Download Data/hora: 06/05/2017 06:27:48 Biblioteca(s): Embrapa Gado de

Data/hora: 08/06/2017 22:40:04
Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia.
Data corrente: 14/02/2017
Data da última atualização: 14/02/2017
Autoria: WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; REIS, D. R. de L.; FERNANDES, L. E.;
PEIXOTO, M. G. C. D.; MACHADO, M. A.; VIANA, J. H. M.
Afiliação: S. Wohlres-Viana, UFJF; E. K. N. Arashiro, UFJF; DANIELE RIBEIRO DE LIMA REIS
FAZA, CNPGL; L. E. Fernandes; MARIA GABRIELA CAMPOLINA D PEIXOTO,
CNPGL; MARCO ANTONIO MACHADO, CNPGL; JOAO HENRIQUE MOREIRA
VIANA, Cenargen.
Título: Polymorphisms and alternative splicing of the luteinizing hormone receptor of dairy
cattle.
Ano de publicação: 2016
Fonte/Imprenta: Genetics and Molecular Research, v. 15, n. 2, 2016.
Páginas: 12 p.
Idioma: Inglês
Conteúdo: Abstract
The aim of this study was to screen for variability in the luteinizing
hormone/choriogonadotropin receptor (LHCGR) and to determine the occurrence of
LHCGR mRNA isoforms in two dairy breeds of cattle. Granulosa cells from dominant
ovarian follicles were recovered from 16 Gir and 16 Holstein cows, and total RNA was
extracted. Complementary DNA was synthesized and PCR was used to generate
amplicons for sequencing. Chromatograms were evaluated, and multiple sequences were
aligned and analyzed for the presence of polymorphisms, allele frequency, polymorphic
information content (PIC), and Hardy-Weinberg equilibrium (HWE). Twentyone single
nucleotide polymorphisms (SNPs) were identified in LH receptor mRNA. Seventeen
SNPs were identified in Gir cattle (seven exclusively), and 14 were found in Holstein
cattle (four exclusively). Seven of the 21 polymorphisms found did not alter which amino
acid was translated. Eight SNPs caused a change to an amino acid in a different chemical
group. Classification of SNPs according to PIC values identified 12 as being highly
informative in Gir cattle and five in Holstein. Eight SNPs deviated from HWE in Gir
compared with 11 in Holstein, and eight in both breeds. Two isoforms were also
identified, one in exon 1, which lacks 30 nucleotides beginning at position 118, and the
other in exon 10. Taken together, these data show that LHCGR in dairy cattle breeds has
a high frequency of polymorphism and exists in multiple isoforms resulting from
alternative splicing.
Palavras-Chave: LHCGR isoforms; Reproduction; Zebu.
Categoria do assunto: L Ciência Animal e Produtos de Origem Animal
URL: http://ainfo.cnptia.embrapa.br/digital/bitstream/item/155636/1/Cnpgl-2016-GMR-Polylm
orphisms.pdf
Registro original: Embrapa Gado de Leite (CNPGL)
Exemplares: CENARGEN - (36666-1) - UPC - SP - PP - SP 21040 - - SP 21040 - DIS
CNPGL - (23289-1) - UPC - SP - PP - SP 7191 - - SP 7191 - DIS