.
Light
are
filled
by
electrons derived from water.
chain in
energy excites electrons in the electron transport
a
photosynthetic
unit.
C. The
excitation
passed along to
is
molecule
a
of
P700
chlorophyll in the
photosynthetic unit.
D. The
P680
chlorophyll
donates
a
pair
of protons to
NADPH, which is thus
converted to NADP+.
are
17. Some photosynthetic organisms contain chloroplasts that lack photosystem II, yet
able to survive. The best way to detect the lack of photosystem II in these organisms
would be
-----
A. to test for CO2 fixation in the dark
B. to test for liberation of O2 in the
C. to do experiments to generate
D. to determine if
18. Carotenoids
properties in
are
they
often
They
reflect orange
B.
They
serve as
C.
They dissipate
D.
They
A.
an
action spectrum
thylakoids
found
in the
chloroplasts
in foods that
are
considered to
human nutrition. What related function do
A.
cover
19. What is the
have
light
Split water
have in
plants?
light.
accessory
pigments.
excessive
light
energy.
the sensitive chromosomes of the
primary
they
have antioxidant
function of the Calvin
plant.
cycle?
and release oxygen.
B. Use ATP to release carbon dioxide.
C. Transport RuBP out of the chloroplast.
D.
Synthesize simple
sugars from carbon dioxide.
[See
next page
7
,BC3013 Cell Biology
produce a 6 carbon
molecule, which is then split in two. After phosphorylation and reduction, what more
20. In the process of carbon
needs to
A.
happen
Regeneration
fixation, RuBP attaches
in the Calvin
a
CO2
to
cycle?
of rubisco.
B. Regeneration of ATP from ADP.
C. Inactivation of RuBP carboxylase enzyme.
D. Addition of
a
pair
of electrons from NADPH.
21. What is the term for metabolic
pathways that release stored energy by breaking
down complex molecules?
A. Anabolic pathways
B. Catabolic pathways
C. Fermentation pathways
D. Thermodynamic pathways
22. Where does
A.
glycolysis takes place?
Cytosol
B. Mitochondrial outer membrane
C. Mitochondrial inner membrane
D. Mitochondrial intermembrane space
23.
During cellular respiration
oxygen?
A. The citric acid
which process
or
event involved
directly in consuming
cycle.
B. The oxidation of pyruvate to acetyl CoA.
C. The phosphorylation of ADP to form ATP.
D. Accepting electrons at the end of the electron transport chain.
[See
next page
,)BC3013 Cell
24.
8
Biology
During glycolysis,
glucose is
when
catabolized to
glucose is
pyruvate, most of the energy of
-----
A. retained in the pyruvate
B. transferred
directly to ATP
C. stored in the NADH produced
D. transferred to ADP,
forming
ATP
25. In cellular respiration, when pyruvate enters mitochondrion via active transport,
is coenzyme A, a sulfur containing molecule derived from a B vitamin, added?
why
A. Because it drives the reaction that regenerates NAD+.
B. Because sulfur is needed for the molecule to enter the mitochondrion.
C. To provide
a
relatively
unstable molecule whose
acetyl portion
can
readily bind to
oxaloacetate.
D. In order to utilize this
product from
another
portion of
a
B vitamin which would otherwise be
a
waste
pathway.
26. Which location accumulates
acetyl
CoA
during
cellular
respiration?
A. Mitochondrial matrix
B. Mitochondrial outer membrane
C. Mitochondrial inner membrane
D. Mitochondrial intermembrane space
(C02)
27. Carbon dioxide
is released
during
which of the
following stages of
cellular
respiration?
A. Oxidative phosphorylation and fermentation.
B. The citric acid
C.
Glycolysis
cycle and oxidative phosphorylation.
and the oxidation of
D. Oxidation of
pyruvate to acetyl CoA.
pyruvate to acetyl CoA and the citric acid cycle.
[See
next page
SBC3013 Cell Biology
28. Which of the
A.
9
following
Converting
harvests the most chemical energy in cellular
respiration?
oxygen to ATP.
B. Chemiosmotic
phosphorylation.
C. Substrate-level phosphorylation.
D.
Transferring
electrons from
29. Which of the
following
transport chain, starting
A.
molecules to
pyruvate.
describes the sequence of electron carriers in the electron
with the least electronegative?
