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Product datasheet Recombinant Human Glutathione S Transferase theta 1 protein ab117201 Overview Product name Recombinant Human Glutathione S Transferase theta 1 protein Protein length Full length protein Description Nature Recombinant Source Escherichia coli Amino Acid Sequence Accession P30711 Species Human Sequence MRGSHHHHHH KDRWGSHMGL NDIPFELRIV VPALKDGDFT WYPQDLQARA LWHKVMFPVF LQLLEDKFLQ LMHPVGAGCQ EDLFQEAHEV Molecular weight 32 kDa including tags Amino acids 1 to 240 Tags His tag N-Terminus Additional sequence information GSTT1 Human Recombinant fused with 37 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 277 amino acids (1-240 a.a.). GMASMTGGQQ ELYLDLLSQP DLIKGQHLSD LTESVAILLY RVDEYLAWQH LGEPVSPQTL NKAFLTGPHI VFEGRPKLAT ILKAKDFPPA MGRDLYDDDD CRAVYIFAKK ACAQVNPLKK LTRKYKVPDY TTLRRSCLRA AATLAELDVT SLADLVAITE WRQRVEAAVG DPTIKQKLMP WVLAMIR Specifications Our Abpromise guarantee covers the use of ab117201 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Applications SDS-PAGE Purity > 95 % SDS-PAGE. ab117201 is purified by proprietary chromatographic techniques. Form 1 Form Liquid Additional notes although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freezethaw cycles. Preparation and Storage Stability and Storage Shipped at 4°C. Please see notes section. pH: 8.00 Constituents: 10% Glycerol, 0.32% Tris HCl General Info Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide. Tissue specificity Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in Clara cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles. Sequence similarities Belongs to the GST superfamily. Theta family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain. Cellular localization Cytoplasm. Please note: All products are "FOR RESEARCH USE ONLYAND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 2