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39. During transcription, RNA polymerase
holoenzyme binds to a gene promoter and
assumes a saddle-like structure. What is its
DNA-binding sequence?
A) TATA
B) TTAA
C) AATT
D) CACC
40. During transcription, if the nucleotide
sequence of the DNA strand that is being coded
is ATACG, then the nucleotide sequence in the
mRNA would be
A) TCTGG
B) UAUGC
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C) UATGC
D) TATGC
41. One strand of DNA has the following sequence
of nucleotide 3’ ATTCGCTAT 5’ so the other
strand of DNA has
A) 5’ TAAGCGATA 3’ B) 3’ TAAGCGATA 5’
C) 5’ GACGCGATA 3’ D) 3’ GACGCGATA 5’
42. PCR and Restriction Fragment Length
Polymorphism are the methods for
A) DNA sequencing
B) Genetic fingerprinting
C) Study of enzymes D) Genetic transformation
Central dogma of protein synthesis is..
MOLECULAR BIOLOGY
1. If the total amount of adenine and thymine
in a double-stranded DNA is 45%, the
amount of guanine in this DNA will be
1) 45% 2) 55% 3) 22.5% 4) 27.5%
Choose the suitable answer
A) Only 1
B) 1 & 2
C) 2 & 3
D) only 4
2. Repressor proteins are coded by .......
genes.
I) Structural
II) Operator
III) Promoter
IV) Regulator
Choose the correct answer from the above
statements :
A ) Only I
B) II and III
C) Only IV
D) I, II and III
3. During replication of DNA, Okazaki
fragments are formed in the direction of
A) 3'--➠ 5'
B) 5'-➠ 3'
C) 5'--➠5'
D) 3'--➠3'
4. Central dogma of protein synthesis is
A) DNA --->RNA --->Protein
B) DNA --->DNA ---> Protein
C) RNA ---> DNA --> Protein
D) DNA ---> protein ---> RNA
5. DNA is a polymer made of ?
A) Amino acids
B) Fats
C) Sugars
D) Nucleotides
Choose the suitable answer
A) Only 1
B) 1 & 3
C) Only 3
D) only 4
6. The organelles in the cell where protein
synthesis occurs ........
1) Ribosomes 2) Endoplasmic reticulum
3) Nucleus
4 ) mRNA
Select the suitable answer
A) Only 1
B) 1 & 3
C) Only 3
D) 1,2,3 & 4
7. HIV is classified as a retrovirus because
its genetic information is carried in
I) DNA instead of RNA II) DNA
III ) RNA instead of DNA
IV) Protein coat
Choose the correct answer from the above
statements
A) Only I
B) II and III
C) Only III
D) I,II,III and IV
8. The primary control of gene expression
takes place at the level of
A) Translation
B) Transcription
C) DNA-replication
D) None of the above
9. Okazaki is known for his contribution to
the understanding of
I) Transcription
II) Translation
III) DNA replication
IV) Mutation
Pick up the right answer
A) Only I
B) II and III
C) Only III
D) I, II &III
10. What is not true for genetic code?
A) It is unambiguous
B) A codon in mRNA is read in a noncontiguous fashion
C) It is nearly universal D)It is degenerate
11. DNA sequence that code for protein are
known as
A) Introns
B) Exons
C) Control region
D) Intervening sequences
12. DNA is made of two chains that twist
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about one another in the shape of a .......
A) Broken ladder
B) Straight ladder
C) Straight spiral
D) Double helical
13. ....... occurs in RNA instead of the
nitrogenous base thymine found in DNA.
A) Uracil
B) Cytosine
C) Guanine
D) Ribose
14. Chromosome is made up of
A).DNA + pectin
B) RNA + DNA
C) DNA + Histone
D) Only histone
Pick up the right answer
A) Only I
B) II and III
C) Only III
D) II &IV
15. The region of tRNA which consists of
three bases complementary to mRNA
codon is
A) Codon
B) Intron
C) Anticodon
D) Interferon
16. A DNA segment which serves as a kind of
“on-off switch” for transcription is a/an
A) Regulator gene
B) Operator
C) Promoter
D) Structural gene
17. Mitochondria are semi autonomous as
they possess
A) DNA
B) DNA + RNA
C) DNA + RNA Ribosomes D) Protein
18. RNA has the sugar ....... instead of
deoxyribose.
