Download Chicken embros

OIE Reference Laboratory Reports
Activities in 2011
Name of disease (or topic) for
which you are a designated OIE
Reference Laboratory:
Address of laboratory:
Highly pathogenic avian influenza and low
pathogenic avian influenza (poultry)
427 Maduan Street,
Harbin 150001,
PEOPLE’S REPUBLIC OF CHINA
Tel.:
(86) 45151997168
Fax:
(86) 451 51997166
e-mail address:
website:
[email protected]
http://hvri.ac.cn/
Name (including Title and
Position) of Head of Laboratory
(Responsible Official):
Hualan Chen
Name(including Title and
Position) of OIE Reference
Expert:
Hualan Chen
Name (including Title and
Position) of writer of this report
(if different from above):
Annual reports of OIE Reference Centres, 2011
1
Highly pathogenic avian influenza and low pathogenic avian influenza (poultry)
Part I: Summary of general activities related to the disease
1.
2.
Test(s) in use/or available for the specified disease/topic at your laboratory
Test
For
Specificity
Total
Hemagglutinin inhibition
Antibody
H5
10,989
Hemagglutinin inhibition
Antibody
H9
Hemagglutinin inhibition
Antibody
H7
Hemagglutinin inhibition
Antibody
H6
RT-PCR
Antigen
Group, H5, H9, H7
800 each
Chicken embros
Virus isolation
H5
47 (Virus)
Chicken embros
Virus isolation
H9
174 (Virus)
Chicken embros
Virus isolation
H6
77 (Virus)
10,989
10,989
10,989
Production and distribution of diagnostic reagents
Type of reagent
Amount supplied nationally
(including for own use)
Amount supplied to
other countries
RT-PCR Kits (H5)
390 kits (20samples/kit)
0
RT-PCR kit for H1N1 swine influenza
viruses
89 kits (50 samples/kit)
0
Antigen
36,800 ml
2500 ml
Antigsera
10,000ml
1200ml
Part II: Activities specifically related to the mandate
of OIE Reference Laboratories
3.
International harmonisation and standardisation of methods for diagnostic testing or the
production and testing of vaccines
a)
Establishment and maintenance of a network with other OIE Reference Laboratories
designated for the same pathogen or disease and organisation of regular inter-laboratory
proficiency testing to ensure comparability of results
 Not in 2011.
b)
Organisation of inter-laboratory proficiency testing with laboratories other than OIE
Reference Laboratories for the same pathogens and diseases to ensure equivalence of
results
 Not in 2011.
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Annual reports of OIE Reference Centres, 2011
Highly pathogenic avian influenza and low pathogenic avian influenza (poultry)
4.
Preparation and supply of international reference standards for diagnostic tests or vaccines
We provided 4,400ml antigen for H5 antibody detection and 1,640ml antisera for H5 antigen detection to Egypt in
2011.
5.
Research and development of new procedures for diagnosis and control
An H5 DNA vaccine has been developed and large scale field tests have been finished in 2010. In 2011, we
performed more studies to address the questions raised by the reviewing panel.
In 2011, we generated a recombinant duck enteritis virus (DEV) expressing the HA gene of H5N1 avian influenza
virus, ducks studies indicated that this recombinant virus could work as a bivalent vaccine against the challenge of
both lethal DEV and H5N1 virus in ducks (Liu J et al, 2011, Journal of Virology).So far, we have finished the both
laboratory and field tests of this vaccine.
In addition, on the basis of the epidemiology information of H5N1 influenza virus, the clade 2.3.2.1 viruses are the
dominative strains in the wild birds and poultry in southeast Asia, we developed new H5N1 inactivated vaccine
and recombinant NDV vaccine and finished all of the necessary animal studies of these vaccines. We have
submitted the proposal for the vaccine strain update to the Ministry of Agriculture in China.
6.
Collection, analysis and dissemination of epizootiological data relevant to international disease
control
We performed the final diagnosis of H5N1 disease outbreak in chickens in Tibet in 2011, and this information has
been reported to OIE through the Ministry of Agriculture.
We also collected total 11491 swab samples and 10989 sera samples from live birds markets, poultry farm and
slaughter house for isolation of influenza viruses and detection of H5 and H9 sera antibody. From these samples,
we isolated 443 avian influenza viruses, including 46 H5N1, 174 H9N2 and 77 H6 influenza viruses.
7.
Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the
pathogen and the disease concerned
We well maintained our bio-safety level 3 (P3) facilities for handling the H5N1 influenza viruses.
8.
Provision of consultant expertise to OIE or to OIE Member Countries
The expert organized “The 5th China-Japan Bilateral Avian Influenza Symposium” in Tokyo, Japan, from 23-25
January.
The expert attended the “China and Vietnam Forum on HPAI Risk Management and Control” in 8 – 9 March 2011,
Beijing China and provided consultant expertise on avian influenza vaccination.
The expert was invited to give a lecture in “5th Vaccine and ISV Annual Global Congress” in Seattle, USA, from
2-5 Oct, and provided consultant expertise on avian influenza vaccination.
The expert was invited to attend the “Influenza Risk Assessment Algorithm Meeting” organised by Centers for
Disease control and Prevention (CDC), from 27-29 Oct and provided consultant expertise on avian influenza risk
analysis.
9.
Provision of scientific and technical training to personnel from other OIE Member Countries
We provided a six-month-training to one Egypt scientist on avian influenza vaccine development and evaluation.
