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Name: _____________________________ Date: ___________________________ Period: _______ DNA and DNA Technology Test Review Use the mRNA codon table to complete the missing information below. The first row has been completed to help you get started. Notice that the tRNA is included in this table. Be sure that you use the codon to determine the amino acid. DNA T A C C T A T G G C T G C T T mRNA A U G G A U A C C G A C G A A tRNA U A C C U A U G G C U G C U U Amino acid 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. START ASPARTATE THREONINE ASPARTATE GLUTAMATE Which codons do not code for an amino acid? ___THE FIRST ONE_______________ What is the purpose of the above codons? ___TO START THE CHAIN__________ Which codon is the “start” codon? __AUG_________________ What could happen if you made a mistake making the mRNA from the DNA? _________It would code for the wrong amino acid___________________________ What is it called when you make mRNA from DNA? ___transcription________ Where does this occur? ___nucleus_________________ What is it called when you match up tRNA with the mRNA? ____translation__________ Where does this occur? __ribosome___________ What are the three base pair sets called in mRNA? __codon_____________________ What are the three base pair sets called in tRNA? ________anticodon________________ What is the structure of DNA called? __Helix__________Is it single or Double stranded?_____double__________ List the 3 parts of a DNA nucleotide: _phosphate, base, deoxyribose sugar_____________ Which parts are on the outside of a DNA molecule? __phosphates and sugars________________________ Which parts are on the inside of a DNA molecule? _____bases_________________________________ List the 4 nitrogen base pairs in DNA: ____ATCG___________________________ What is the complimentary strand of DNA for ACCGTA? __TGGCAT_______________________ List the 3 parts of a RNA nucleotide: _phosphate, base, ribose sugar__________________________________ List the 4 nitrogen base pairs in RNA: ___AUCG______________________________ What is the complimentary mRNA strand for a DNA strand of ACCGTA? ____UGGCAU_______________ What types of bonds are between nitrogen bases in DNA? _Hydrogen________ Are these bonds weak or strong? _weak______Why is this important?_DNA has to unzip_____________ What types of bonds are between deoxyribose and phosphate molecules in DNA? _Covalent____________ Are these bonds weak or strong? ___strong_____________________ What types of bonds are between amino acids? ___peptide_________________________ What are changes (or glitches) in DNA called? ___mutations_________________________ What is the goal of replication? ____to copy DNA for mitosis___________________ What is the goal of transcription? ____to make mRNA__________ Where does it take place? __nucleus______ What is the goal of translation? ____use mRNA to make proteins_________ Where does it take place? _ribosome___ Which of these three processes is involved in protein synthesis? __Transcription and Translation____ Draw a picture of each of the three types of RNA and list their functions and locations: mRNA Picture: tRNA rRNA Name: _____________________________ Date: ___________________________ Period: _______ Function: Copy the DNA message Transfer the Amino Acids to the Ribosome Make the ribosome Location: Nucleus and ribosome Ribosome (cytoplasm) Ribosome(cytoplasm) How is the structure and function of DNA and RNA related? Different sugar, U vs. T, Single or double stranded , DNA cannot leave the nucleus 27. List the 3 steps of REPLICATION: DNA unzips Enzymes copy both sides of the DNA molecule Two DNA molecules that are half old and half new 28. List the steps of PROTEIN SYNTHESIS.See #25 and your notes sheet! 34. Label the parts of the picture below: Nucleus trna mrna an Cannot type in the blank but UUU should be labeled Anti-codon codon DNA Technology 1. What most likely happens when a mutation occurs? 2. Which type of mutation is the most detrimental? 3. Why are mutations in sex cells more detrimental? 4. Define and describe the process of Gel electrophoresis. What are the practical applications of it? 5. How and why do the fragments move through the gel? 6. Be able to interpret how many fragments will be produced with a particular enzyme. 7. Be able to interpret DNA fingerprinting samples. 8. Describe the cloning process and be able to recognize it. 9. What biomolecule is changed when exposed to radiation? 10. What are some practical uses of DNA technology? Name: _____________________________ Date: ___________________________ Period: _______ Name: _____________________________ Date: ___________________________ Period: _______ 1. 2. 3. 4. B is a ribosome D D D Recombinant DNA