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Daily Lesson Plan Presented by: Bonnie Zobel Subject: Biology Topic: How skeletal muscles work Grade: 11-12 or AP Allocated Time: lesson planned for a 1 hr class Student Population: 30 students State Standards: 3.3.12.A- Explain the relationship between structure and function at all levels of organization 3.3.12.B- Analyze the chemical and structural basis of living organisms Goal for Understanding: By learning how skeletal muscles work and contract, students can further understand how their bodies work. Instructional Objective: Students will be able to explain to someone how muscles contract and understand the terms myosin, actin, the different zones, and how ATP, calcium, and sodium come into play. Student Behaviors Sources of Evidence Criteria for Evaluation Students will label a diagram with skeletal muscle components. Students will keep this worksheet in their notebooks. Teacher labeled diagram in order for students to compare. Students will take notes on lecture on muscle movement. Students will need these notes in order to do the assignment. Just make sure enough time is given for students to complete the notes. Students will either create a comic strip, write a short story, compose a song, or create a play to describe skeletal muscle action. Students have to share with the class the final project. This is more of a Friday assignment to be due Monday. Use a rubric to grade (yes or no questions). So many yes’s are equivalent to a grade. Teaching to the Objective Estimated Teaching to the Objective: Time: Introduction/Motivation/Prior Knowledge Students have already been exposed to muscle locations in the human body, and different types of muscles. Differentiation: Required for each section 5-10 min Review some of the muscle locations by pointing to a muscle on a student and asking what it is called. Then review the differences between skeletal and cardiac muscle. (voluntary/involuntary) (striated/smooth) Ask students what is the powerhouse of the cell? ATP- adenosine triphosphate and tell them that this is a vital link to how muscles work. Also ask them why striated muscle is “striated”? It has filaments. They will be able to explain different parts of these filaments and the different filaments by the end of the class. Developmental Activities 15 min 5 min Have students write down new vocabulary words including: 1.)myosin- makes up the thick filaments of a muscle and have heads to help with contraction 2.)actin- makes up the thin filaments of muscle and look like two strands of pearls twisted around each other, contain binding sites for myosin heads 3.)muscle fiber- another name for muscle cell due to its elongated shape 4.)fascicle- a small bundle of 10-100 muscle fibers 5.)sarcoplasm- the cytoplasm of a muscle fiber 6.)myofibril- a threadlike structure that extends along the length of a muscle fiber, consisting mainly of thick and thin filaments 7.)sacromere- the basic functional contractile unit of a myofibril 8.)A band- the darker middle area of the sarcomere which extends the entire length of the thick filaments 9.)tropomyosin- one of the two regulatory proteins that make up part of the thin filament; covers the myosin-binding sites on actin 10.)troponin- one of the two regulatory proteins that make up part of the thin filament; binds to calcium ions and moves tropomyosin away from the myosin-binding sites on actin 11.)crossbridge- this forms when the myosin heads attach to actin during muscle contraction 12.)tendon- a cord of dense regular connective tissue composed of parallel bundles of collagen fibers that attaches muscle to bone Show picture of skeletal muscle cell (using picture from http://staff.fcps.net/cverdecc/Adv%20A&P/Notes/Muscle%20Unit/Musc le%20Anatomy/anatomy_review.htm) Using the word bank, have students use book, new vocab and prior Provide students who need more time hard copies of these words. Require them to copy the words but give them extra time and if necessary the paper to take home to finish. For students who may have a hard time seeing the screen, hand out a copy of the picture for a closer visual reference knowledge of cells to label the cell with you, this allows them to see the somewhat big picture of how skeletal muscles work because the cell is the biggest component to the actions. Explain to students before going into too many details that contracting a muscle is like pulling a rope. Have a volunteer come to the front of the class and perform the following activity: 5 min 1. Grab the rope with both hands, arms extended. 2. Loosen your grip with one hand, let's say the left hand, and maintain your grip with the right. 