Capstone project 2015 in pdf format
... We recently demonstrated resilience of dinoflagellates to DNA damage agents. The present project aims to isolate and characterize dinoflagellate mutants with different genome sizes. The project will involve cell biological and molecular techniques in the generation and monitoring of dinoflagellate l ...
... We recently demonstrated resilience of dinoflagellates to DNA damage agents. The present project aims to isolate and characterize dinoflagellate mutants with different genome sizes. The project will involve cell biological and molecular techniques in the generation and monitoring of dinoflagellate l ...
Determining the cause of patchwork HBA1 and HBA2 genes
... however, remained wildtype HBA1. We therefore conclude that we have identified a variant allele of HBA1 in the heterozygous state in patient AP, whereby a short stretch of IVSII has been substituted with an HBA2-specific IVSII sequence. We refer to this HBA1 variant as a α121 patchwork allele due to ...
... however, remained wildtype HBA1. We therefore conclude that we have identified a variant allele of HBA1 in the heterozygous state in patient AP, whereby a short stretch of IVSII has been substituted with an HBA2-specific IVSII sequence. We refer to this HBA1 variant as a α121 patchwork allele due to ...
Identification of a novel duplication in the APC gene using multiple
... Germline mutations in the adenomatous polyposis coli (APC ) gene cause familial adenomatous polyposis (FAP), an autosomal dominant disease characterized by hundreds to thousands of adenomatous polyps in the colon and rectum, with progression to colorectal cancer. The majority of APC mutations are nu ...
... Germline mutations in the adenomatous polyposis coli (APC ) gene cause familial adenomatous polyposis (FAP), an autosomal dominant disease characterized by hundreds to thousands of adenomatous polyps in the colon and rectum, with progression to colorectal cancer. The majority of APC mutations are nu ...
Garrett-Engele* C. M., Siegal*, M. L., Manoli, D. S., Williams, B. C., Li. H., and Baker, B. S.
... and visualized by staining with ethidium bromide. Hybridization with an antisense ix probe labeled with [α-32P]UTP was carried out overnight in 5×SSPE, 5×Denhardt’s solution, 0.4% SDS, 10 µg/ml salmon sperm DNA, 50% formamide at 60°C. Two washes in 2×SSC/0.1% SDS at room temperature for 15 minutes w ...
... and visualized by staining with ethidium bromide. Hybridization with an antisense ix probe labeled with [α-32P]UTP was carried out overnight in 5×SSPE, 5×Denhardt’s solution, 0.4% SDS, 10 µg/ml salmon sperm DNA, 50% formamide at 60°C. Two washes in 2×SSC/0.1% SDS at room temperature for 15 minutes w ...
About the origins of Molecular Biology and of our Department at UTD
... phage meetings where the new ideas about the nature of the gene were discussed and new experiments conceived. His presence and style attracted many outstanding scientists to the new biology and focused them on fundamental questions. Stent associated the structural and informational school with “thre ...
... phage meetings where the new ideas about the nature of the gene were discussed and new experiments conceived. His presence and style attracted many outstanding scientists to the new biology and focused them on fundamental questions. Stent associated the structural and informational school with “thre ...
For Official Use ENV/JM/BIO(2006)6/REV3 Working
... Natural transformation is generally understood as the uptake of free DNA by competent bacteria (Lorenz and Wackernagel, 1994; Dubnau, 1999, Chen and Dubnau, 2004). Natural competence is a genetically programmed physiological state permitting the efficient uptake of macromolecular DNA. Natural transf ...
... Natural transformation is generally understood as the uptake of free DNA by competent bacteria (Lorenz and Wackernagel, 1994; Dubnau, 1999, Chen and Dubnau, 2004). Natural competence is a genetically programmed physiological state permitting the efficient uptake of macromolecular DNA. Natural transf ...
FLUORESCENT PROTEIN IN Escherichia coli
... AMPs are an essential part of the innate immune system, hence their description as host defence peptides. The innate immune system evolved 2.6 billion years ago, and is present in all living organisms [Gordon et al., 2005]. It is the front-line defense against infections, and it is very rapid compar ...
... AMPs are an essential part of the innate immune system, hence their description as host defence peptides. The innate immune system evolved 2.6 billion years ago, and is present in all living organisms [Gordon et al., 2005]. It is the front-line defense against infections, and it is very rapid compar ...
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... downstream of the BMP-2 gene and spans 18 kb. Previous studies have shown that these three polymorphisms on chromosome 20p12 were associated with the risk of osteoporosis and osteoporotic fracture [19, 20]. The rs996544 (C/T) “TT” genotype and rs965291 (G/A) “AA” genotype were associated with higher ...
