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Molecular Diagnostics Laboratory
Department of Pathology and Laboratory Medicine
What is Molecular Diagnostics?
Molecular diagnostic testing is used to diagnose and
monitor oncological and genetic diseases. It helps
determine risk and prognosis. By analyzing the individual's
genetic code and how their cells express their genes as
proteins, molecular diagnostics allows for personalized
medicine .
Molecular Team
How Do We Help Our Patients?
Bone Marrow Transplants: Molecular diagnostics plays a
large role in stem cell transplants by determining whose
cells are populating the bone marrow post transplant
(donor or recipient?) This difference helps the treatment
team determine the next steps for a successful transplant.
Oncology: Molecular testing can diagnose and help guide
therapy for patients with all varieties of cancer (lung,
colon, brain, skin, blood, etc.)
How Is DNA Analyzed?
DNA is first extracted from the blood, bone marrow, or
tissue samples. The polymerase chain reaction (PCR) is the
technique used for the amplification of DNA to make
millions of replicates of a target DNA sequence. Specific
mutations which can be associated with certain diseases
are detected once the DNA is amplified.
Genetics: There are certain genetic mutations that are risk
factors for bleeding and iron overload disorders. Molecular
diagnostics can help determine if the patient has the
mutation and the likelihood the patient will develop the
Top: Dr. Paul Rothberg, Bonnie Nuccie
Middle: Sharon, Todd, Nufatt, Paige
Bottom: Suni, Yanyun, Bill, John
Post PCR gel electrophoresis
DNA sequencing analysis
Real time PCR of the KRAS gene