Download TRIDIA™ In Vitro Diagnostic Surfaces

TRIDIA™ In Vitro Diagnostic Surfaces
Improve sensitivity, reduce background
TRIDIA™ In Vitro Diagnostic Surfaces
The Success of Your Application Lies in its Surface
The interface between non-biological solid supports and sensitive biological capture analytes is at the core
of diagnostic assays. Advanced surface coatings from SurModics IVD optimize DNA, RNA, protein, and cell
attachment for molecular diagnostic and immunoassay applications, reducing non-specific background and
improving sensitivity.
Versatile SurModics TRIDIA™ surface coatings bind molecules to a variety of surfaces and geometries and may
be customized for selectivity using passivating polymers and reactive groups.
For diagnostic devices including point of care assays, SurModics surface coatings improve flow characteristics
through membranes and microfluidic channels. Other (non-analyte capture) surfaces from SurModics IVD
increase the wettability, hemocompatibility or lubricity of surfaces in diagnostic instruments and other test
components.
Standard formats for evaluation purposes include glass slides, 96-well plates and POC devices/chips.
TRIDIA Solutions for DNA/RNA Applications
Bind amine-modified oligos, DNA or RNA. Common applications include DNA arrays, SNP analysis and array CGH.
DNA Oligonucleotide Binding to Microarray Slide Surfaces
60,000
Signal (RFU)
50,000
TRIDIA NHS and TRIDIA HD surfaces have
superior DNA binding compared to other
common microarray surfaces.
40,000
30,000
20,000
Biotinylated oligonucleotide was attached either through a
5’amine group or hydroxyl group depending on the surface tested.
Streptavidin-Cy5 was used for detection and quantification of oligo
bound to surface.
10,000
0
TRIDIA
NHS
Description
TRIDIA
HD
Epoxy
Silane
TRIDIA™ HD (High-Density) Surface
TRIDIA™ NHS Surface
Specially modified surface polymer for additional
hydrophobicity for greater spotting densities per
area of coated surface. Higher spotting densities than
TRIDIA NHS surface, and the highest contact angle of
SurModics’ surfaces.
An amine-reactive polymeric top coating for
covalent immobilization of DNA/RNA. Specialized
surface polymer minimizes nonspecific binding of
unwanted biomolecules in different sample types
including blood and serum.
Reactive
Chemistry
Product Code
Amino Amino Aldehyde Amino
Silane A Silane B
Silane C
• N-oxysuccinimide (NHS ester)
DHD1
DN01
TRIDIA Surfaces—Three Distinct Features
=
=
O
N
O
O=
N
O
O=
O=
N
O
O
O
O=
N
O
=
O
=
N
=
O
O
=
Reactive Binding Group
Binds antibodies, ligands, antigens, DNA, microRNA
or other biomolecules. Increase signal by providing
higher loading densities of the capture bioanalyte.
Tailor for your needs—NHS esters, epoxide,
biotin-avidin and others.
O
Passivating Polymer
Prevents nonspecific binding of unwanted
biomaterial in a 3D environment. Choose from
passivating polymers to tailor polymer backbone
and meet specific hydrophilic/hydrophobic needs.
Photoreactive Group
Uses PhotoLink® technology for fast, reliable
covalent coupling of the polymer to a variety of
materials including plastics, metals, glass and latex.
TRIDIA Solutions for Protein Applications
TRIDIA EP (Epoxy) Surfaces bind proteins (antibodies, antigens), PCR products or peptides. With TRIDIA BA (Biotin-Avidin)
Surfaces, bind biotin-modified oligos and biotin-labeled DNA and cDNA, or biotinylated proteins (antibodies, antigens,
peptides) specific to the bioanalyte. Choose the high-performance surface you need for applications including protein
biosensors, cell capture and peptide arrays.
Analysis of Troponin
on a Protein Microarray
ng/mL Troponin
120
Lower backgrounds and better sensitivity with TRIDIA Surfaces.
The TRIDIA slide displays more distinct spots with consistently
low background across the slide.
80
30
10
TRIDIA
Competitor A Competitor B
Epoxysilane Epoxysilane
Troponin I sandwich immunoassay. Capture antibody–mouse anti-troponin I (AbCam);
primary detection antibody-rabbit anti-troponin I antibody; secondary detection
antibody-goat anti-rabbit-Cy3. Scanned on Axon 4200 AL scanner in the 632 nm channel.
TRIDIA™ EP (Epoxy) Surface
TRIDIA™ BA (Biotin-Avidin) Surface
Description
Contains an epoxide reactive group for binding.
Proteins bind via their exposed amine, thiol, and
hydroxyl groups.
Polymeric topcoat covalently attached to an avidin
derivative via an NHS ester. Helps minimize nonspecific binding of unwanted biomolecules. Avidin
derivative allows easy attachment of biotinylated
antibodies for capture and analysis of biomarkers
of interest.
Reactive
Chemistry
Epoxide
Biotin-avidin derivative
Product Code
DEPX
Custom only
TRIDIA™ In Vitro Diagnostic Surfaces
Apply TRIDIA diagnostic surfaces to multiple surface types including membranes, microtiter
plates, beads, and more. Using our custom coating services, apply SurModics’ proprietary
polymer coating to your diagnostic device with your choice of reactive chemistry. With
SurModics IVD surfaces, attach your biomolecule and eliminate background with our
passivating polymer.
Custom Surfaces
• Combine your choice of passivating polymers with various reactive chemistries
(NHS-ester, epoxide, biotin-avidin, hydroxyl, or aldehyde)
• Utilize SurModics’ PhotoLink process to covalently link molecules to various surfaces
• Improve flow characteristics for membranes and microfluidic channels with SurModics
wettable surfaces
• Reduce the adherence of interfering blood components with SurModics
hemocompatible surfaces
Ordering Information
Product
Code
Product
Available Sizes
Shelf Life*
DN01
CodeLink® Activated Microarray Slides (TRIDIA NHS Surface)
25 slides per box
10 months at R.T.
DHD1
CodeLink® HD Activated Microarray Slides (TRIDIA HighDensity Surface)
23 slides per box
12 months at R.T.
DEPX
TRIDIA™ EP (Epoxy) Microarray Slides
25 slides per box
24 months at R.T.
SC02
StabilCoat® Plus Microarray Stabilizer
50 mL and 1000 mL
36 months at 4°C
SG02
StabilGuard® Choice Microarray Stabilizer (BSA-Free)
50 mL and 1000 mL
36 months at 4°C
* Although the recommended storage temperature is 4°C for all of the protein stabilizers, the products are shipped at ambient temperature.
Extensive stability studies have shown that storage at ambient temperature does not affect the quality or efficacy of these products.
To place an order or request a sample:
Toll-free: 1-800-755-7793 (North America)
Phone: 952-500-7200
Fax: 952-500-7201
Email: [email protected]
For additional information, visit www.surmodics.com
Technical assistance: [email protected]
PhotoLink and TRIDIA are registered trademarks of SurModics, Inc. and/or its affiliates.
CodeLink is a trademark of a GE Healthcare Company.
SurModics IVD, Inc.
9924 West 74th Street
Eden Prairie, MN 55344 USA
Toll-free: 1-800-755-7793
Phone: 952-500-7200
Fax: 952-500-7201
www.surmodics.com
shop.surmodics.com
© 2015 SurModics IVD, Inc.
All rights reserved. TRID-0215-04