... downstream of the BMP-2 gene and spans 18 kb. Previous studies have shown that these three polymorphisms on chromosome 20p12 were associated with the risk of osteoporosis and osteoporotic fracture [19, 20]. The rs996544 (C/T) “TT” genotype and rs965291 (G/A) “AA” genotype were associated with higher ...
A Novel CpG Island Set Identifies Tissue-Specific
... resulting library was sequenced to define a novel human blood CGI set that includes many that are not detected by current algorithms. Approximately half of CGIs were associated with annotated gene transcription start sites, the remainder being intra- or intergenic. Using an array representing over 1 ...
... resulting library was sequenced to define a novel human blood CGI set that includes many that are not detected by current algorithms. Approximately half of CGIs were associated with annotated gene transcription start sites, the remainder being intra- or intergenic. Using an array representing over 1 ...
Epidemiology and control of fungally transmitted cereal viruses
... monoclonal antibodies) cannot be used to distinguish these isolates. A promising new method, single-strand conformation polymorphism analysis of fragments of cDNA amplified by RT-PCR, revealed differences between isolates but none was consistently strain-related. It was therefore decided to proceed ...
... monoclonal antibodies) cannot be used to distinguish these isolates. A promising new method, single-strand conformation polymorphism analysis of fragments of cDNA amplified by RT-PCR, revealed differences between isolates but none was consistently strain-related. It was therefore decided to proceed ...
Cloning of the Papaya Chromoplast-Specific
... Papaya genomic resources have been developed in recent years, including the draft genome sequence and the integrated physical and genetic map (Ming et al., 2008; Yu et al., 2009). Papaya belongs to the order Brassicales and has no whole genome duplication (WGD) event since the paleohexaploidy event ...
... Papaya genomic resources have been developed in recent years, including the draft genome sequence and the integrated physical and genetic map (Ming et al., 2008; Yu et al., 2009). Papaya belongs to the order Brassicales and has no whole genome duplication (WGD) event since the paleohexaploidy event ...
Cloning and expression of the phosphotriesterase
... Increased expression of hocA was observed from an integrative hocA–lacZ fusion when cultures were grown in the absence of phosphate, suggesting that it might be part of the Pho regulon, but the phosphate-regulated promoter was not cloned in this study. This is believed to be the first study in which ...
... Increased expression of hocA was observed from an integrative hocA–lacZ fusion when cultures were grown in the absence of phosphate, suggesting that it might be part of the Pho regulon, but the phosphate-regulated promoter was not cloned in this study. This is believed to be the first study in which ...
Towards an accurate identification of mosaic genes and
... imposed by the necessity for taxa involved in HGT to be contemporaneous (6,18,20). Fix the sliding window size w and the step size s. In our experiments, the window sizes of l/5, l/4, l/3 and l/2 sites and the sliding window progress step of 10 sites were used. Step k. Fix the position of the slidin ...
... imposed by the necessity for taxa involved in HGT to be contemporaneous (6,18,20). Fix the sliding window size w and the step size s. In our experiments, the window sizes of l/5, l/4, l/3 and l/2 sites and the sliding window progress step of 10 sites were used. Step k. Fix the position of the slidin ...
genes is conserved among species related to
... possesses two S-protein-encoding genes, one of which is silent, on a chromosomal segment of 6 kb. The 5-protein-encoding gene in the expression site can be exchanged for the silent S-protein-encoding gene by inversion of t h i s slp segment. In this study the presence of S-protein and corresponding ...
... possesses two S-protein-encoding genes, one of which is silent, on a chromosomal segment of 6 kb. The 5-protein-encoding gene in the expression site can be exchanged for the silent S-protein-encoding gene by inversion of t h i s slp segment. In this study the presence of S-protein and corresponding ...
Geneious Sequence Classifier User Manual
... sequence will be classified to the taxonomic level that these database sequences have in common. E.g. for the example dataset given above with ”classify using taxonomy” checked, if this value is set on 0.2%, and the query sequence has 99.3% identity with Acmopyle pancheri and 99.2% identity with Acm ...
... sequence will be classified to the taxonomic level that these database sequences have in common. E.g. for the example dataset given above with ”classify using taxonomy” checked, if this value is set on 0.2%, and the query sequence has 99.3% identity with Acmopyle pancheri and 99.2% identity with Acm ...
Lesson Overview - mr. welling` s school page
... With cell membranes unable to transport chloride ions, tissues throughout the body malfunction. Children with CF have serious digestive problems and produce thick, heavy mucus that clogs their lungs and breathing passageways. ...
