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Chapter 10.1 MENDEL’S LAWS OF HEREDITY Who is Gregor Mendel? A monk in an Austrian monastary Carried out the first important studies of heredity First to succeed in predicting how traits are passed from parents to offspring Bred garden pea plants to study inheritance Why Mendel Chose Pea Plants They are genetically simple Mendel could study one trait at a time They reproduce sexually He could control breeding Mendel chose which plants to cross & studied one trait at a time They grow quickly Breeding Plants Reproductive Parts: Male = Stamen Female = Pistil Pollination = pollen from the anther is transferred to stigma Pollination can be controlled Traits observed •Flower color •Stem length •Seed color •Seed shape •Flower Position •Pod color •Pod shape Monohybrid Crosses Mendel carefully chose purebred (true-breeding) pea plants. Monohybrid crosses look at one trait at a time Example: flower color Crossing Pea Plants: 1. Mendel crossed purpleflowered plants with whiteflowered plants 2. Mendel planted the seeds, then allowed the F1 plants to self-fertilize 3. The resulting offspring F2 showed a 3:1 ratio of purple flowers Terminology P = parental generation F1 = first Filial generation F2 = second Filial generation Rule of Unit Factors Organisms have 2 factors that control each trait one from each parent Alleles = different forms of a gene Example: height may be tall or short; peas may be yellow or green, round or wrinkled. Rule of Dominance Dominant = the trait that is observed whenever it is present Shown as CAPTAL letters In pea plants, Tall is dominant written as T Recessive = the trait that is hidden if a dominant trait is present Shown as lower-case letters In pea plants, Short is recessive written as t Mendel’s 2 laws 1. Law of Segregation Each parent has 2 alleles that separate (segregate) during meiosis Gametes form random pairs during fertilization 2. Law of Independent Assortment Genes for different traits are inherited independently of one another Genotypes & Phenotypes Phenotype = the way an organism looks and behaves Usually a description Example: Plant height = tall or short Genotype = the genetic combination for an organism Usually the combination of alleles Example: TT or Tt both produce tall plants, tt produces a short plant You can’t always identify the genotype from the phenotype Genotypes Homozygous = two like alleles (TT or tt) True-breeding plants are generally homozygous Heterozygous = two different alleles (Tt) Crosses Monohybrid Cross = a cross involving one trait Dihybrid cross = a cross involving two different traits Parents: True-breeding Round, Yellow peas & Wrinkled, Green peas F1 generation = all Round, Yellow peas F2 generation – F1 generation self-pollinated to produce a mixture of offspring Yellow, round; Yellow, wrinkled; Green, round; Green, wrinkled The F2 phenotype ratio was 9:3:3:1 Punnett Squares Created by Reginald Punnett in 1905 Used as a tool to predict all of the possible outcomes of a genetic cross Monohybrid Cross Punnett Square is 2 x 2 Steps: set up the square do the cross determine offspring genotypes determine offspring phenotypes DRAW THIS Genotype Ratios Genotype looks at all of the possible genetic combinations To determine ratios, look at each pair inside of the square: TT = homozygous dominant (tall) 2. Tt = heterozygous 3. tt = homozygous recessive (short) 1. Genotypes: 1 TT, 2 Tt, 1 tt Genotype Ratio = 1:2:1 Phenotype Ratios Phenotype looks at the trait that will be expressed Doesn’t account for “hidden” recessive alleles Any offspring combination that has the dominant allele will show the dominant trait. Phenotypes: 3 Tall, 1 short Ratio = 3:1 Dihybrid Cross Punnett Square is 4 x 4 Steps: 1. 2. 3. 4. 5. Figure out the gametes set up the square do the cross determine offspring genotypes determine offspring phenotypes Probability The chance of getting a certain outcome Example: A coin has two sides – heads & tails The probability of getting heads is ½ or 1:2 Probability in Punnett Squares: Tt x Tt The probability of getting a tall plant is ¾ or 75%