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Transcript
A bHLH Transcription Factor Regulates Anther Development and Dehiscence in Rice Rajeev Ranjan1, Reema Khurana2, Akhilesh K. Tyagi1, 2 1. National Institute of Plant Genome Research, New Delhi, India 2. Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, India Anther development and its dehiscence are important for the reproductive success of the flowering plants. bHLH class of transcription factors are known to regulate several plant growth and development processes. Here, we are describing cellular and molecular functions of a bHLH transcription factor during anther development process in rice. Previously, by using microarray, qRT-PCR and promoter-reporter assay in transgenic system we reported OsbHLH142 as an anther specific gene in rice. Expression analysis of OsbHLH142 transcripts through qPCR and its protein profiling through immune-blot and immunolocalization during different stages of anther development showed delayed accumulation of its protein compared to transcripts. Overexpression of OsbHLH142 in transgenic rice results in complete male sterility owing to indehisced anthers as well as production of non-viable, shrunken and abnormal shaped pollen. Electron microscopic and paraffin based tissue section analysis revealed detailed reason for these defects. RNA-Seq based transcriptome analysis of early and mature transgenics anthers has identified direct/indirect target of OsbHLH142 associated with anther functions. Moreover, through yeast two-hybrid and BiFC assays we have identified some OsbHLH142 interacting proteins which gives more insight on its molecular functions. Therefore, this study has explored novel functions of OsbHLH142 in rice anthers which will essentially help in the control of male fertility in crop plants for the hybrid seed production.