... With cell membranes unable to transport chloride ions, tissues throughout the body malfunction. Children with CF have serious digestive problems and produce thick, heavy mucus that clogs their lungs and breathing passageways. ...
Engineered bacteriophage-defence systems in bioprocessing
... structural proteins. These polymers are then assembled Induction into mature phage particles and released by a cell-lysis event that is coordinated by the action of two phageencoded proteins, holin and endolysin30,31. Some of these reactions rely heavily on the host’s replication and Assembly Subver ...
... structural proteins. These polymers are then assembled Induction into mature phage particles and released by a cell-lysis event that is coordinated by the action of two phageencoded proteins, holin and endolysin30,31. Some of these reactions rely heavily on the host’s replication and Assembly Subver ...
The Large Loop Repair and Mismatch Repair Pathways
... surrounding the lesion is unwound. The single-stranded DNA containing the lesion is removed by two endonucleases. A heterodimeric complex, consisting of Rad1p and Rad10p, cuts the damaged DNA strand on the 5⬘side of the lesion, while Rad2p cuts on the 3⬘-side of the lesion. These cuts result in the ...
... surrounding the lesion is unwound. The single-stranded DNA containing the lesion is removed by two endonucleases. A heterodimeric complex, consisting of Rad1p and Rad10p, cuts the damaged DNA strand on the 5⬘side of the lesion, while Rad2p cuts on the 3⬘-side of the lesion. These cuts result in the ...
i. Genetics
... The kind of cell which is formed, (muscle, blood, nerve etc.) is controlled by DNA The kind of organism which is produced (giraffe, herring, human, etc.) is controlled by DNA ...
... The kind of cell which is formed, (muscle, blood, nerve etc.) is controlled by DNA The kind of organism which is produced (giraffe, herring, human, etc.) is controlled by DNA ...
Alternative Splicing
... Humans have only twice as many genes as the simple fruit fly. How can this be? After all, we have brains that allow us to count genes while their main occupation is seeking out a nice banana. Alternative splicing explains the compact living of our genetic information as well as the mechanisms behind ...
... Humans have only twice as many genes as the simple fruit fly. How can this be? After all, we have brains that allow us to count genes while their main occupation is seeking out a nice banana. Alternative splicing explains the compact living of our genetic information as well as the mechanisms behind ...
Genetics of Down Syndrome
... small structural aberrations with the results of different gene product analyses (dosage effect). Chromosome 21 was sequenced in 2000 (Hattori et al., 2000), and 225 loci (genes) were identified, which was less than expected. This might explain the relatively mild phenotype of the carriers. In the f ...
... small structural aberrations with the results of different gene product analyses (dosage effect). Chromosome 21 was sequenced in 2000 (Hattori et al., 2000), and 225 loci (genes) were identified, which was less than expected. This might explain the relatively mild phenotype of the carriers. In the f ...
Rich Probabilistic Models for Genomic Data
... To account for the genome-wide search, compare the observed LOD scores to the null distribution of the maximum LOD score, genome-wide, that would be obtained if there were no QTL anywhere. LOD threshold = 95th percentile of the distribution of genome-wide max LOD, when there are no QTL ...
... To account for the genome-wide search, compare the observed LOD scores to the null distribution of the maximum LOD score, genome-wide, that would be obtained if there were no QTL anywhere. LOD threshold = 95th percentile of the distribution of genome-wide max LOD, when there are no QTL ...
Simplified Insertion of Transgenes Onto Balancer Chromosomes via
... Figure 2 Insertion of donor cassettes onto balancers supports expression of transgenes. (A) Schematic of the exchange reaction. Integrase-mediated crossovers at both ends of the aligned target and donor cassettes result in removal of mini-white and integration of the gene of interest (GOI) into the ...
... Figure 2 Insertion of donor cassettes onto balancers supports expression of transgenes. (A) Schematic of the exchange reaction. Integrase-mediated crossovers at both ends of the aligned target and donor cassettes result in removal of mini-white and integration of the gene of interest (GOI) into the ...
Microarrays - Consortium for Mathematics and its Applications
... four feet tall. A mutation is a permanent change in the genetic material, usually in a single gene that may be transmitted from one generation to the next. Learning which genes may be involved in genetic diseases like dwarfism has many possible benefits for people. Understanding how DNA functions is ...
... four feet tall. A mutation is a permanent change in the genetic material, usually in a single gene that may be transmitted from one generation to the next. Learning which genes may be involved in genetic diseases like dwarfism has many possible benefits for people. Understanding how DNA functions is ...