The training started on Dec.2.2010 and ended on June 1,2011.
Annual reports of OIE Reference Centres, 2011
3
Highly pathogenic avian influenza and low pathogenic avian influenza (poultry)
We provided a three-day-training to 13 Egypt scientists on diagnosis technology on avian influenza. The training
started on Jul.5.2011 and ended on Jul.7.2011.
We provided a one-week-training to 3 Sudan scientists on diagnosis technology on avian influenza. The training
started on Aug.1.2011 and ended on Aug.8.2011.
10. Provision of diagnostic testing facilities to other OIE Member Countries
Not in 2011.
11. Organisation of international scientific meetings on behalf of OIE or other international bodies
We organised “The 5th China-Japan bilateral symposium on influenza” in Tokyo from Jan 24-26, about 100
scientists participated the meeting. Organizers: Professors Yoshihiro Kawaoka, Hualan Chen, Fu Gao and Yuelong
Shu.
12. Participation in international scientific collaborative studies
Title of the study: Control of emerging and re-emerging infectious diseases.
Objective: To understand the evolution of H5N1 influenza viruses; to explore the genetic basis of the host range
and virulence of influenza viruses; and to develop novel vaccines for avian influenza.
Project partners: Professor Yoshihiro Kawaoka in the University of Tokyo, Japan
Time frame: 2006-2010
13. Publication and dissemination of information relevant to the work of OIE (including list of
scientific publications, internet publishing activities, presentations at international conferences)
Jianzhong Shi, Jianzhong Shi, Zhiyuan Wen, Jing Guo, Ying Zhang, Guohua Deng, Yuelong Shu, Dayan Wang,
Yongping Jiang, Yoshihiro Kawaoka, Zhigao Bu, Hualan Chen. Protective efficacy of an H1N1 cold-adapted live
vaccine against the 2009 pandemic H1N1, seasonal H1N1, and H5N1 influenza viruses in mice. Antiviral
Research, accepted, 2012.
Hongmei Bao, Xiurong Wang, Yuhui Zhao, Y Sun, Yanbing Li, Yongzhong Xiong, Hualan Chen. Development
of a reverse transcription loop-mediated isothermal amplification method for the rapid detection of avian influenza
virus subtype H7. Journal of Virological Methods, 2011, accepted.
Jinxiong Liu, Pucheng Chen, Yongping Jiang, Li Wu, Xianying Zeng, Guobin Tian, Jinying Ge, Yoshihiro
Kawaoka, Zhigao Bu, and Hualan Chen. A Duck Enteritis Virus-vectored Bivalent Live Vaccine Provides Fast
and Complete Protection against H5N1 Avian Influenza Virus in Ducks. Journal of Virology, 2011, 85 (21):
10989-10998.
Jinying Ge, Xijun Wang, Lihong Tao, Zhiyuan Wen, Na Feng, Songtao Yang, Xianzhu Xia, Chinglai Yang,
Hualan Chen, and Zhigao Bu. Newcastle Disease Virus-Vectored Rabies Vaccine Is Safe, Highly Immunogenic,
and Provides Long-Lasting Protection in Dogs and Cats. Journal of Virology, 2011, 85 (16): 8241-8252.
Huanliang Yang, Yan Chen, Jianzhong Shi, Jing Guo, Xiaoguang Xin, Jian Zhang, Dayan Wang, Yuelong Shu,
Chuanling Qiao, Hualan Chen. Reassortant H1N1 Influenza Virus Vaccines Protect Pigs against Pandemic H1N1
Influenza Virus and H1N2 Swine Influenza Virus Challenge. Veterinary Microbiology, 2011, 152 (3-4): 229-234
Shimizu K, Li C, Muramoto Y, Yamada S, Arikawa J, Chen H, Kawaoka Y. The NP and M segments of H5N1
influenza viruses are responsible for dominance in embryonated eggs. J Gen Virol. 2011. 92: 1645-1649
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Annual reports of OIE Reference Centres, 2011
Highly pathogenic avian influenza and low pathogenic avian influenza (poultry)
Songhua Shan, Yongping Jiang, Zhigao Bu, Trevor Ellis, Xianying Zeng, John Edwards, Guobin Tian, Yanbing
Li, Jinying Ge, Hualan Chen*. Stan Fenwick. Strategies for improving the efficacy of a H6 subtype avian
influenza DNA vaccine in chickens. Journal of Virological Methods, 2011, 173(2): 220-6.
Yanbing Li, Liling Liu, Yi Zhang, Zhenhua Duan, Guobin Tian, Xianying Zeng, Jianzhong Shi, Licheng Zhang,
Hualan Chen*. New Avian Influenza Virus (H5N1) in Wild Birds, Qinghai, China. Emerging Infectious
Disease, 2011, 17 (2), 265-267
Jiasheng Song, Huapeng Feng, Jing Xu, Dongming Zhao, Jianzhong Shi, Yanbing Li, Guohua Deng, Yongping
Jiang, Xuyong Li, Pengyang Zhu, Yuntao Guan, Zhigao Bu, Yoshihiro Kawaoka, Hualan Chen*. The PA protein
directly contributes to the virulence of H5N1 avian influenza viruses in domestic ducks. Journal of Virology.
2011, 85(5), 2180-2188.
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Annual reports of OIE Reference Centres, 2011
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