Fe-S, FMN, cytochromes (Cyt), ubiquinone
B. FMN, =e-s.
C.
organic
ubiquinone, cytochromes (Cyt)
Cytochromes (Cyt), FMN, ubiquinone, =e-s
D. Ubiquinone (Q), cytochromes (Cyt), FMN, FeeS
30. Which metabolic
A.
pathway is
common
to both cellular
respiration and
fermentation?
Glycolysis
B. The citric acid
cycle
C. Oxidative phosphorylation
D. The oxidation of pyruvate to
31.
acetyl
CoA
an organism.
Cytosine makes up 38% of the nucleotides in a sample of DNA from
will
be
thymine?
Approximately what percentage of the nucleotides in this sample
A. 12
B. 24
C.31
D.38
[See
next page
SBC3013 Cell
10
Biology
32. What kind of chemical bond is found between paired bases of the DNA double helix?
A. Ionic
B. Covalent
C.
Hydrogen
D.
Sulfhydryl
33. Which of this
reason
is the difference in DNA
replication
between
prokaryotes
and
eukaryotes?
A. The
prokaryotic
chromosome has
histones, whereas eukaryotic chromosomes do
not.
B.
Prokaryotic
chromosomes have
a
single origin of replication,
whereas
eukaryotic
chromosomes have many.
C. The rate of elongation during DNA replication is slower in prokaryotes than in
eukaryotes.
D.
Prokaryotes produce Okazaki fragments during DNA replication,
but
eukaryotes
do not.
34. What is meant
by the description "antiparallel" regarding the
strands that make up
DNA?
A. The
twisting
nature of DNA creates
B. One strand is
positively charged
nonparallel strands.
and the other is
negatively charged.
C. Base pairings create unequal spacing between the two DNA strands.
D. The 5' to 3' direction of
one
strand
runs
counter to the 5' to 3' direction of the other
strand.
35. Which enzyme
catalyzes the elongation of
a
DNA strand in the 5'
�
3' direction?
A. Primase
B. DNA
ligase
C. Topoisomerase
D. DNA
polymerase
III
[See
next page
SBC3013 Cell
Biology
11
36. Once transcribed,
includes
eukaryotic
mRNA
typically undergoes substantial
alteration that
-----
A. excision of introns
B. union with ribosomes
C.
linkage
to histone molecules
D. fusion mto circular forms known
as
plasm ids
37. Which kind of mutation resulted sickle-cell disease?
A. Point
B. Nonsense
C. Frameshift
D.
Nondisjunction
38. Which of the
following
A. Translation
B. Unlike
can
statement is true about
begin
in
prokaryotes?
while transcription is still in progress.
eukaryotes, prokaryotes require
C. Extensive RNA
protein synthesis
no
processing is required
initiation
before
or
elongation factors.
prokaryotic transcripts
can
be
translated.
D.
Prokaryotic
appropriate
39. The
complicated mechanisms
cellular organelles.
tryptophan
cells have
operon is
a
repressible
A. turned off
only when glucose
B. turned
only
on
when
glucose
on
to the
_
growth medium
present in the growth medium
C. turned off whenever tryptophan is added to the
D. turned
targeting proteins
operon that is
is present in the
is
for
only when tryptophan is present in
growth medium
the
growth
medium
[See
next page
SBC3013 Cell
Biology
40. When
no
longer binds
12
is taken up
to the
by the cell, it
operator.
binds to the repressor
so
that the repressor
A. operon
B. inducer
c. promoter
D. repressor
[See next page
SBC3013 Cell Biology
13
Section B (30
marks)
Instruction: Answer all questions
1.
Figure
1 shows the endomembrane
two distinct
are
organelles
system of a cell. Structure labelled as A and B is
system while structure labelled as C and D
of endomembrane
vesicles.
B
Goigi apparatus
Figure 1
a.
Describe
a
structural distinction between structure labelled
as
A and B.
[2 marks]
b.
Describe
a
functional distinction between the structures mentioned in question (a).
[2 marks]
[See
next page
SBC3013 Cell
c.
