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Stage proposé par Geraldine DELBES
« Associée de recherche au Centre de Recherche en Sante de McGill »
Nom et adresse du Laboratoire :
Urology research labs, McGill University
Royal Victoria Hospital, H6-48
687 Pine Avenue west
Montreal, Quebec, H3A 1A1
Canada
Téléphone : 001-514-934-1934 est 34574
Mail : [email protected] and [email protected]
Directeur du Laboratoire : Peter Chan
Titre : Translational regulation during spermiogenesis.
Projet de stage : situation du sujet, objectif du stage, approches expérimentales
During spermiogenesis, several proteins that are needed for the proper male germ cell
differentiation are under translational control. Such proteins are expressed at very specific
steps in elongating spermatids. We have recently shown that Poly(A) Binding Protein (PABP)
interacting protein 2 (Paip2) is a major translational regulator involved in the maturation of
male germ cells and male fertility in mice. Using proteomic profiling, we identified that 29
proteins were under translational control of Paip2 regulation in elongating spermatids from
wild type (WT) mice and transgenic mice in which both Paip2A and Pai2B genes were
abrogated.
The goal of this research project is to evaluate is such controls exists in human. Using
human testicular biopsies, expression and cellular localization of the Paip2 machinery
(PAIP2A, PAIP2B, PABP) will be evaluated using IHC and western blot.
Techniques mises en œuvre par le stagiaire :
Histology, paraffin section, imunohistochemistry, image analysis, PCR, western blot
Publications du responsable de stage au cours des 5 dernières années :
- S. Larcher; G. Delbès, B. Robaire and V. Yargeau Degradation of 17α-ethinylestradiol by ozonation - identification of the byproducts and assessment of their estrogenicity and toxicity. Environment International. 2011. Sous presse
- A. Yanagiya, G. Delbès, B. Robaire, N. Sonenberg. Translational Control in Late Spermiogenesis by the PolyA Binding Protein
Partner, Paip2A. J Clin Invest. 2010 Sep 1;120(9):3389-400
- G. Delbès, F. Vaisheva, T. Luu, L. Marcon, B. F. Hales, B. Robaire. Reversibility of the Effects of the Chemotherapeutic
Regimen for Non-Hodgkin Lymphoma, Cyclophosphamide, Doxorubicin, Vincristine, and Prednisone, on the Male Rat
Reproductive System and Progeny Outcome. Reprod Toxicol. 2010 Jun;29(3):332-8.
- G. Delbès, D. Chan, P. Pakarinen, B.F. Hales, J.M. Trasler, B. Robaire. Impact of the Chemotherapy Cocktail Used to Treat
Testicular Cancer on the Gene Expression Profile of Male Germ Cells. Biol Reprod. 2009 Feb;80(2):320-7.
- G. Delbès, C. Duquenne, J. Szencker, J. Taccoen, R. Habert, C. Levacher. Developmental changes in testicular sensitivity to
estrogens throughout fetal and neonatal life. Toxicol Sci. 2007 Sep;99(1):234-43.
- F. Vaisheva, G. Delbès, B.F. Hales, B. Robaire. Effects of combinaition chemotherapy with cyclophosphamide, doxorubicin,
vincristine and prednisone (CHOP) on the male rat reproductive system and progeny outcome. J Androl. 2007 JulAug;28(4):578-87.
- G. Delbès, B.F. Hales, B. Robaire. Effect of the chemotherapy cocktail used to treat testicular cancer on sperm chromatin
integrity. J Androl. 2007 Mar-Apr;28(2):241-9; discussion 250-1.
- G. Livera, G. Delbès, C. Pairault, V. Rouiller Fabre, R. Habert. Organotypic culture, a powerful model for studying rat and
mouse fetal testis development. Cell Tissue Res, 2006 324(3):507-21.
CHAPITRE ET REVUES:
- R.J. Sherins, G. Delbès. Is there a Decline in Sperm Counts in Men? In Handbook of Andrology, 2nd Edn (ed B. Robaire and P.
Chan) 2010.
- G. Delbès, B.F. Hales, B. Robaire. Toxicants and human sperm chromatin integrity. Mol Hum Reprod. 2010 Jan;16(1):14-22.
- G. Delbès, C. Levacher, R. Habert. Estrogen effects on fetal and neonatal testicular development. Reproduction. 2006
Oct;132(4):527-38.
- R. Habert, G. Delbès, C. Duquenne, G. Livera, C. Levacher. Effects of estrogens on the development of the testis during fetal and
neonatal life. Gynecol Obstet Fertil. 2006, Oct;34(10):970-97.