Download 3201-100361 - BiosPacific

PRODUCT SPECIFICATIONS
SHEET
Date: 2013-02-13
Note: For research use only.
P R OD UC T N AM E
:
Anti-Testosterone 3201 SPTN-5
PRODUCT SPECIFICITY
:
Testosterone
PRODUCT CODE
:
100361
PRODUCT BUFFER
:
50 mM Na-citrate, pH 6.0, 0.9 % NaCl, 0.1 % NaN3 as a preservative.
SHELF LIFE AND STORAGE
:
Shelf life unspecified, storage at 2-8 oC
AN AL YTE DES CRIP TION
:
Testosterone is a steroid hormone from the androgen group and is primarily secreted
in the testes of males and the ovaries of females, but small amounts also by the
adrenal glands. It is the principal male sex hormone and an anabolic steroid. In
men, testosterone plays a key role in the development of male reproductive tissues
as well as promoting secondary sexual characteristics. In addition, testosterone is
essential for health and well-being. Adult male body produces about ten times more
testosterone than an adult female body, but females are more sensitive to the
hormone.
P AR AM E TERS TES TED F ROM E AC H L O T
P R OD UC T APPE AR AN C E
:
Clear liquid, may turn opaque during storage
PRODUCT CONCENTRATION
:
5.0 mg/ml (+/- 10 %)
PRODUCT ACTIVITY
:
80 – 120 % compared to reference in an IFMA-test
IE F- R AN G E
:
N/D
PURITY
:
≥ 95 %
P AR AM E TERS DE TE RM IN ED O NL Y D U RIN G PRO D UC T R& D PH AS E
C L ASS AN D S U B CL AS S
:
IgG1
ASSOCIATION CONSTANT
:
9 x 105
DISSOCIATION CONSTANT
:
3 x 10-4
AF FI NI TY CO NS T AN T
:
KA = 3.0 x 109 1/M; KD = 3.3 x 10-10 M (= 0.33 nM)
D E TERM IN ATI O N M E THO D
:
SPR analysis (Biacore)
ANTIGEN
:
Testosterone
C R OSS - RE AC TI VI TIES
:
0.007 % DHEAS
7.9 %
5alpha-DHT
2.2 %
Androstenedione
Oy Medix Biochemica Ab
Asematie 13
FI-02700 Kauniainen
Finland
Telephone
+358-9-547 680
Fax
+358-9-505 3441
Email
[email protected]
Public website
www.medixbiochemica.com
EPITOPE
:
N/A
EPITOPE GROUP
:
Two antibodies binding to the same, or closely located epitopes, belong to the same
group and hence cannot be used as a pair in a sandwich assay. Epitope group
numbering does not give any detailed information where the epitope is located.
P AI R REC OMM END ATI ON S
:
SOLID
L AB E L
N/A
Please note that pair recommendations are based on results obtained in our
laboratory. Equally good results may be obtained using other pairs and therefore
these recommendations should be taken only as a directive.
:
P R OD UC T S TAB I LI TY
Please note that the shelf life given on
page one is based on real time stability
testing at +2-8 oC in the product buffer.
TEM PER ATU R E , D AYS
RESULT
-70 oC, 21 days
-20 oC, 21 days
+4 oC, 21 days
+25 oC, 21 days
+35 oC, 7 days
+35 oC, 21 days
+45 oC, 3 days
+45 oC, 7 days
N/D
N/D
N/D
N/D
N/D
N/D
N/D
N/D
Stability testing is performed in the product buffer to see whether different
temperatures affect the antigen binding, charge or composition of the antibody. The
maximum duration of the test is 21 days, except for the +45 oC only 7 days.
p H, 1 4 D AYS , +4o C
RESULT
5.0
N/D
6.0
N/D
7.0
N/D
8.0
N/D
Stability testing is performed to see whether pH affects the antigen binding, charge or
composition of the antibody during 14 days at +4 oC.
M IS CELL AN E OUS
:
Coupling to carrier protein at position 3 on steroid ring for immunization.
REFERENCES
:
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Legal disclaimer
MedixMAB monoclonal antibodies meet their specifications if transported, stored and used according to the instructions for use. See also separate Notes on use and storage of monoclonal antibodies. If not otherwise
agreed in writing all products are sold under General Delivery Terms. Other terms may apply if MedixMAB monoclonal antibodies are purchased from an official distributor of Medix Biochemica products. MedixMAB is a
registered trademark of Medix Biochemica and may not be used or reproduced without Medix Biochemica’s written permission. Further information on products and methods is available from medixMAB website.
Oy Medix Biochemica Ab
Asematie 13
FI-02700 Kauniainen
Finland
Telephone
+358-9-547 680
Fax
+358-9-505 3441
Email
[email protected]
Public website
www.medixbiochemica.com