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2014 MRC성과발표회
Abstract Information
[
] 심포지엄 구연 발표
[ O ] 기초의과학 학문후속세대 발표
Category (연제와 관련있는 분야에 모두 표시해 주십시오)
[
] Aging and age-related diseases
[
] Alternative medicine
[
] Anatomical methodology
[
] Anticancer strategies
[
] Bioengineering and technology
[
] Biology of development
[
] Biomarkers for personalized medicine
[
] Biomedical informatics
[
] Cancer biology (oncological sciences)
[
] Cellular injuries, death, apoptosis, autophagy
[
] Clinical pharmacology
[ O ] Craniofacial diseases
[ O ] Developmental Biology and Cell Differentiation
[
] DNA Replication, DNA Damage and Cell Cycle
[
] Drug: mechanism of action & toxicity
[
] Endocrinology and metabolism
[
] Enzyme: mechanism of action and regulation
[ O ] Gene expression
[
] Gene-, Cell-, & Immunotherapy
[
] Genetics and Epigenetics
[
] Gross anatomy
[
] Herb medicine and phytochemicals
[ O ] Histology of organs
[
] Immunology
[
] Inflammation and tissue injury
[
] Infectious diseases: bacteriology, virology and mycology
[
] Ion, ion channel & excitability
[
] Membrane transport & secretion
[
] Metabolic diseases
[
] Molecular and Cellular Aspects of Behaviour
[
] Neuroscience and Neurobiology
[
] Novel Targets for Drug Development and Screening Candidates
[
] Omics and Systems Biology
[
] Orofacial Diseases
[
] Protein Biochemistry: Structure, Function, and Modification
[
] Parasitology and parasitic diseases
[
] Pharmacogenetics
[
] Preventive medicine
[
] RNA Biology and Application
[
] Signal transduction
[
] Stem cells and regeneration
[
] Therapeutic approaches of Korean Medicine
[
] Vascular Biology and Medicine
[
] 기타 (
)
Apcdd1 for epithelial rearrangements in mice molar development
Sanjiv Neupane, Wern-Joo Sohn, Gi-Jeong Gwon, Youngkyun Lee, Jae-Young Kim
Department of Biochemistry, School of Dentistry, Kyungpook National University
After the genome wide screening, a molar tooth forming gene, Apcdd1 (Adenomatosis
polyposis coli down-regulated 1), was selected and evaluated expression pattern in mice
tooth development using RT-qPCR and in situ hybridizations. At E13.5 distinctive expression
pattern of Apcdd1 was detected in condensed mesenchyme and at E14.5, Apcdd1 was
observed in the enamel knot (EK). In vitro organ cultivation using Apcdd1 antisense
oliodeoxynucleotides (AS-ODN) was employed at E13 for 2 days to define the
developmental function of Apcdd1. After knocking down of Apcdd1, histogenesis and cellular
events such as cell adhesion, proliferation and apoptosis, were examined. These results
showed the altered morphogenesis of tooth germ with lower cell proliferation and changed
localization patterns of cell adhesion molecules after the Apcdd1 knocking down. Epithelial
rearrangement was also affected by Apcdd1 knocking down. Further, we evaluated altered
expression patterns of signaling molecules, related with EK, using RT-qPCR to understand
the precise signaling regulations of Apcdd1. In addition, renal transplantation was employed
to understand the detailed developmental function of Apcdd1 at cap stage in tooth crown
formation. Overall, we suggest that Apcdd1 would play crucial roles at cap stage in tooth
development.
References
[1] Shimomura et al., 2010. Apcdd1 is a novel Wnt inhibitor mutated in hereditary
hypotrichosis simplex. Nature 464(7291):1043-1047
[2] Takahashi et al., 2002. Isolation of a novel human gene, APCDD1, as a direct target of
the beta-catenin/T-cell factor 4 complex with probable involvement in colorectal
carcinogenesis. Cancer res. 62(20):5651-5656.
[3] Neupane et al., 2014. Developmental regulations of PERP in mice molar morphogenesis.
Cell Tissue Res. 358(1):109-121.
Keywords
Tooth crown development; Inner enamel epithelium; Signaling pathway; Cellular events
Picture
2014
MRC 성과발표회
Name :
Sanjiv Neupane
Affiliation :
Department of Biochemistry, School of Dentistry, Kyungpook
National University, 101 Dongin-dong 2-Ga, Joong-gu, Daegu 700422, Korea
Position :
Ph.D. candidate
E-mail :
[email protected]
Field of Expertise:
Developmental Biology
Education:
2004-2008
Master’s degree (Microbiology).
“Isolation, Identification and Plasmid Profiling of Multidrug
resistant Bacterial Pathogens Isolated from UTI Patients.”
Central Department of Microbiology, Tribhuvan University,
Kathmandu, Nepal
2001-2004
B. Sc. (Microbiology).
Department of Microbiology, Tri-Chandra Multiple Campus,
Kathmandu, Nepal
Professional Experience : (less than 5 experiences)
2012.9-Present
Research Assistant
Department of Biochemistry, School of Dentistry, Kyungpook
National University, Daegu, Korea
2011.3-2012.8
Teacher
Kalinchwok Higher Secondary School, Charikot, Dolakha, Nepal
2009.3-2009.8
Teacher
Kalinchwok Higher Secondary School, Charikot, Dolakha, Nepal
Selected Publications : (less than 5 papers)
1. Neupane S, Sohn WJ, Rijal G, Lee YJ, Lee S, Yamamoto H, An CH, Cho SW, Lee Y,
Shin HI, Kwon TY, Kim JY. Developmental regulations of Perp in mice
molarmorphogenesis. Cell and Tissue Research. 2014;358(1):109-121.
2. Baral P, Neupane S, Shrestha B, Ghimire KR, Marasini BP, Lekhak B. Clinical and
microbiological
observational
study
on
AmpC
β-lactamase-producing
Enterobacteriaceae in a hospital of Nepal. The Brazilian Journal of Infectious
Diseases. 2013; 17(2): 256-259.
3. Baral P, Neupane S, Marasini BP, Ghimire KR, Lekhak B, Shrestha B. High prevalence
of multidrug resistance in bacterial uropathogens from Kathmandu, Nepal. BMC
Research Notes. 2012; 5:38 * The first two authors contributed equally to this work.