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Sequential Elimination of Major-Effect Contributors Identifies
Sequential Elimination of Major-Effect Contributors Identifies

... gants. Segregants were analyzed for Htg by colony size assay at 41°. For the targeted backcross mapping strategy, segregants were selected such that they were Htg1 but contained the Htg allele of the previously identified QTL and were then backcrossed to S288c. Strain manipulations and gene deletio ...
Microbial Genetics - University of Montana
Microbial Genetics - University of Montana

... T-even and T-odd phage The T-even phages, T2, T4 and T6, are all related serologically and all have large genomes; T4 has a genome 168,895 bp in length The T-odd phages fall into three serological groups: T3 and T7 are related to each other but not to T1 or to T5, which are unrelated. The T7 genome ...
Genetic and Epigenetic Regulation in Age
Genetic and Epigenetic Regulation in Age

... the machinery of epigenetic regulation that is ultimately responsible for the gene expression regulation. The most common DNA methylation form is the 5¶ methylcytosine. It occurs predominantly in the symmetric CG context. Approximately 70% to 80% of CG dinucleotides of the genome are normally methyl ...
Evolution of a pseudo-control region in the mitochondrial genome of
Evolution of a pseudo-control region in the mitochondrial genome of

... might stay for longer and occasionally interbreed with B. oreophilus cannot be excluded. In the present study we tried to elucidate the unresolved phylogeny of B. buteo and its relatives in a molecular approach. Analyses of mitochondrial (mt) sequences of the genus Buteo have not yet been extensivel ...
Current Microbiology
Current Microbiology

... of unknown function (hoxE), is separated by at least 16 kb from the residual structural genes hoxUYH in an unusual way. With respect to the accessory genes, hoxW, hypA, B, and F are contiguous downstream of hoxH in A. nidulans [4], whereas they are scattered throughout the genome of Synechocystis [1 ...
Use of Recombinant Adenovirus for Metabolic Engineering of
Use of Recombinant Adenovirus for Metabolic Engineering of

... into freshly isolated rat islets of Langerhans. Infection of isolated islets with the AdCMV-PGal virus resulted in expression of P-galactosidase in islets for at least 21 days postinfection. Analysis of multiple islet sections showed that the recombinant virus transferred the P-galactosidase gene in ...
A RARE KEL17/KEL(IVS3+1G>A) COMPOUND HETEROZYGOUS
A RARE KEL17/KEL(IVS3+1G>A) COMPOUND HETEROZYGOUS

... Background: The Kell blood group system includes some of the most immunogenic antigens among blood a a groups know. Among them Kell(KEL1), Kp (KEL3), and Js (KEL6) are well known. The antithetic antigens KEL11/17 further contribute to this list. However, KEL17 is considered as very rare, with an app ...
Structure, expression and chromosomal location of the Oct
Structure, expression and chromosomal location of the Oct

... the relationship between Oct-4 and t 12 is to analyze the genomic structure and sequence of Oct-4 from the t 12 haplotype. A t w5 homozygous revertant ( t w5g unpublished) cosmid library was screened with the same Oct-4 probe to exclude trivial differences between the t- and wild-type sequences. A c ...
Complete comparative genomic analysis of two field isolates of
Complete comparative genomic analysis of two field isolates of

... (v90/4, 15?0–27?1 kb; v90/2, 15?0–28?3 kb). In this 12?1 kb region (7?7 % of the genome), there are 261 of the 521 point mutations, accounting for 50 % of the total nucleotide changes. Of the 261 point mutations, 82 cause nonsynonymous changes and only the chitinase and orf27 genes do not have any a ...
PDF - Blood Journal
PDF - Blood Journal

... these 2 loci. It is therefore interesting to determine the structure of the globin loci in more primitive vertebrates. Here, we describe the isolation of cDNAs encoding ␣- and ␤-globin from F rubripes. We have used these cDNAs to isolate the genomic loci of these genes. Surprisingly, the genes have ...
SNP Analysis of the PTC Gene Using PCR
SNP Analysis of the PTC Gene Using PCR

... Four nucleotide letters specify the genetic code: A (Adenine), C (Cytosine), T (Thymine), and G (Guanine). A point mutation occurs when one nucleotide is replaced by another nucleotide. For example when an A is replaced by a C, T or G (Figure 1). When such a mutation is present in at least 1% of the ...
Severe Perinatal Thrombosis in Double and Triple Heterozygous
Severe Perinatal Thrombosis in Double and Triple Heterozygous

