Download Eötvös Loránd Science University Faculty of Sciences Department of

Eötvös Loránd Science University
Faculty of Sciences
Department of Chemistry
Core (required) / Core Elective /
Free Elective Course
COURSE OUTLINES
and Prerequisites
2013
Course Title: Basic Gene Technology
1.
Course Code
Semester
Grading
bv1c1bi1
5.
K
Credits / Language
Weekly
Hours
2
English
Major
Chemistry
BSc
2. Course Type:
lecture
3. Course Instructor and Department:
László Nyitray, Dept. Biochemistry, Institute of Biology
4. Course Instructor:
Name:
Title:
Department, Institution:
László Nyitray
Full Professor
Dept. Biochemistry, Biology
5. Course Requirements:
Fundamentals of Modern Biology
6. Course Prerequisites:
6.1. Prerequisites
6.2. Recommended
bv1c1bi2, Fundamentals of Modern Biology; kv1c1bk2, Biochemistry (parallel course)
7. Course Objectives:
The course intends to introduce students to the principles and applications of gene technology, that is recombinant DNS techniques, based on background knowledge of biochemistry and molecular biology. Our important goal is that the students understand the controversial and often misinterpreted concepts associated with gene technology such as cloning,
gene manipulation, GMO’s and other important applications of this technology.
8. Course Outline:
Topics Covered: Molecular biology processes. Terminology and history of gene technology. Molecular cloning and basic methods of gene technology. Polymerase chain reaction
(PCR; an in vitro DNA amplification method). Vectors and host cells used for cloninig.
Types of DNA libraries. DNA sequencing methods and genome projects. Basic bioinformatics. Site-specific in vitro mutagenesis methods. Methodological possibilities to produce
recombinant proteins. Fundamentals of protein engineering. Targeted gene modifications
and genetically modified organisms (GMOs). Basics of gene therapy.
8.1. Course Outline (Weekly)
1. Gene technology and molecular biology. Fundamentals of molecular biology. DNS
structure
2. Replication, transcription, translation. DNA repair.
3. Terminology and landmarks in gene technology. Basics of molecular cloning:, vectors,
inserts, ligation, amplification, selection.
4. DNA modifying enzymes and their application. Design of recombinant DNA constructs.
5. DNA introduction into cells (transformation, infection, electroporation). Electrophoresis
methods. Hybridization and DNA-chip techniques. Hybridization probes.
6. Polymerase chain reaction and its various applications.
7. Vector-host systems. Pro- and eukaryotic host cells. Plasmids, bacteriophages, phagmids, cosmids, artificial chromosomes (BAC, YAC), shuttle vectors.
8. Recombinant DNA libraries. Genomic and cDNA libraries. Screening of recombinant
clones.
9. DNA sequencing. Sanger enzymatic (chain termination) method. Fluorescence automatic DNA sequencers. New-generation DNA sequencing. Genome projects.
10. Introduction to bioinformatics. Molecular biology databases (GenBank, Uniprot, Ensembl, PDB). Sequence analysis: comparisons, alignments, predictions, sequence motifs
etc.
11. In vitro mutagenesis. Site-specific and random mutations, insertions, deletions.
12. Recombinant proteins. In vitro translation. Expression systems in pro- and eukaryotic
organism. Expression vectors. Fusion constructs.
13. Gene targeting: knock-out and knock-in experiments. Gene silencing. Transgenic microorganism , animals and plants.
14. Interaction and in vitro evolution techniques. Introduction to gene therapy. Summary
and future outlook.
9. Remarks:
10. Requirements:
10.1. Course Work:
Only students registered in the Neptun system can attend the lectures.
Attending of all classes and make written notes is highly recommended.
10.2. Exam Period
Study for written colloquium type exam.
11. Make Up Policy
12. Office Hours/Consultation:
Will be announced by course mail
13. Lecture Notes, Textbook, Resources, Recommended Reading:
Recombinant DNA: Genes and Genomes - A Short Course, 2007 Third Edition (Watson,
Recombinant DNA) by James D. Watson, Richard M. Meyers, Amy A. Caudy (ISBN10: 0-7167-2866-4)
14. Learning Strategy:
Studying the material each week during the semester and at least four days of preparation
for the exam.
15. Syllabus Written by (Name, Title, Department/Institution)
László Nyitray, Full Professor, Dept. Biochemistry, Institute of Biology
16. Enforcement Date:
September 1, 2014