Download Poietics™ hematopoietic progenitor growth medium

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Document # TS-CC-323-4 06/11
© 2011 Lonza Walkersville, Inc.
Poietics™ hematopoietic progenitor growth medium
HPGM™
Erythroid progenitors
PT-3926
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Introduction
HPGM™-hematopoietic progenitor growth medium
(catalog number PT-3926) is a serum-free medium
containing only human proteins that supports the
proliferation of human hematopoietic progenitor cells
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(CD34 cells). CD34 cells can be stimulated to
undergo a limited amount of proliferation and still
maintain a relatively undifferentiated phenotype
when grown in this medium.
Additionally, HPGM™ can be used as the growth
medium to support the differentiation of
hematopoietic progenitor cells, such as erythroid
progenitors, shown below.
CD36 cells (2C-250 or 2G-250) should be
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established in culture at a density of 2 x 10 to
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1 x 10 cells per ml of HPGM™ supplemented with
EPO (3 U/ml), SCF (25 ng/ml), IL-3 (10 ng/ml), and
IL-6 (10 ng/ml). After 5 to 7 days in culture in
HPGM™, glycophorin A and hemoglobin expression
will be observed in greater than 80% of the cells. A
small portion of the population can be expected to
continue to mature, will express glycophorin A in the
absence of CD71 expression, and will actually
enucleate.
Hematopoietic progenitors
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CD34 cells should be established in culture at a
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density of 2 x 10 to 2 x 10 cells per ml of HPGM™
and supplemented with cytokines - e.g. SCF (25
ng/ml), TPO (50 ng/ml), and FL (50 ng/ml). After 7
days in culture in HPGM™ the total cell population
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should expand by 10 to 20-fold and the CD34 cells
should expand by 5 to 8-fold. Other cytokine
combinations can be used with varying results.
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CD34 cells cultured in HPGM™
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or its subsidiaries.
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CD36 cells cultured in HPGM™
When placing an order or for scientific support,
please refer to the product numbers and
descriptions listed above. For a complete listing of
all Poietics™ products, refer to the Lonza website or
our current catalog. To obtain a catalog, additional
information or scientific support you may contact
Lonza by web, e-mail, telephone, fax or mail.
THESE PRODUCTS ARE FOR RESEARCH USE ONLY. Not
approved for human or veterinary use, for application to humans
or animals, or for use in clinical or in vitro procedures.
WARNING: CLONETICS™ AND POIETICS™ PRODUCTS
CONTAIN HUMAN SOURCE MATERIAL, TREAT AS
POTENTIALLY INFECTIOUS. Each donor is tested and found
non-reactive by an FDA approved method for the presence of
HIV-I, hepatitis B virus and hepatitis C virus. Where donor testing
is not possible, cell products are tested for the presence of viral
nucleic acid from HIV, hepatitis B virus, and hepatitis C virus.
Testing can not offer complete assurance that HIV-1, hepatitis B
virus, and hepatitis C virus are absent. All human sourced
products should be handled at the biological safety level 2 to
minimize exposure of potentially infectious products, as
recommended in the CDC-NIH manual, Biosafety in
Microbiological and Biomedical Laboratories, 5th edition. If you
require further information, please contact your site safety officer
or scientific support.
All trademarks herein are marks of Lonza Group
or its subsidiaries.