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Transcript
Technology on
“Alternative analytical technology for Soil
Nutrient analysis through image
processing Chromatograms”
Shri AMM Murugappa Chettiar Research Centre
(MCRC),
Taramani, Chennai 600 113,
Tamil Nadu, India.
i
Title
: Alternative analytical technology for Soil
Nutrient analysis through image processing
Chromatograms
Author
: Dr. K. Perumal, M.Sc.,Ph.D
Principal Scientist
Shri AMM Murugappa Chettiar Research
Centre, Taramani, Chennai 600 113.
Email:[email protected]
Web: amm-mcrc.org
Technical Assistants
: Mr. J. Arunkumar, Research Fellow
Ms. Sowmya selvaraj, Research Fellow
Financial Support
: Department of Science & Technology
Technology Division
Technology Bhawan, New Mehrauli Road
New Delhi 110 016
&
Murugappa Group, Dare House, Chennai
Publisher
:Shri AMM Murugappa Chettiar Research
Centre, Taramani, Chennai 600 113.
Email:[email protected]
Web: amm-mcrc.org
Year of Publish
: May 2008
Printed by
:
ii
Content
Importance Of Soil Testing
1
Why Alternative Analytical Technology (AAT) For Soil Testing
1
AAT For Testing Soil Nutrients
1
AAT Process
2
Quantification Of Soil Nutrients
2
Accuracy
3
Technology Protection
3
Advantages Of AAT
3
Disadvantages Of AAT
3
Soil Test Reports By AAT
4
Infrastructure
8
Cost For Soil Testing By AAT
9
Who Can Benefit
9
Technology Transfer
9
Contact
9
Acknowledgment
iii
Importance of soil testing: Indian agriculture occupies an eminent position in
global cultivation of rice, wheat, sugarcane, pulses and vegetables. Sustainable
agriculture should carefully consider maintaining and improving the existing soil
structure and innate productive capacity of this soil. Mere application of chemical
fertilizer and over working with soil without taking into consideration, the virgin
soil health (microorganism, humus organic matter etc) would in the long run
counterproductive. It is in this context, one must view the importance of soil
testing. It is the basic information for the farmer to decide, the extent of fertilizer
and farm yard manure to apply at various stages of the growth cycle of the
produce. Also such soil testing must provide sufficient information on trace
elements available in the virgin soil there by lead to a strategy of maintaining the
health of the soil over many years, and making up the depletion by adding micro
nutrient, to the soil by biological or mineral application.
Why alternative analytical technology (AAT) for soil testing:
There is no simple, cost effective and reliable technology available to determine
the level of organic carbon, humus, nitrogen, phosphorus, potassium and
micronutrients (10) in soil so as to provide the farmer the information he requires
for maintaining the health of the soil. The present methods that are available
require sophisticated, costly and equipment. Depending on the information
required, the cost of testing a single soil sample could vary between Rs. 250 to Rs.
1250 per soil).
AAT for testing soil nutrients: Shri AMM MCRC and IIT Madras has jointly
undertaken a project titled “Integrated Knowledge Systems on Soil Nutrient
Analysis through Image Processing of Chromatograms” funded by Department of
Science & Technology and Murugappa Group for a period of 3 years. More than
12,000 soil samples have been processed for circular Paper chromatograms, image
processing and case based reasoning. Software for image processing of soil
chromatogram so as to know the soil composition, fertilizer recommendation and
best suitable crop has been developed by us and was tested at various levels.
1
AAT Process:
Soil sampling and processing
Mixing of Soil in alkali solution
Digestion of soil solution at 125 rpm
Incubation at static condition
Spreading of photo reactive substance on Whatman No.1 filter paper
Drying of filter paper under dark condition
Application of Soil alkali extracts
Spread in the pre-treated dried filter paper
Drying of filter paper completely
Image development
Image scanning
Image processing
Case base reasoning
Printing soil test report
Getting advice on suitable crop and fertilizer application.
