Download NMR-BIO kits for large proteins labeling

Investigate protein assemblies with
molecular weight up to 1 MDa!
40 KDa
MBP
80 KDa
MSG
500 KDa
TET-2
1 MDa
chapronin
Structural studies…
Dynamics, Interactions, Mechanisms …
Introduce NMR-Bio SLAM kits in a perdeuterat ed minimum
medium, to achieve high incorporation levels of 13 CH3
isotopomer only in a single type of methyl groups (i.e. ValproS, Ala−β, Thr−γ etc. …) without detectable scrambling.
2D Methyl-TROSY spectra of a
468
kDa
protein assembly.
Courtesy of Dr. Boisbouvier
Alanine- β
Valine-proS
Reduce spectral overlaps using NMR-Bio combinatori al
labeling kits, designed to label simultaneously different types
of Methyl probes well separated in Methyl-TROSY spectra.
Recommended kits: SLAM-Aβ / I δ1 / M ε / Tγ / VproS
NMR-Bio SLAM-Aβ
Detection of long-range nOes in supramolecular
assemblies: NMR-Bio scrambling free labeling solutions
are optimized for the extraction of precise and long-range
nOe distance restraints between methyl probes in
perdeut erated proteins. Compared to standard 2-keto acids,
NMR-Bio acetolact ate precursors increas e sensitivity by up
to a factor of 4, allowing for the detection of structurally
meaningful long-range and intermolecular nOes restraints.
Filtering of inter-molecular nOes
in supramolecular
assemblies: Use NMR-Bio kits to filter inter- from intramolecular nOes and get more precise structural information
to refine your homo-oligomeric prot ein structure. Using our
regio- and st ereo-specific labeling (ie. Ile-γ2, Il e-δ1, LeuproS, Leu-proR, Val-proS and Val-proR),
the intermolecular nOes filtering of the same residue is achievable!
Recommended kits: DLAM- AβIδ1 / AβMε / Iδ1Mε / Iδ1Tγ / Iδ1VproS /
Iγ2VproR / Iδ1LproS / Iγ2LproR / Mε VproS / Mε Tγ ; TLAM- AβIδ1VproS /
AβIδ1Mε / Iδ1Mε VproS
NMR-Bio SLAM-Vp ro S
References:
Kerfah et al., Curr Opin Struct Biol. 2015,
32:113-22; Gans et al., Angew Chem Int Ed
Engl. 2010; 8;49(11):1958-62
Example of 2D extract of 3D
CCH
NOESY
experiment
acquired on TET protein
(460kDa) produced in U-[ 2 H]
M9 medium supp lemented with
DLAM-Iδ1 VproS. T he detected
nOes are plotted in the PDB
struture. Courtesy of Dr.
Boisbouvier
NMR-Bio kits
Our patented precursors are
specifically deuterated and supplied as frozen user-friendly kits
ready to use without requirement of any further chemical
modification. All NMR-Bio kits are calibrated for addition into
of deuterated M9 culture medium prior to induction. NMR-Bio
kits have been optimized to incorporate 13CH3 or 13CHD2
isotopomers in selected methyl groups of proteins, with the
possibility to incorporate a linear 13C spin system connecting the
specifically labeled methyl groups to the backbone nuclei. Kits
are provided with precise protocols extensively tested in-vivo to
ensure optimal incorporation of isotopes in targeted methyl
groups without detectable scrambling in other positions.
NMR-Bio
SLAM kits
LVproS
13CH3
LVproR
13CH3
Aβ
Iγ2
Iδ1
Mε
Tγ
VproS
VproR
L proS
L proR
13CH3
13CH3
13CH3
13CH3
13CH3
13CH3
13CH3
13CH3
13CH3
240 €
390 €
450€
270 €
60 €
330 €
300 €
300 €
470 €
330 €
330 €
Prices*
(1
kit for 1 L
of culture)
Examples of kits
13
CH3 groups Labeled
Prices* (1 kit for 1 L. of culture )
DLAM- I δ1 Mε
Ile δ1 Metε
380 €
DLAM-Aβ I δ1
Ala β Ile δ1
490 €
DLAM- I δ1 VproS
Ile δ1 ValproS
350 €
DLAM- I δ1 LproS
Ile δ1 LeuproS
380 €
DLAM- I γ2 VproR
Ile γ2 ValproR
650 €
DLAM- I γ2 LproR
Ile γ2 LeuproR
510 €
DLAM-Aβ Mε
Ala β Metε
770 €
DLAM- MεVproS
Metε ValproS
620 €
DLAM- MεLproS
Metε LeuproS
650 €
DLAM-I δ1 Tγ
Ile δ1 Thr γ
390 €
DLAM- MεTγ
Metε Thr γ
620 €
TLAM-I δ1 MεVproS
Ile δ1 Metε ValproS
670 €
TLAM-I δ1 MεLproS
Ile δ1 Metε LeuproS
690 €
TLAM-Aβ I δ1 VproS
Ala β Ile δ1 ValproS
790€
TLAM-Aβ I δ1 LproS
Ala β Ile δ1 LeuproS
830 €
TLAM-Aβ I δ1 Mε
Ala β Ile δ1 Metε
890 €
Visit the NMR-Bio booth and get
10% discount on quotes issued
during the conference !
– ACADEMIC RESEARCH USEONLY –
PRINTED INFRANCE – 03-2017
* The listed prices exclude shipping fees and
importation tax. Discounts apply for larger
quantities. For specific quote contact us at
[email protected].