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National 5 Biology – Cell Biology Unit – Success Criteria Success Criteria – Cell Biology - National 5 6. I know that a mitochondrion is the Colour the box at the side of each objective: site of aerobic respiration in a cell. RED – I don’t know much about this or am confused by 7. I understand that extremely active it. cells such as sperm cells, muscle cells or AMBER – I know a bit about this but do not feel I liver cells will have many mitochondria know it well. to provide energy. GREEN – I am very confident that I know this 8. I can identify a chloroplast in a 1. Cell Structure 1. I can identify a cell wall in a diagram. 2. I Know that the function of a cell wall is to provide the cell with support (and stop the cell bursting). 3. I can identify a cell membrane in a diagram. 4. I know that the function of a cell membrane is to control entry and exit of materials to the cell. 5. I can identify a mitochondrion in a diagram. diagram. 9. I know that a chloroplast contains chlorophyll and is the site of photosynthesis in a cell. 10. I can identify the cell cytoplasm in a diagram. 11. I know that the cell cytoplasm is the site of all chemical reactions in the cell. 12. I can identify the vacuole in a diagram. National 5 Biology – Cell Biology Unit – Success Criteria 13. I know that the vacuole contains cell sap 22. I can list all of the organelles found in an (water and solutes) and controls water animal cell. balance in a plant cell. 23. I can list all of the organelles found in a 14. I can identify a nucleus in a diagram. plant cell. 15. I know that the nucleus controls all 24. I can list all of the organelles found in a cellular activity. fungal cell. 16. I can identify a ribosome in a diagram. 25. I can list all of the organelles found in a 17. I know that the ribosome is the site of protein synthesis in a cell. 18. I know that bacterial cells do not have a nucleus. 19. I know that bacterial cells have rings of genetic material called plasmids. 20. I understand that a plant cell wall is made of cellulose fibres. 21. I know that the structure of a cell wall in bacterial and fungal cells is different to the cell wall found in plant cells. bacterial cell. 26. I can identify a cell as being either bacterial, fungal, animal or plant in a diagram. National 5 Biology – Cell Biology Unit – Success Criteria 2. Transport across cell membranes 1. I can identify the cell membrane in a diagram. 2. I know that the cell membrane is made up of phospholipids and protein. 8. I know that osmosis is the movement of water molecules from an area of high concentration to a lower water concentration through a selectively permeable membrane. 9. I understand that animal cells burst when 3. I understand that the cell membrane is placed in a solution with a high water described as being selectively permeable. concentration. 4. I know that passive transport is the 10. I know that plant cells become turgid movement of molecules down a when placed in a solution with a high water concentration gradient. concentration and are prevented from 5. I understand that passive transport does not require energy. 6. I can list diffusion and osmosis as examples of passive transport. 7. I know that diffusion is the movement of molecules down a concentration gradient. bursting by their cell wall. 11. I know that animal cells shrink and plant cells become plasmolysed, when placed in a solution with a low water concentration. 12. I understand that active transport is the movement of molecules and ions against the concentration gradient (low concentration to high). National 5 Biology – Cell Biology Unit – Success Criteria 13. I know that active transport requires energy (provided by respiration). 5. I can describe the technique used to grow cell cultures on an appropriate growth 14. I understand that proteins in the cell media e.g. broths and agars. membrane act as carrier molecules to transfer 6. I can define the meaning of ‘aseptic ions or molecules across the membrane by active transport. 3. Producing new cells 1. I can state that a diploid cell contains 2 matching sets of chromosomes. 2. I know that a diploid cell divides in a process known as mitosis to maintain the diploid complement. 3. I can describe the sequence of events in mitosis including the involvement of the equator and spindle fibres (names of phases not needed). 4. I know that in early mitosis chromosomes make an extra copy of themselves called chromatids which are held together by a centromere. techniques’ (using sterile conditions). 7. I know how aseptic conditions are achieved. 8. I can state that the growth of cell cultures requires aseptic techniques. 9. I understand that all factors affecting growth, such as pH, temperature, oxygen and nutrient availability must be controlled when culturing cells. 10. I know that mitosis is required by all organisms for growth and repair of cells. National 5 Biology – Cell Biology Unit – Success Criteria 4. DNA and the production of proteins 1. I can describe DNA as a double stranded structure held together complementary base pairs. 2. I know that DNA carries the genetic information for making proteins. 