3. With your right hand holding the rope, change your right arm's shape to shorten its reach and pull the rope toward you. 4. Grab the rope with your extended left hand and release your right hand's grip. 5. Change your left arm's shape to shorten it and pull the rope, returning your right arm to its original extended position so it can grab the rope. 6. Repeat steps 2 through 5, alternating arms, until you finish. (from: http://health.howstuffworks.com/muscle2.htm) Then use powerpoint slides to demonstrate the process and different actions and timing of muscle contraction. Set up slides in the following order (use these types of terms but find pictures for each slide): 25 min 1. Nerve Impulse: Action potentials arrive at synaptic end bulb -- the axon terminal. (Whole structure = motor unit.) 1a. Calcium ions diffuse into synaptic bulb through voltage-gated calcium channels. 2. Acetylcholine released by vesicles through exocytosis into synaptic cleft, 3. and then it binds to ACh receptors on the sarcolemma. 4. Na+ ion enters through these ion channels (and K+ leaves). 5. Na+ entrance sets up an action potential on the sarcolemma. The sarcolemma is depolarized. Muscle fiber is excited. 6. The action potential travels to the T-tubules Give every student a copy of the slides with plenty of room to take notes, on the slides use pictures, words, movies…whatever is best for reaching students. Also, mix in a slide to let them take a breather (stand up and stretch their muscles) 7. where the terminal cisternae release Ca2+ into the sarcoplasm. 8. Ca2+ binds to the troponin, causing a shift of the tropomyosin. 9. The myosin binding sites (on the actin) are exposed . 10. - 13. Power stroke follows. (10.) ATP molecule which is bound to the myosin head is hydrolyzed to ADP + Pi. It (ADP) remains for the moment on the myosin head. The energy released by this process causes the myosin head to swivel. (Actually, it straightens out.) (11). Activated myosin head will now bind to the actin, forming a cross bridge. This formation is a spontaneous reaction. (12.) In the process, the ADP + Pi is released and the head flexes (to a bent, lower energy position), causing the thin filament to be pulled along the myosin. (13.) Myosin head remains bound until a new ATP molecule binds to it. (13. cont'd.) The next (new) ATP causes the head to be released from the actin, thus allowing the head to repositioned for another power stroke. Sliding filament theory = shortening of the sarcomere in an "all-or-none" pattern. Refer to rope climbing analogy. REMEMBER THAT ONLY THE SARCOMERE SHORTENS, NOT THE PROTEIN FIBERS. 14. Stimulus stops. 15. ACh is destroyed by AchEsterase or diffuses throughout the synaptic cleft. Membrane is no longer permeable to Na+. ACh also diffuses away. 16. Ca2+ transported (active, ATP used) back into the sarcoplasmic reticulum. 17. Low [Ca2+] in sarcoplasm causes Ca2+ to leave the troponin. 18. The tropomyosin shifts around to cover the myosin binding sites. The sarcomere will lengthen as the filaments slide back to their original position. (from: http://kcfac.kilgore.cc.tx.us/mobleypageap1/unit%203/18_steps_muscle_contraction.htm) Closure 5-10 min Ask students if they have any questions and explain to them that you understand it is a lot of information and is somewhat difficult to digest. We will review again tomorrow. Ask them a few easy questions like: What is the difference between actin and myosin? What is the difference between troponin and tryptomyosin? What ions are vital in the process? What molecule is essential? Show the video linked if time permits: http://www.youtube.com/watch?v=ren_IQPOhJc&feature=related Follow-Up: Make sure students have copied the vocabulary words, and are all given a copy of the powerpoint slides with room for notes. Also, if time does not permit for the video, assign it for homework for a student review. Tell students for homework they also must bring in one question about skeletal muscle contraction. Materials: Transparency of muscle picture with blank spots for labeling, a list of vocabulary words with definitions for students to copy (one class set), a rope, a powerpoint of the 18 steps, a link to the video, a computer and way to project the powerpoint onto a board for visual. Resources and References: http://staff.fcps.net/cverdecc/Adv%20A&P/Notes/Muscle%20Unit/Muscle%20Anatomy/anatom y_review.htm -great pictures of skeletal muscle http://www.youtube.com/watch?v=ren_IQPOhJc&feature=related - good review video http://health.howstuffworks.com/muscle2.htm - good information about muscle contraction, along with interactive video and pictures http://quizlet.com/1049341/biol-121-chp-10-vocab-muscle-tissue-flash-cards/ - vocabulary words http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/M/Muscles.html - good information http://kcfac.kilgore.cc.tx.us/mobleypageap1/unit%203/18_steps_muscle_contraction.htm- steps in the process http://people.eku.edu/ritchisong/301notes3.htm great website with tons of extra information Technology: Computer, overhead projector