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9.1 Manipulating DNA
9.1 Manipulating DNA

... Synthesize: How are restriction enzymes used in making restriction maps? Visual: If the purple fragment is farthest from the green in the original DNA strand, why is the yellow fragment farthest away from the green in the gel? View Restriction Enzyme ...
Unit 4
Unit 4

...  Genome may be Double-stranded DNA, Single-stranded DNA, Double-stranded RNA, Single-stranded RNA, Organized as single nucleic acid molecule, Either linear or circular, May have as few as 4 genes to as many as several hundred. Capsid has a Protein coat and Encloses viral genome. It’s shape may be R ...
Jeopardy - TeacherWeb
Jeopardy - TeacherWeb

... The allele of a disorder when the affected individual inherits it from unaffected parents. ...
(DNA, RNA, or DNA/RNA) Microinjection Service Form
(DNA, RNA, or DNA/RNA) Microinjection Service Form

... demonstrated successful in vivo target cleavage in mouse embryos in a limited number of experiments, not all targets may induce genome editing equally well, and so the GTTR cannot guarantee successful cleavage/editing. It is the responsibility of the investigator to confirm whether mutagenesis and/o ...
BI0034
BI0034

The Origins of Variation
The Origins of Variation

... e.g., mitochondria - endosymbiotic origin, evidence from cell membranes, gene structure, origin of replication, the fact that mitochondrial rRNAs are more similar to endosymbiotic bacterial (Rickettsia) rRNAs than to nuclear rRNAs of eukaryotes, and ongoing movement of genetic material from mitochon ...
11. Genetic engineering case study 1 - Human Insulin
11. Genetic engineering case study 1 - Human Insulin

... • Some cells are transferred onto agar that contains ampicillin, so only those that have taken up the plasmid with the gene to kill the bacteria will grow • Some cells from these colonies are transferred onto agar that has been made with tetracycline, so only those that have ten up a plasmid that do ...
Slide 1
Slide 1

... Most types of cell in an organism contain a complete copy of its genome. The organisation is quite complicated, but the simplest fact about any genome is that it is a collection of DNA sequences – long strings of the chemical ‘letters’ A, T, G and C (adenine, thymine, guanine and cytosine) in a part ...
DNA - Glen Ellyn School District 41
DNA - Glen Ellyn School District 41

... Both alleles together make up a gene that the offspring carries. The trait that the offspring has follows the instructions of the dominant allele ...
Final Review
Final Review

... Genetic information flows from a sequence of nucleotides in a gene to a sequence of amino acids in a protein. ● The enzyme RNA-polymerase reads the DNA molecule in the 3' to 5' direction and synthesizes complementary mRNA molecules that determine the order of amino acids in the polypeptide. ● In euk ...
Paper Plasmids Lab
Paper Plasmids Lab

... DNA molecules are pieces of DNA that have been reassembled from pieces taken from more than one source of DNA. A gene coding for particular protein is transferred into a host organism. The host multiplies, and produces the desired protein in volume. For example~ the gene that codes for the productio ...
Forensic DNA Analysis
Forensic DNA Analysis

... repeats of 9 to 80 base pairs (bp), total length is 500 to 23,000 bp, very specific due to length and repeats, testing is expensive and time-consuming, degrade in older DNA samples due to random breaking of DNA strands ...
H.S.A. REVIEW
H.S.A. REVIEW

chapter 6 vocabulary card sort
chapter 6 vocabulary card sort

... chromosome piece reattaches to the original chromosome but in a reverse ...
Ch. 8: Presentation Slides
Ch. 8: Presentation Slides

... The Genetics of Bacteria and Their Viruses ...
Daily Learning Targets
Daily Learning Targets

... These are the official learning targets for unit 5: Heredity. We will be covering all of these learning targets in class, and they will be used to construct our next exam. I may choose to break some of these learning targets down into smaller, more manageable steps if we need to. You must learn the ...
DNA Technology
DNA Technology

... not fully utilize their second X chromosome • This is so that females do not produce more proteins as a male of the same species ...
Ch_ 19_2
Ch_ 19_2

... adjust levels of enzymes for synthesis & digestion ...
No Slide Title
No Slide Title

... vertebrate-specific - so most domains are older than common ancestor of all animals - new ones are not “invented” very often • Many of these are concerned with defence/immunity and the nervous system • Most novelty is generated by new protein “architectures”, combining old domains in new ways (fig 4 ...
pGLO2011 Wilkes
pGLO2011 Wilkes

... plate. Be sure you can actually see that you have picked up a colony. Pick up the + DNA tube and immerse the loop into the transformation solution at the bottom of the tube. Be sure that you have actually transferred the colony to the tube. Spin the loop between your index finger and thumb until the ...
DNA Deoxyribonucleic Acid
DNA Deoxyribonucleic Acid

... THE PROCESS OF TRANSLATION ...
Recombinant DNA I
Recombinant DNA I

... by DNA Methylation • Addition of CH3 to selected C’s in DNA can inactivate genes, e.g. high levels are seen in inactivated X chromosome of female mammals. • Mammals have about 5% methylation. • Not essential in eukarotyes, since Drosophila has 0% methylation. • First observed in lac operon: methylat ...
goals - s3.amazonaws.com
goals - s3.amazonaws.com

... nucleotide sequence from human PV92 locus on chromosome 16. • The object is to create a personal DNA fingerprint that shows the presence(+) or absence (-) of the “Alu” DNA sequence on the paternal and maternal chromosomes. • We will use primers for the Alu sequence that will generate a 550 bp fragme ...
DNA Replication - OG
DNA Replication - OG

... cartilage and tendons ...
File - Schuette Science
File - Schuette Science

... •sections of your chromosome •made up of DNA ...
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Extrachromosomal DNA



Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.
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