Download Purified anti-mouse IL-10 Antibody

Version: 1
Revision Date: 01/30/2014
Purified anti-mouse IL-10 Antibody
Catalog# / Size
521801 / 50 µg
521802 / 500 µg
Clone
MK10A6 (See other available formats)
Other Names
Interleukin-10, Cytokine synthesis inhibitory factor (CSIF),
B cell derived T cell growth factor (B-TCGF), T cell
growth inhibitory factor (TGIF)
Isotype
Rat IgG1, κ
Ave. Rating
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Description
IL-10 was first described as a cytokine that is produced by T helper 2 (Th2)
cell clones. It inhibits interferon gamma (IFN-γ) synthesis in Th1 cell, and
therefore it was initially called cytokine synthesis inhibiting factor (CSIF).
Macrophages are the main source of IL-10, and its secretion can be
stimulated by endotoxin (via Toll-like receptor 4, NF-κB dependent), tumor
necrosis factor TNF-α (via TNF receptor p55, NF-κB-dependent),
catecholamines, and IL-1. IL-10 controls inflammatory processes by
suppressing the expression of proinflammatory cytokines, chemokines,
adhesion molecules as well as antigen-presenting and costimulatory
molecules in monocytes/macrophages, neutrophils, and T cells. IL-10
inhibits the production of proinflammatory mediators by monocytes and
macrophages such as endotoxin- and IFNγ-induced release of IL-1α, IL-6, IL8, G-CSF, GM-CSF, and TNF-α. In addition, it enhances the production of
anti-inflammatory mediators such as IL-1RA and soluble TNF-α receptors. IL10 inhibits the capacity of monocytes and macrophages to present antigen
to T cells. This is realized by downregulation of constitutive and IFNγinduced cell surface levels of MHC class II, of costimulatory molecules such
as CD86, and of some adhesion molecules such as CD58.
Reactivity
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application
ELISA Capture - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture
applications, a concentration range of 2-8 µg/ml is recommended. To obtain a linear standard
curve, serial dilutions of IL-10 recombinant protein ranging from 2000 to 31.3 pg/ml are
recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal
performance for each application.
Application Notes
ELISA Capture: The purified MK10A6 antibody is useful as the capture antibody in a sandwich
ELISA assay, when used in conjunction with the biotinylated JES5-16E3 antibody (Cat. No.
505003) as the detecting antibody and recombinant mouse IL-10 (Cat. No. 575809) as the
standard.
Note: For testing mouse IL-10 in serum, plasma or supernatant, BioLegend's LEGEND MAX™
Kits (Cat. No. 431417 & 431418) are specially developed and recommended.
RRID
AB_2561713 (BioLegend Cat. No. 521801)
AB_2561714 (BioLegend Cat. No. 521802)
Antigen Details
Structure
Acid-labile cytokine, dimer, 17-21 kD (Mammalian)
Distribution
IL-10 is produce by Th2 cells, macrophages, DCs, B cells, CD8+ T cells, regulatory T cells
(Tregs), Th1 cells and Th17 cells. In addition, IL-10 is expressed by monocytes, B cells,
eosinophils, and mast cells.
Function
IL-10 is an immunoregulatory cytokine. Its main function is the limitation and termination of
inflammatory responses and the regulation of differentiation and proliferation of several
immune cells such as T cells, B cells, natural killer cells, antigen-presenting cells, mast cells,
and granulocytes.
Interaction
IL-10R is expressed in monocytes, NK, B, and T cells. In addition Langerhans cells, dermal
dendritic cells, eosinophils, mast cells, and endothelial cells can respond to IL-10.
Ligand Receptor
IL-10 binds to their receptors IL-10R1 and IL-10R2, and initiates a STAT3-dependent signaling
cascade.
Antigen References
1. Fiorentino DF, et al. 1989. J. Exp. Med. 170:2081.
2. Ho AS, et al. 1993. Proc. Natl. Acad. Sci. USA 90:11267.
3. Hart PH, et al. 1996. J. Immunol. 157:3672.
4. Asadullah K, et al. 2003. Pharmacol. Rev. 55:241.
5. Mosser DM and Zhang X. 2008. Immunol. Rev. 226:205.
6. Maynard CL and Weaver CT. 2008. Immunol. Rev. 226:219.
Gene ID
16153
UniProt
View information about IL-10 on UniProt.org
Documentation
Technical Data Sheet (pdf)
Certificate of Analysis
Safety Data Sheet
Related Protocols
Sandwich ELISA Protocol
Related FAQs
There are no FAQs for this product.
Related Categories
Cytokines & Chemokines > Mouse Cytokine & Chemokine Research > Mouse ELISA Antibody Pairs > IL-10 >
Cytokines & Chemokines > Mouse Cytokine & Chemokine Research > Antibodies to Cytokines/Chemokines > IL-10 >
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