... of this English family had also experienced thrombotic manifestations and/or low levels of both protein C and protein S (Fig 1, Table 1). 1.2 experienced several episodes of phlebitis while 11.6 suffered a deep vein thrombosis after surgery and has also experienced a pulmonary embolism. 111.2, the p ...
Navigating the NCBI Intructions
Navigating the NCBI Intructions

... c. With the exception of MT, the chromosomes of the human genome are in pairs. X and Y are a pair. Using this information and the information from your answer to Question 9A, how many pairs of chromosomes are in the human genome? 10. The Breast Cancer Susceptibility gene BRCA1 is on chromosome 17 in ...
Manipulating the Plasmodium Genome
Manipulating the Plasmodium Genome

... approaches (Hoffman et al., 1998; 2002). Comparative genomics will soon be possible as the genome sequence of more Plasmodium species and other Apicomplexa is completed, and will provide insights into the evolution of these protozoan parasites and adaptation to their hosts. To what extent will the s ...
here - FasterDB
here - FasterDB

... Figure 12. Comparison of PTB Binding Sites between Human exon 13 and its orthologous exon in the mouse genome (exon 15) among the WNK gene (A). CLIP-seq data for PTB at the exon level (B). Exon Arrays visualization when PTB is depleted in the Human (C) and Mouse (D). A. For each exon and 200 nucleot ...
A Single Gene Causes Both Male Sterility and
A Single Gene Causes Both Male Sterility and

... introgression hybrid males that inherit the P{w+; GA19777BOG} transgene show significantly female-biased progeny as compared with those of males that do not inherit the transgene (when crossed with Bogota white females). The two genotypes are identical except for the presence or absence of the const ...
The Rat Gene Map
The Rat Gene Map

... map was essential for the characterization of mapping panels of cell hybrids that segregate rat chromosomes, first developed in the mid-1980s (Szpirer and others 1984; Yasue and others 1991). At the time, the rat gene map was quite limited and contained approximately 70 loci that were associated in ...
Nucleotide sequence and taxonomic value of the
Nucleotide sequence and taxonomic value of the

... peptidoglycan (Bavoil et al., 1984). Disulphide interlinking between MOMP polypeptide chains is thought to play an important role in regulating MOMP porin function, nutrient ingress through the EB outer envelope and ultimately the initiation of chlamydial differentiation itself (Bavoil et al., 1984) ...
A strategy for extracting and analyzing large
A strategy for extracting and analyzing large

... mutation must be considered when evaluating the phenotype of the double mutant. Indeed, a double mutant could have a more severe phenotype than either single mutant and still represent a synthetic, neutral, or alleviating interaction. Typically, large-scale studies have scored gene-gene interactions ...
Gene Nomenclature System for Rice
Gene Nomenclature System for Rice

... descriptive of visible phenotypes that provided the earliest evidence for the existence of a gene, and these names and symbols are widely used by the rice research community. With the completion of the rice genome sequence [7] and the emergence of new methods for detecting, characterizing, and descr ...
In Silico method for identification of MHC class I
In Silico method for identification of MHC class I

... (ENSG00000251520). This gene is annotated as a “pseudogene similar to part of major histocompatibility  complex,  class  I‐related  MR1”.  All  these  BLAST  pairs  cluster  with  the  human  MR1  gene,  forming  an  interspecies  groups  distinct  from  CD1  (Figure  4,  Table  S2).  Our  MR1  resu ...
Silent polymorphisms in the RYR1 gene do not modify the
Silent polymorphisms in the RYR1 gene do not modify the

... caused by mutations in the human ryanodine receptor gene (RYR1), which encodes a calcium-release channel. Since the RYR1 gene is huge, containing 106 exons, mutation screening has been limited to three ‘hot spots’, with particular attention to the C-terminal region. Recent next- generation sequencin ...
EMBL-EBI Powerpoint Presentation
EMBL-EBI Powerpoint Presentation

... pathway that supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). ...
ANALYSIS OF MULTIPLE RESTRICTION FRAGMENT LENGTH
ANALYSIS OF MULTIPLE RESTRICTION FRAGMENT LENGTH

... of C3b and C4b (3, 4), a function that might be especially relevant to the finding of a soluble form of CR1 in plasma (5). This regulatory capacity also suggested that CR1 was related to factor H and C4-binding protein (C4BP),' and these three proteins were subsequently shown to share a repeating 60 ...
Exceptionally high levels of recombination
Exceptionally high levels of recombination

... displaying 10 times higher levels of recombination (19 cM/Mb) than previously analyzed genomes of higher eukaryotes. The exceptionally high recombination rate is distributed genome-wide, but varies by two orders of magnitude. Analysis of chromosome, sequence, and gene parameters with respect to reco ...
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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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