3. I understand that DNA is made up of 4 bases, Adenine (A), Thymine (T), Cytosine (C) and Guanine (G). 4. I know that A always pairs with T, and C always pairs with G. 5. I know that the base sequence determines the amino acid sequence in a protein. 6. I understand that the sequence of amino acids in a protein determines the shape and function of the protein. 7. I understand that mRNA carries a complementary copy of the code from the DNA in the nucleus, to the ribosome where the protein is made. 5. Proteins and Enzymes 1. I can give examples of the functions of proteins (structural, enzymes, hormones, antibodies and receptors). 2. I understand that enzymes function as biological catalysts. 3. I know that enzymes are made by all living cells. 4. I can state that enzymes speed up the rate of all biochemical reactions, and remain unchanged by the reaction. 5. I know that the shape of the active site in an enzyme is complementary to 1 specific substrate. 6. I know that enzymes have optimum (best) conditions (ph and temperature) in which they work most effectively. 7. I understand that enzymes become denatured (active site changes shape) slowing down the rate of the reaction, out with the optimum pH or when the temperature is too high. National 5 Biology – Cell Biology Unit – Success Criteria 8. I know that enzyme action results in the 6. Genetic Engineering formation of a product. 1. I know that genetic information can be 9. I understand that enzymes can be involved transferred from one cell to another naturally in synthesis (build-up) or degradation (break- or by genetic engineering. down) reactions. 2. I know the stages of genetic engineering, to 10. I can give examples of enzyme reactions. include: Identify the section of DNA that contains the required gene from the source chromosome. Extract the required gene using enzymes. Extract plasmid from vector/bacterial cell using enzymes. Insert required gene into vector/bacterial plasmid using enzymes. Insert plasmid into host cell. Grow modified cells to produce a genetically modified organism. National 5 Biology – Cell Biology Unit – Success Criteria 7. Photosynthesis 1. I know that photosynthesis is a twostage process. 2. I can state that the first stage is the light reaction. 3. I understand that in the light reaction light energy from the sun is trapped by chlorophyll (in the chloroplast) and is 7. I can state that the second stage of photosynthesis is called carbon fixation. 8. I know that carbon fixation is a series of enzyme controlled reactions, which use hydrogen and ATP (produced in the first stage), with carbon dioxide to produce sugar. 9. I can state that photosynthesis occurs in the chloroplast. converted into chemical energy (in the 10. I know that the sugar made in form of ATP). photosynthesis can be used for respiration, 4. I know that water is split to produce hydrogen and oxygen. 5. I understand that hydrogen attaches to a hydrogen acceptor molecule and is carried to the second stage. 6. I can state that excess oxygen diffuses from the cell as a by-product. converted into starch (storage carbohydrate) or cellulose (structural carbohydrate). 11. I understand that there are factors (light intensity, carbon dioxide concentration and temperature) which can limit the rate of photosynthesis. 12. I understand the effect of limiting factors when presented on a graph. National 5 Biology – Cell Biology Unit – Success Criteria 8. Respiration 1. I know that respiration is a series of 6. I know that respiration occurs in two enzyme controlled reactions where chemical stages. energy is released from glucose. 2. I understand that ATP is an energy carrier made from ADP and inorganic phosphate. 3. I can state that the energy released from the breakdown of glucose is used to regenerate ATP. 4. I know that the chemical energy stored in ATP can be released by breaking it down to ADP and inorganic phosphate. 5. I can list examples of cellular activities the energy from ATP is used for (muscle contraction, cell division, protein synthesis and transmission of nerve impulses). 6. I know that ATP can be regenerated during respiration. 7. I can state that the first stage of respiration occurs in the cell cytoplasm. 8. I understand that glucose is broken down into pyruvate in the first stage. 9. I know that if oxygen is available (aerobic conditions) pyruvate is broken down into carbon dioxide and water. 10. I can state that in aerobic conditions there are 38ATP synthesised from the breakdown of one molecule of glucose. 11. I know that the second stage of aerobic respiration occurs in the mitochondria. 12. I understand that when oxygen is not present fermentation occurs. 13. I understand that in fermentation glucose is still broken down into pyruvate. 14. I know that during fermentation in animal cells pyruvate is converted into lactic acid. 15. I know that in fermentation in plant and yeast cells, pyruvate is converted to ethanol/alcohol and carbon dioxide. 16. I understand that the process of fermentation continues in the cytoplasm. 17. I know that the breakdown of glucose via the fermentation pathway yields only 2ATP in animal, plant and yeast cells. 18. I know that respirometers are used to measure the rate of respiration.