Quantification of Soil nutrients: The three primary nutrients Viz Nitrogen,
Phosphorus, and Potassium (kg/acre), three secondary nutrients Ca, Mg and S and
six micronutrients of Boron, Copper, Iron, Manganese, Molybdenum and zinc
(mg/kg or ppm), all making up a figure of 13 and organic carbon (%) and humus
(kg/acre) in the soil are being critically analyzed through the case based reasoning
2
system. In addition to macro and micro nutrients, pH and Ec are also determined
through AAT.
Accuracy: The new technology developed gives acceptable level of accuracy
(>90%) compared with conventional analytical methods.
Technology protection: The technology on alternative analytical technology for
soil testing is protected under patent application (652/CHE/2008).
Advantages of AAT: The advantages of this alternative analytical technology on
quantification of soil nutrients are simple, reliable, require no sophisticated
equipment, quick testing of soil nutrients (18 properties), advice on soil input
based on the test report, advice on best suitable crops, should help the farming
community and at an affordable cost.
Duration: The analysis and the test repot through AAT will be completed within
one day.
Disadvantages of AAT: Though AAT is simple and cost effective, the farmers
are still dependent on expertise available with experts.
3
Soil test reports by AAT
4
5
6
7
Infrastructure required for AAT (50 soils/day)
S.No Details
Units
Cost ( Rs)
Glassware & Materials
1
2
3
Conical flask (250ml capacity @ Rs 150 / flask
Watch glass @ Rs 7
Micropipette 1ml and 5ml @ Rs 5000/pipette
Equipment : Filter paper marking device
100
100
1 each
1
1,5000
700
10,000
5,000
4
5
6
Orbital Shaker (120 rpm)
Weigh balance
Chromatogram box @ Rs.20,000 / box containing temperature
and humidity controllers
Drying table ( 5” x 8”) (50 samples to dry)
Trays
Image processing facility
1
1
2
25,000
12,000
40,000
1
10
10,000
1,000
1
1
1
lot
13
Computer (2 GB RAM, 250 GB capacity)
Laser printer
Scanner
Miscellaneous
Overall Total
Building facility ( 20’x15’)* rented
50,000
10,000
10,000
10,000
1,91,500
3,000
14
Technology fee**
7
8
9
10
11
12
Rented
Grand total
* Available building or existing laboratory facility can be considered
** One time payment (Non exclusive basis), Technology fee includes technical training
on AAT to the licensee for 3 days for 2 person
8
Cost for Soil testing by AAT (50 soils /day)
Details
Chromatogram Image processing
image making using computer
(Rs / soil test) (Rs./ soil test
Distilled water (Rs 4 / 1000 ml (50 ml/soil)
Whatman No.1 Filter paper discs (2 sheets @ Rs 4.50 /sheet
0.20
9.00
Nil
Nil
AgNO3 ( 25gms @Rs 1500 (0.5% - 1 ml / soil)
0.10
Nil
Wick paper (3 x 3 cm, 2 wicks)
NaOH (500g @ Rs 125 (1%, 50 ml / soil)
0.70
0.15
Nil
Nil
Electricity charges for drying (800 lux,12 hrs)
0.40
0.00
Miscellaneous charges
2.00
0.00
Manpower
1. Technical Assistant (1). Rs 5000/month) 50 samples/day)
2. System Manager (1) Rs.20000 / 25 day (Rs. 800 / 50 soil
testing / day)
Total
Overall cost / soil
4.00
16.00
16.60
16.00
32.60
Who Can benefit: Soil science laboratories in universities, Agriculture colleges,
Research organizations, fertilizer companies, Government / Non governmental
organization working on agriculture.
Technology Transfer: The technology on AAT shall be transferred to national and
international entrepreneurs. Technological assistance is available in MCRC for settingup of laboratory on AAT for soil nutrients testing.
For Further Details, Please Contact
The Deputy Director (R&D and Admin)
Shri AMM Murugappa Chettiar Research Centre,
Taramani, Chennai 600 113
Phone: 044-22430 937
Fax; 044-22430369
Email: [email protected]
Acknowledgement:
We
gratefully
acknowledge
financial
support
(DST/TSG/MCRC/2003/76) from Department of Science & Technology, (Technology
Systems Programme), Technology Bhawan, New Mehrauli Road, New Delhi 110 016.
9