Download Pro versus eukaryotic cells_Lesson Plan

Prokaryotic vs. Eukaryotic Cells
BRIEF SUMMARY
Introductory Activity: Students will compare and contrast diagrams of an animal cell
with a bacterial cell using a Venn Diagram.
Notes: Students will take notes about the differences between prokaryotic and eukaryotic
cells, noting that both types can cause infection.
Lab/Analysis: Students will be asked to figure out which of their plates contain
prokaryotic cells and which contain eukaryotic cells. They will have to master streaking
techniques (which should be introduced prior to this lab). They will also be charged with
analyzing their plates for either bacterial or fungal growth. Finally, they will have to make
the connection that if there is growth on a plate containing ampicillin, that the plate must
contain fungus (a eukaryotic organism) whereas, if there is growth on a plate containing
cyclohexamide, the plate must contain bacteria (a prokaryotic organism).
GRADE LEVEL
Grades 9-11
LEARNING OBJECTIVES
Students will:
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Compare and contrast prokaryotic and eukaryotic cells
Analyze petri dishes for the presence of prokaryotic and eukaryotic cells
STANDARDS
Essential Standards (Biology) 1.1.2
Compare prokaryotic and eukaryotic cells in terms of their general structures (plasma
membrane and genetic material) and degree of complexity.
CLASS TIME REQUIRED
180 minutes (Two 90-minute class periods)
PRECEDENTS
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Knowledge of cells, organelles and membranes
Streaking techniques (see tutorial videos under additional resources)
MATERIALS AND TECHNOLOGY NEEDED
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LB agar
Cotton swabs
E.coli K-12
Ampicillin
Yeast
Cyclohexamide
Petri Dishes
TEACHER INSTRUCTIONS
DAY 1
Do Now: Students describe their experiences about the last time they were sick. You may
wish to utilize the included PowerPoint presentation to help facilitate discussion.
Engage: Students watch the trailer for the movie Contagion and discuss why people in
the movie get sick.
Explore: Teacher introduces the concept of a pathogen. Students take notes, then
individually answer questions about what is considered a pathogen.
Students then work in partners to compare/contrast two different types of cells.
Responses are shared with the class. An optional worksheet is provided.
Explain: Students take notes on Prokaryotic vs. Eukaryotic cells. Teacher gives
examples about how both kinds of cells can make us sick or keep us healthy.
Evaluate: Students answer a multiple choice exit ticket concerning the differences
between prokaryotic and eukaryotic cells.
DAY 2
Do Now:
3 organelles that are in eukaryotic cells but not prokaryotic cells
2 differences in prokaryotic and eukaryotic genetic material
1 difference in size between prokaryotic and eukaryotic cells
True or False: Partners are given cards with true or false statements about prokaryotic
and eukaryotic cells. They must organize them into true and false piles.
Lab:
Teacher Prep
• Teacher prepares two different types of LB agar; one with a cyclohexamide solution
and one with an ampicillin solution
• Teacher prepares a plate with E. coli K-12 bacteria (the “A” substance)
• Teacher prepares a plate with yeast in agar. (The “B” substance)
Student procedures are detailed in the attached lab.
ADDITIONAL RESOURCES
Preparing plates - https://www.addgene.org/plasmid-protocols/bacterial-plates/
Detailed Plate Streaking Tutorial: https://youtu.be/Ay2hhujTuvg
Simple Plate Streaking Tutorial:https://youtu.be/EqMz_jcd3us
AUTHOR
Sara Greenberg
This resource is made available under a Creative Commons
Attribution Noncommercial Share-alike license (CC BY-NC-SA 4.0)
and was funded in part through the NC Summer Ventures Fellows
program. Summer Ventures is a no-cost, state-funded program for
academically talented North Carolina students who aspire
to careers in science, technology, engineering, and mathematics.
https://www.ncssm.edu/summerventures