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Soil Series Changes in Mineral Soil C and N after a Thirty-year Interval L. Chris Kiser, J. Michael Kelly, and P. Alan Mays Virginia Tech Department of Forestry and Tennessee Valley Authority based on bootstrap analysis. Error bars represent bootstrap derived 95% confidence interval. For a comparison to be significant (*), confidence interval values must be either strictly positive or strictly negative. 1976 to 2006 based on bootstrap analysis. Error bars represent bootstrap derived 95% confidence interval. For a comparison to be significant (*), confidence interval values must be either strictly positive or strictly negative. 4.0 94 hectare aggrading oak forest 3.0 73 permanent sampling points Comparison of 1976 and 2006 mineral soil C concentration in the 0 to 10 cm depth Archived samples from 1976 re-analyzed along with samples from 2006 with Vario MAX CNS analyzer 2.0 Mean difference C g kg Methods Clifty: fine-silty, mixed, mesic Fluventic Dystrochrept - upland stream courses Sequoia: clayey, mixed, mesic Typic Hapludult - gently sloping uplands Wallen/Ramsey: loamy-skeletal, siliceous, mesic Typic Dystrochrept - loamy, siliceous, mesic Lithic Dystrochrept - steep side-slopes – Kalmia latifolia L. understory 0.0 * * -1.0 * * * * Clarkrange -2.0 Lily Wallen/Ramsey -3.0 Clifty Atkins -5.0 -6.0 1.0 Beersheba 0.5 0.0 Clarkrange tax * -0.5 * Clarkrange * * Sequoia -1.0 Lily -1.5 -2.0 Wallen/Ramsey -2.5 -3.0 Clifty Atkins -3.5 Soil Series Soil Series Fig.2 - Mean difference in mineral soil total-N concentration in g kg-1 from 1976 to 2006 based on bootstrap analysis. Error bars represent bootstrap derived 95% confidence interval. For a comparison to be significant (*), confidence interval values must be either strictly positive or strictly negative. Conclusions Changes in C and N were distinct to each of the 8 soil series. Increases in mineral soil C were associated with increases in the C/total-N ratio and decreases in mineral soil C were associated with decreases in the ratio of C/total-N. 0.3 Mean difference total-N g kg Clarkrange: fine-silty, siliceous, mesic Typic Fragiudult - broad, smooth plateaus and hilltops Sequoia Although both increases and decreases in mineral soil C -1 Beersheba and Lily: fine-loamy, siliceous, mesic Typic Hapludult - gentle slopes, narrow ridges, and benches Clarkrange tax 1.0 -4.0 Soil Series Atkins: fine-loamy, mixed, mesic Typic Fluvaquent - floodplains Beersheba -1 established at the intersections of a 100 by 100 m grid 1.5 Mean difference C/total-N ratio g kg /g kg State Park on the Cumberland Plateau in central Tennessee Fig.3 - Mean difference in mineral soil C/total-N ratio in g kg-1/g kg-1 from -1 Located within Fall Creek Falls Fig.1 - Mean difference in mineral soil C concentration in g kg-1 from 1976 to 2006 -1 Camp Branch Watershed Wallen/Ramsey 0.2 Beersheba Sequoia 0.1 -0.1 Clarkrange tax Clifty Atkins * * * Clarkrange -0.2 Soil Series Lily were observed after a 30-year interval, mineral soil C storage in Atkins, Clifty, and Wallen/Ramsey soils was greater than or equal to the remaining soil series in both 1976 and 2006 suggesting mineral soil C storage will be greater in these soils over the long term (mesic sites and sites with Kalmia latifolia L. understory). * Acknowledgements: Funding for this research was provided by the National Council for Air and Stream Improvement, Inc. (NCASI), the Tennessee Valley Authority (TVA), and Virginia Tech.