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TRIDIA™ In Vitro Diagnostic Surfaces Improve sensitivity, reduce background TRIDIA™ In Vitro Diagnostic Surfaces The Success of Your Application Lies in its Surface The interface between non-biological solid supports and sensitive biological capture analytes is at the core of diagnostic assays. Advanced surface coatings from SurModics IVD optimize DNA, RNA, protein, and cell attachment for molecular diagnostic and immunoassay applications, reducing non-specific background and improving sensitivity. Versatile SurModics TRIDIA™ surface coatings bind molecules to a variety of surfaces and geometries and may be customized for selectivity using passivating polymers and reactive groups. For diagnostic devices including point of care assays, SurModics surface coatings improve flow characteristics through membranes and microfluidic channels. Other (non-analyte capture) surfaces from SurModics IVD increase the wettability, hemocompatibility or lubricity of surfaces in diagnostic instruments and other test components. Standard formats for evaluation purposes include glass slides, 96-well plates and POC devices/chips. TRIDIA Solutions for DNA/RNA Applications Bind amine-modified oligos, DNA or RNA. Common applications include DNA arrays, SNP analysis and array CGH. DNA Oligonucleotide Binding to Microarray Slide Surfaces 60,000 Signal (RFU) 50,000 TRIDIA NHS and TRIDIA HD surfaces have superior DNA binding compared to other common microarray surfaces. 40,000 30,000 20,000 Biotinylated oligonucleotide was attached either through a 5’amine group or hydroxyl group depending on the surface tested. Streptavidin-Cy5 was used for detection and quantification of oligo bound to surface. 10,000 0 TRIDIA NHS Description TRIDIA HD Epoxy Silane TRIDIA™ HD (High-Density) Surface TRIDIA™ NHS Surface Specially modified surface polymer for additional hydrophobicity for greater spotting densities per area of coated surface. Higher spotting densities than TRIDIA NHS surface, and the highest contact angle of SurModics’ surfaces. An amine-reactive polymeric top coating for covalent immobilization of DNA/RNA. Specialized surface polymer minimizes nonspecific binding of unwanted biomolecules in different sample types including blood and serum. Reactive Chemistry Product Code Amino Amino Aldehyde Amino Silane A Silane B Silane C • N-oxysuccinimide (NHS ester) DHD1 DN01 TRIDIA Surfaces—Three Distinct Features = = O N O O= N O O= O= N O O O O= N O = O = N = O O = Reactive Binding Group Binds antibodies, ligands, antigens, DNA, microRNA or other biomolecules. Increase signal by providing higher loading densities of the capture bioanalyte. Tailor for your needs—NHS esters, epoxide, biotin-avidin and others. O Passivating Polymer Prevents nonspecific binding of unwanted biomaterial in a 3D environment. Choose from passivating polymers to tailor polymer backbone and meet specific hydrophilic/hydrophobic needs. Photoreactive Group Uses PhotoLink® technology for fast, reliable covalent coupling of the polymer to a variety of materials including plastics, metals, glass and latex. TRIDIA Solutions for Protein Applications TRIDIA EP (Epoxy) Surfaces bind proteins (antibodies, antigens), PCR products or peptides. With TRIDIA BA (Biotin-Avidin) Surfaces, bind biotin-modified oligos and biotin-labeled DNA and cDNA, or biotinylated proteins (antibodies, antigens, peptides) specific to the bioanalyte. Choose the high-performance surface you need for applications including protein biosensors, cell capture and peptide arrays. Analysis of Troponin on a Protein Microarray ng/mL Troponin 120 Lower backgrounds and better sensitivity with TRIDIA Surfaces. The TRIDIA slide displays more distinct spots with consistently low background across the slide. 80 30 10 TRIDIA Competitor A Competitor B Epoxysilane Epoxysilane Troponin I sandwich immunoassay. Capture antibody–mouse anti-troponin I (AbCam); primary detection antibody-rabbit anti-troponin I antibody; secondary detection antibody-goat anti-rabbit-Cy3. Scanned on Axon 4200 AL scanner in the 632 nm channel. TRIDIA™ EP (Epoxy) Surface TRIDIA™ BA (Biotin-Avidin) Surface Description Contains an epoxide reactive group for binding. Proteins bind via their exposed amine, thiol, and hydroxyl groups. Polymeric topcoat covalently attached to an avidin derivative via an NHS ester. Helps minimize nonspecific binding of unwanted biomolecules. Avidin derivative allows easy attachment of biotinylated antibodies for capture and analysis of biomarkers of interest. Reactive Chemistry Epoxide Biotin-avidin derivative Product Code DEPX Custom only TRIDIA™ In Vitro Diagnostic Surfaces Apply TRIDIA diagnostic surfaces to multiple surface types including membranes, microtiter plates, beads, and more. Using our custom coating services, apply SurModics’ proprietary polymer coating to your diagnostic device with your choice of reactive chemistry. With SurModics IVD surfaces, attach your biomolecule and eliminate background with our passivating polymer. Custom Surfaces • Combine your choice of passivating polymers with various reactive chemistries (NHS-ester, epoxide, biotin-avidin, hydroxyl, or aldehyde) • Utilize SurModics’ PhotoLink process to covalently link molecules to various surfaces • Improve flow characteristics for membranes and microfluidic channels with SurModics wettable surfaces • Reduce the adherence of interfering blood components with SurModics hemocompatible surfaces Ordering Information Product Code Product Available Sizes Shelf Life* DN01 CodeLink® Activated Microarray Slides (TRIDIA NHS Surface) 25 slides per box 10 months at R.T. DHD1 CodeLink® HD Activated Microarray Slides (TRIDIA HighDensity Surface) 23 slides per box 12 months at R.T. DEPX TRIDIA™ EP (Epoxy) Microarray Slides 25 slides per box 24 months at R.T. SC02 StabilCoat® Plus Microarray Stabilizer 50 mL and 1000 mL 36 months at 4°C SG02 StabilGuard® Choice Microarray Stabilizer (BSA-Free) 50 mL and 1000 mL 36 months at 4°C * Although the recommended storage temperature is 4°C for all of the protein stabilizers, the products are shipped at ambient temperature. Extensive stability studies have shown that storage at ambient temperature does not affect the quality or efficacy of these products. To place an order or request a sample: Toll-free: 1-800-755-7793 (North America) Phone: 952-500-7200 Fax: 952-500-7201 Email: [email protected] For additional information, visit www.surmodics.com Technical assistance: [email protected] PhotoLink and TRIDIA are registered trademarks of SurModics, Inc. and/or its affiliates. CodeLink is a trademark of a GE Healthcare Company. SurModics IVD, Inc. 9924 West 74th Street Eden Prairie, MN 55344 USA Toll-free: 1-800-755-7793 Phone: 952-500-7200 Fax: 952-500-7201 www.surmodics.com shop.surmodics.com © 2015 SurModics IVD, Inc. All rights reserved. TRID-0215-04