Biology
14
Imagine a protein that functions in structure labelled as 8 but requires modification in
Golgi apparatus before it can achieve that function. Describe the protein's path
through the cell, starting with the mRNA molecule that specifies the protein.
the
[4 marks]
d.
C and D contain products and are pinched off from Golgi
Describe one interacting mechanism distinction between these two
Vesicles labelled
apparatus.
as
vesicles.
[2 marks]
[See
next page
SBC3013 Cell
2.
Biology
15
Figure 2 shows the organization of thylakoid
Pq or plastoquinone, Pc or plastocyanin and
Photosystem
II
membrane and
Fd
or
ferredoxin
light
reaction process.
are
electron carriers.
Cytochrome
Photosystem
NADP+
complex
I
reductase
Light
To Calvin
Cycle
K
•
o
...
Figure 2
a.
When
light
photosystem, it will cause the conversion of compound F to
G. Identify compound F and molecule G.
strikes
become molecule
[2 marks]
b.
What will happen to H when
'Pq',
the mobile carrier,
moves
from photosystem II to
cytochrome complex?
[1 mark]
[See
next page
SBC3013 Cell
C.
Biology
16
When electron flow reaches the edge of thylakoid membrane, it will trigger the
conversion of substance I to substance J. Identify substance I and J.
[2 marks]
d.
Describe the process involved in converstorr of substance K to L.
[2 marks]
e.
thylakoid and enter the stroma to proceed with Calvin
What is the
cycle. Both substances are required in large amount during Calvin cycle.
main function of substance J and L in Calvin cycle?
Substance J and L will exit the
[1 mark]
f.
What will
happen
L could not break
light reaction if substance J could
its' phosphate atom in Calvin cycle.
to
not be oxidized and substance
[1 mark]
g.
During photosynthesis, plant
cycle. Assuming that
leaves, explain the process of
fixes carbon dioxide in Calvin
closed and oxygen build up inside the
fixative mechanism that will most likely to occur at such condition.
stomata
are
[1 mark]
[See
next page
SBC3013 Cell
3.
17
Biology
Figure
3 shows
an
overview of cellular respiration.
,-,-,----.
Electrons
Electrons carried
carried via
via NADH and
NADH
FADH
Metabolic stage 1
Metabolic stage 4
Glucose
c:::> Pyruvate
;
'--.,--,,-----.--.----'
�.-"."""
------.
Figure
a.
3
State
figure 3, which metabolic stage is involved in anaerobic respiration?
the location where this metabolic stage occurs in a cell?
Based
on
[2 marks]
b.
Metabolic stage 1 converted one molecule of glucose to two molecules of
and generated ATP. State another important yield from this metabolic stage.
pyruvate
[1 mark]
[See
next page
,//
SBC3013 Cell
c.
18
Biology
Which metabolic stage in
figure
3 produces the CO2 that you exhale?
[1 mark]
d.
Which metabolic stage in
figure
3 produces molecules that conserved the highest
potential energy? State the molecule.
[2 marks]
e.
of
figure 3 involves movement of electrons and releasing
this
chemiosmosis as occurs in
Briefly explain the process of
Which metabolic stage in
hydrogen
ions?
metabolic
stage?
[3 marks]
f.
Which metabolic
in the form of ATP?
stage in figure 3 produces the most energy
[1 mark]
[See
next page
SBC3013 Cell
Biology
19
Section C
Instruction: Answer any TWO
1.
(10 marks)
(2) questions
replication of lagging stands is synthesized discontinuously, as series of DNA
rragrner1l� known as Okazaki fragment. Describe the synthesis of a lagging strand.
The description should begin with reaction by primase and ends with DNA ligase.
DNA
[5 marks]
2.
DNA
serves
as
a
very important
core
template in both
DNA
replication and
transcription process.
a.
Compare
polymerase and RNA polymerase in terms of how they function,
of synthesis, requirement of primer, and the type of nucleotides
DNA
the direction
used.
[3 marks]
b.
the
During transcription process, suppose X-rays caused sequence change in
TATA box of a particular gene's promoter. How would it affect transcription of
the gene?
[2 marks]
3.
binding of RNA polymerase and
when lactose and glucose respectively are
scarcities on transcription of the lac operon?
Describe the
repressor to the inducible lac operon
What is the effect of these
scarce.
[5marksj
END OF QUESTIONS