A) Fructose
B) Ribose
C) Sucrose
D) Deoxyribose
19. There is no DNA in
A) Hair root
B) An enucleated ovum
C) Mature RBCs
D) A mature spermatozoan
20. The Segments of DNA, capable of
moving from one location to another are
A) Introns
B) Transposons
C) Exons
D) Operons
21. Match the followings:
A) tRNA
1) Linking of amino acids
B) mRNA
2) Transfer of genetic
information
C) rRNA
3) Nucleolar organising
region
D) Peptidyl Transferase 4) Transfer of
amino acid from
cytoplasm of ribosome
A) A = 4, B = 2, C = 3, D = 1
B) A = 1, B = 4, C = 3, D = 2
C) A = 1, B = 2, C = 3, D = 4
D) A = 1, B = 3, C = 2, D = 4
22. ....... catalyzes the formation of RNA on
a DNA template and capable of binding
to a promoter.
A) DNA polymerase
B) Helicase
C) DNA lipase
D) RNA polymerase
23. Gel electrophoresis is used for
A) Isolation of DNA molecule
B) Cutting of DNA into fragments
C) Separation of DNA fragments according
to their size
D) Construction of recombinant DNA by
joining with cloning vectors
24. The anti-parallel nature of DNA refers to
A) Its charged phosphate groups
B) The formation of hydrogen bonds
between bases from opposite strands
C) The opposite direction of the two strands
D) The pairing of bases on one strand with
bases on the other strand
25. What is antisense technology?
A) RNA polymerase producing DNA
B) A cell displaying a foreign antigen used
for synthesis of antigens
C) Production of somaclonal variants in
tissue cultures
D) When a piece of RNA that is
complementary in sequence is used to stop
expression of a specific gene
26. Beadle and Tatum showed that each
kind of mutant bread mould they
studied lacked specific enzyme. Their
experiments demonstrated that
A) Cells need specific enzymes in order to
function
B) Genes are made of DNA
C) Enzymes are required to repair damage
D) Genes carry information for making
proteins
27. Where is ribosomal RNA synthesized?
I) Nucleolus
II) Mitochondrion
III) Ribosome
IV) Vacuole
Pick up the right answer
A) Only I
B) II and III
C) I & III
D) III & IV
28.Which of the following is structural
subunit of DNA?
A) Protein
B) Carbohydrate
C) RNA
D) Nucleotides
29. The site of translation (protein synthesis)
is..
A) Mitochondria
B) Ribosomes
C) Nucleus
D) None of these
30. Removal of introns and joining the exons
in a defined order in a transcription unit
is called..
I) Capping
II ) Splicing
III ) Tailing
IV) Transformation
Select the right answer
A) Only I
B) Only II
C) Only III
D) I, II &III
31. The segment of DNA which acts as the
instrumental manual for the synthesis of
the protein is..
A) Nucleoside
B) Nucleotide
C) Ribose
D) Gene
32. Which of the followings are cancercausing genes?
A) Carcinogens
B) Mutagens
C) Metastases
D) Oncogenes
33. What are those structures that appear as
'beads-on-string' in the chromosomes
when viewed under electron microscope?
A) Base pairs
B) Genes
C) Nucleotides
D) Nucleosomes
34. The sections of a structural genes that
are expressed by
A) Interferons
B) Neurons
C) Introns
D) Exons
35. Which one of the following techniques
made it possible to genetically engineer
living organisms?
A) Heavier isotope labelling
B) Hybridization
C) Recombinant DNA techniques
D) X-ray diffraction
36. Molecular scissors which cut DNA at
specific site is
A) Pectinase
B) Polymerase
C) Restriction endonuclease
D) Ligase
37. Balbiani rings are the sites of
A) DNA replication
B) RNA and protein synthesis
C) Synthesis of lipids
D) Synthesis of polysaccharides
38. The genetic material of Rabies virus is
A) Double stranded RNA
B) Single stranded RNA
C) Double stranded DNA
D) ssDNA
Answer Keys
1. D
7. C
13. A
19. C
25. D
31. D
37. B
2. C
3. B
8. B
9. C
14.C 15.C
20. B 21. A
26. D 27. A
32. D 33. D
38. B 39. A
4. A
10. B
16. B
22. D
28. D
34. D
40.B
5. D
11. B
17. C
23. C
29. B
35. C
41. A
6. A
12. D
18. B
24. C
30. B
36. C
42. B