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Version: 1 Revision Date: 01/30/2014 Purified anti-mouse IL-10 Antibody Catalog# / Size 521801 / 50 µg 521802 / 500 µg Clone MK10A6 (See other available formats) Other Names Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF), T cell growth inhibitory factor (TGIF) Isotype Rat IgG1, κ Ave. Rating ★★★★★ 0 reviews Description IL-10 was first described as a cytokine that is produced by T helper 2 (Th2) cell clones. It inhibits interferon gamma (IFN-γ) synthesis in Th1 cell, and therefore it was initially called cytokine synthesis inhibiting factor (CSIF). Macrophages are the main source of IL-10, and its secretion can be stimulated by endotoxin (via Toll-like receptor 4, NF-κB dependent), tumor necrosis factor TNF-α (via TNF receptor p55, NF-κB-dependent), catecholamines, and IL-1. IL-10 controls inflammatory processes by suppressing the expression of proinflammatory cytokines, chemokines, adhesion molecules as well as antigen-presenting and costimulatory molecules in monocytes/macrophages, neutrophils, and T cells. IL-10 inhibits the production of proinflammatory mediators by monocytes and macrophages such as endotoxin- and IFNγ-induced release of IL-1α, IL-6, IL8, G-CSF, GM-CSF, and TNF-α. In addition, it enhances the production of anti-inflammatory mediators such as IL-1RA and soluble TNF-α receptors. IL10 inhibits the capacity of monocytes and macrophages to present antigen to T cells. This is realized by downregulation of constitutive and IFNγinduced cell surface levels of MHC class II, of costimulatory molecules such as CD86, and of some adhesion molecules such as CD58. Reactivity Mouse Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation The antibody was purified by affinity chromatography. Concentration 0.5 mg/ml Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C. Application ELISA Capture - Quality tested Recommended Usage Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-8 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 2000 to 31.3 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application. Application Notes ELISA Capture: The purified MK10A6 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated JES5-16E3 antibody (Cat. No. 505003) as the detecting antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard. Note: For testing mouse IL-10 in serum, plasma or supernatant, BioLegend's LEGEND MAX™ Kits (Cat. No. 431417 & 431418) are specially developed and recommended. RRID AB_2561713 (BioLegend Cat. No. 521801) AB_2561714 (BioLegend Cat. No. 521802) Antigen Details Structure Acid-labile cytokine, dimer, 17-21 kD (Mammalian) Distribution IL-10 is produce by Th2 cells, macrophages, DCs, B cells, CD8+ T cells, regulatory T cells (Tregs), Th1 cells and Th17 cells. In addition, IL-10 is expressed by monocytes, B cells, eosinophils, and mast cells. Function IL-10 is an immunoregulatory cytokine. Its main function is the limitation and termination of inflammatory responses and the regulation of differentiation and proliferation of several immune cells such as T cells, B cells, natural killer cells, antigen-presenting cells, mast cells, and granulocytes. Interaction IL-10R is expressed in monocytes, NK, B, and T cells. In addition Langerhans cells, dermal dendritic cells, eosinophils, mast cells, and endothelial cells can respond to IL-10. Ligand Receptor IL-10 binds to their receptors IL-10R1 and IL-10R2, and initiates a STAT3-dependent signaling cascade. Antigen References 1. Fiorentino DF, et al. 1989. J. Exp. Med. 170:2081. 2. Ho AS, et al. 1993. Proc. Natl. Acad. Sci. USA 90:11267. 3. Hart PH, et al. 1996. J. Immunol. 157:3672. 4. Asadullah K, et al. 2003. Pharmacol. Rev. 55:241. 5. Mosser DM and Zhang X. 2008. Immunol. Rev. 226:205. 6. Maynard CL and Weaver CT. 2008. Immunol. Rev. 226:219. Gene ID 16153 UniProt View information about IL-10 on UniProt.org Documentation Technical Data Sheet (pdf) Certificate of Analysis Safety Data Sheet Related Protocols Sandwich ELISA Protocol Related FAQs There are no FAQs for this product. Related Categories Cytokines & Chemokines > Mouse Cytokine & Chemokine Research > Mouse ELISA Antibody Pairs > IL-10 > Cytokines & Chemokines > Mouse Cytokine & Chemokine Research > Antibodies to Cytokines/Chemokines > IL-10 > For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. *These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use. BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587