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Transcript
Investigate protein assemblies with molecular weight up to 1 MDa! 40 KDa MBP 80 KDa MSG 500 KDa TET-2 1 MDa chapronin Structural studies… Dynamics, Interactions, Mechanisms … Introduce NMR-Bio SLAM kits in a perdeuterat ed minimum medium, to achieve high incorporation levels of 13 CH3 isotopomer only in a single type of methyl groups (i.e. ValproS, Ala−β, Thr−γ etc. …) without detectable scrambling. 2D Methyl-TROSY spectra of a 468 kDa protein assembly. Courtesy of Dr. Boisbouvier Alanine- β Valine-proS Reduce spectral overlaps using NMR-Bio combinatori al labeling kits, designed to label simultaneously different types of Methyl probes well separated in Methyl-TROSY spectra. Recommended kits: SLAM-Aβ / I δ1 / M ε / Tγ / VproS NMR-Bio SLAM-Aβ Detection of long-range nOes in supramolecular assemblies: NMR-Bio scrambling free labeling solutions are optimized for the extraction of precise and long-range nOe distance restraints between methyl probes in perdeut erated proteins. Compared to standard 2-keto acids, NMR-Bio acetolact ate precursors increas e sensitivity by up to a factor of 4, allowing for the detection of structurally meaningful long-range and intermolecular nOes restraints. Filtering of inter-molecular nOes in supramolecular assemblies: Use NMR-Bio kits to filter inter- from intramolecular nOes and get more precise structural information to refine your homo-oligomeric prot ein structure. Using our regio- and st ereo-specific labeling (ie. Ile-γ2, Il e-δ1, LeuproS, Leu-proR, Val-proS and Val-proR), the intermolecular nOes filtering of the same residue is achievable! Recommended kits: DLAM- AβIδ1 / AβMε / Iδ1Mε / Iδ1Tγ / Iδ1VproS / Iγ2VproR / Iδ1LproS / Iγ2LproR / Mε VproS / Mε Tγ ; TLAM- AβIδ1VproS / AβIδ1Mε / Iδ1Mε VproS NMR-Bio SLAM-Vp ro S References: Kerfah et al., Curr Opin Struct Biol. 2015, 32:113-22; Gans et al., Angew Chem Int Ed Engl. 2010; 8;49(11):1958-62 Example of 2D extract of 3D CCH NOESY experiment acquired on TET protein (460kDa) produced in U-[ 2 H] M9 medium supp lemented with DLAM-Iδ1 VproS. T he detected nOes are plotted in the PDB struture. Courtesy of Dr. Boisbouvier NMR-Bio kits Our patented precursors are specifically deuterated and supplied as frozen user-friendly kits ready to use without requirement of any further chemical modification. All NMR-Bio kits are calibrated for addition into of deuterated M9 culture medium prior to induction. NMR-Bio kits have been optimized to incorporate 13CH3 or 13CHD2 isotopomers in selected methyl groups of proteins, with the possibility to incorporate a linear 13C spin system connecting the specifically labeled methyl groups to the backbone nuclei. Kits are provided with precise protocols extensively tested in-vivo to ensure optimal incorporation of isotopes in targeted methyl groups without detectable scrambling in other positions. NMR-Bio SLAM kits LVproS 13CH3 LVproR 13CH3 Aβ Iγ2 Iδ1 Mε Tγ VproS VproR L proS L proR 13CH3 13CH3 13CH3 13CH3 13CH3 13CH3 13CH3 13CH3 13CH3 240 € 390 € 450€ 270 € 60 € 330 € 300 € 300 € 470 € 330 € 330 € Prices* (1 kit for 1 L of culture) Examples of kits 13 CH3 groups Labeled Prices* (1 kit for 1 L. of culture ) DLAM- I δ1 Mε Ile δ1 Metε 380 € DLAM-Aβ I δ1 Ala β Ile δ1 490 € DLAM- I δ1 VproS Ile δ1 ValproS 350 € DLAM- I δ1 LproS Ile δ1 LeuproS 380 € DLAM- I γ2 VproR Ile γ2 ValproR 650 € DLAM- I γ2 LproR Ile γ2 LeuproR 510 € DLAM-Aβ Mε Ala β Metε 770 € DLAM- MεVproS Metε ValproS 620 € DLAM- MεLproS Metε LeuproS 650 € DLAM-I δ1 Tγ Ile δ1 Thr γ 390 € DLAM- MεTγ Metε Thr γ 620 € TLAM-I δ1 MεVproS Ile δ1 Metε ValproS 670 € TLAM-I δ1 MεLproS Ile δ1 Metε LeuproS 690 € TLAM-Aβ I δ1 VproS Ala β Ile δ1 ValproS 790€ TLAM-Aβ I δ1 LproS Ala β Ile δ1 LeuproS 830 € TLAM-Aβ I δ1 Mε Ala β Ile δ1 Metε 890 € Visit the NMR-Bio booth and get 10% discount on quotes issued during the conference ! – ACADEMIC RESEARCH USEONLY – PRINTED INFRANCE – 03-2017 * The listed prices exclude shipping fees and importation tax. Discounts apply for larger quantities. For specific quote contact us at [email protected].