Download BIOTECH FALL FINAL review16

BIOTECH FALL FINAL REVIEW SHEETDO ON A SEPARATE SHEET OF PAPER – NOT IN NOTEBOOK!
1. Identify the functions of the following parts of the cell: cell membrane, nucleus, ribosome,
mitochondria, cell wall.
2. Explain the function of the following organic macromolecules: glucose, carbohydrates, lipids, and
proteins.
3. Explain what an indicator is and what the following indicators test for: benedicts, iodine, CuSO4 +
NaOH(buiret). Identify what color is the positive test for each indicator.
4. Identify different volume measurement instruments and explain when to use each one.
5. Identify different mass measurement instruments and explain when to use each one.
6. Draw the three micropipet windows and label the place values for each one.
7. Give a volume measurement example for each micropipet window.
8. Define concentration. Give 3 examples of concentration units.
9. Identify the mass/volume solution equation.
10. Do 2 sample mass volume problems.
11. Identify the % solution equation.
12. Do 2 sample % problems.
13. Calculate the molecular mass of HCl, NaOH.
14. Identify the molarity solution equation.
15. Do 2 sample molarity problems using the molecular mass from #14.
16. Identify the dilution equation.
17. Do 2 sample dilution problems.
18. Draw an E.Coli growth curve and label its parts.
19. Identify what is grown on the following types of media: potato agar, blood agar, malt agar.
20. Explain the difference between broth and agar.
21. Explain what SDS is and how it used in our DNA and protein extraction labs.
22. Draw a simple DNA gel and explain what is occurring using the word electrophoresis. Identify
who/what is involved in terms of DNA.
23. Draw an adenine nucleotide and label its parts.
24. What is the central dogma of biology?
25. What are 3 differences between DNA and RNA?
26. Explain what an RFLP is how it is used to diagnose sickle cell anemia.
27. Draw a simple sickle cell gel and explain what the bands represent.
28. What are RFLP’s used for?
29. Explain the role of restriction enzymes in an RFLP. Be sure to understand the difference
between sticky and blunt ends.
30. Explain what Alu is.
31. Draw a simple gel of Alu results and identify genotypes based on a gel.
32. What are 2 similarities and 2 differences between an RFLP and the Alu PCR?
33. What part of the amino acid caused the differences in structure?
34. Identify the different levels of protein structure.
BIOTECH FALL FINAL REVIEW SHEETDO ON A SEPARATE SHEET OF PAPER – NOT IN NOTEBOOK!
1. Identify the functions of the following parts of the cell: cell membrane, nucleus, ribosome,
mitochondria, cell wall.
2. Explain the function of the following organic macromolecules: glucose, carbohydrates, lipids, and
proteins.
3. Explain what an indicator is and what the following indicators test for: benedicts, iodine, CuSO4 +
NaOH(buiret). Identify what color is the positive test for each indicator.
4. Identify different volume measurement instruments and explain when to use each one.
5. Identify different mass measurement instruments and explain when to use each one.
6. Draw the three micropipet windows and label the place values for each one.
7. Give a volume measurement example for each micropipet window.
8. Define concentration. Give 3 examples of concentration units.
9. Identify the mass/volume solution equation.
10. Do 2 sample mass volume problems.
11. Identify the % solution equation.
12. Do 2 sample % problems.
13. Calculate the molecular mass of HCl, NaOH.
14. Identify the molarity solution equation.
15. Do 2 sample molarity problems using the molecular mass from #14.
16. Identify the dilution equation.
17. Do 2 sample dilution problems.
18. Draw an E.Coli growth curve and label its parts.
19. Identify what is grown on the following types of media: potato agar, blood agar, malt agar.
20. Explain the difference between broth and agar.
21. Explain what SDS is and how it used in our DNA and protein extraction labs.
22. Draw a simple DNA gel and explain what is occurring using the word electrophoresis. Identify
who/what is involved in terms of DNA.
23. Draw an adenine nucleotide and label its parts.
24. What is the central dogma of biology?
25. What are 3 differences between DNA and RNA?
26. Explain what an RFLP is how it is used to diagnose sickle cell anemia.
27. Draw a simple sickle cell gel and explain what the bands represent.
28. What are RFLP’s used for?
29. Explain the role of restriction enzymes in an RFLP. Be sure to understand the difference
between sticky and blunt ends.
30. Explain what Alu is.
31. Draw a simple gel of Alu results and identify genotypes based on a gel.
32. What are 2 similarities and 2 differences between an RFLP and the Alu PCR?
33. What part of the amino acid caused the differences in structure?
34. Identify the different levels of protein structure.